We narrowed to 63,890 results for: cell

1. pcDNA3-HA-MKRN1 H307E

   Plasmid

   #78749

   Purpose

   To overexpress MKRN1 H307E in Mammalian Cell

   Depositor

   Jaewhan Song

   Article

   Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

   Insert

   MKRN1 (MKRN1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   H307E

   Promoter

   CMV

   Available Since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. Nano-lantern-Actin/pEGFP-C1

   Plasmid

   #51972

   Purpose

   mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, fused to actin

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern
   

   Tags

   Actin

   Expression

   Mammalian

   Mutation

   S257G in Rluc8

   Available Since

   March 24, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pLJC2-UMPS (Y37A, R155A)-3xFLAG

   Plasmid

   #87976

   Purpose

   Expression of UMPS (Y37A, R155A) in mammalian cells

   Depositor

   David Sabatini

   Article

   Cantor et al Cell. 2017 Apr 6;169(2):258-272.e17. doi

   Insert

   UMPS (Y37A, R155A) (UMPS Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Y37A, R155A

   Promoter

   CMV

   Available Since

   April 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLJC2-Rap2A-3xFLAG

   Plasmid

   #87974

   Purpose

   3rd generation lentiviral vector for Expression of Rap2A in mammalian cells

   Depositor

   David Sabatini

   Article

   Cantor et al Cell. 2017 Apr 6;169(2):258-272.e17. doi

   Insert

   RAP2A (RAP2A Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS EFF-1::V5

   Plasmid

   #133584

   Purpose

   Expresses EFF-1 A full length protein in mammalian cells

   Depositor

   Benjamin Podbilewicz

   Article

   Avinoam et al Science. 2011 Apr 29;332(6029):589-92. d

   Insert

   EFF-1::V5
   

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. Nano-lantern-H2B/pcDNA3

   Plasmid

   #51971

   Purpose

   mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, fused to Histone H2B

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern
   

   Tags

   human H2B

   Expression

   Mammalian

   Mutation

   S257G in Rluc8

   Available Since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pLEX-FHH-MAPKAP1RBD/Triple Mutant

   Plasmid

   #120711

   Purpose

   Expresses Flag-HA-6xHIS-MAPKAP1 RBD Triple Mutant fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   MAPKAP1 RBD
   

   Use

   Lentiviral

   Tags

   Flag-HA-6xHIS

   Expression

   Mammalian

   Mutation

   Amino acids 279-353, K307A, K310A, R312L

   Promoter

   CMV

   Available Since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLEX-MAPKAP1Del-FHH-IRES-Puro

   Plasmid

   #120706

   Purpose

   Expresses MAPKAP1 Truncation-Flag-HA-6xHIS fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   MAPKAP1 Isoform 1
   

   Use

   Lentiviral

   Tags

   Flag-HA-6xHIS

   Expression

   Mammalian

   Mutation

   Deleted amino acids 193-522

   Promoter

   CMV

   Available Since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. EK0387 SFFV-mTagBFP2-SGc(Bdnf.s1)cSG-mCherry (FLP-IN)

   Plasmid

   #191157

   Purpose

   Expression of a sensor for murine Bdnf 3' UTR in mammalian cells; mTagBFP2 marker, mCherry output; 90 bp long

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   mTagBFP2:Bdnf.s1:mCherry
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. PM18033-PLS-KP6_rpoA-C

    Plasmid

    #190986

    Purpose

    Test the C-to-U editing activity of KP6 on AtrpoA editing site, in human cells

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP6
    

    Tags

    His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PM18033-PLS-KP2_rpoA-U

    Plasmid

    #190976

    Purpose

    Test the U-to-C editing activity of KP2 on AtrpoA editing site, in human cells

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP2
    

    Tags

    His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PM18033-PLS-KP3_rpoA-U

    Plasmid

    #190980

    Purpose

    Test the U-to-C editing activity of KP3 on AtrpoA editing site, in human cells

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP3
    

    Tags

    His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLib6.4B-Aaeg_774

    Plasmid

    #176673

    Purpose

    Expression of sgRNA under Ae. Aegypti U6 (AAEL017774) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Expression

    Insect

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLib6.4B-Cquin_596

    Plasmid

    #176669

    Purpose

    Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039596) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Expression

    Insect

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. NK-1 GFP

    Plasmid

    #24508

    Depositor

    Ron Vale

    Article

    Case et al Cell. 1997 Sep 5. 90(5):959-66.

    Insert

    Drosophila NCD, human kinesin
    

    Tags

    GFP and His

    Expression

    Bacterial

    Mutation

    NK-1 is a chimera where the entire superfamily-co…

    Available Since

    June 30, 2010

    Availability

    Academic Institutions and Nonprofits only

16. WNT11-bio-His

    Plasmid

    #53430

    Purpose

    Expresses full-length Protein Wnt-11 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    WNT11 (WNT11 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCK-D390TN(UniProt)-FLAG

    Plasmid

    #164693

    Purpose

    Express N-terminally truncated DroshaTN-FLAG in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Insert

    N-terminally truncated transdominant negative Drosha (DROSHA Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5-MIS12-LAP-BUB1

    Plasmid

    #115904

    Purpose

    Used for expression of siRNA-resistant MIS12-BUB1 from the FRT site of FlpIn cells upon addition of doxycyclin.

    Depositor

    Geert Kops

    Article

    Vleugel et al J Cell Sci. 2015 Aug 15;128(16):2975-82.

    Insert

    MIS12-BUB1 (MIS12 Human)
    

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    BUB1 fused to MIS12

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOPTXcGMPRELUC

    Plasmid

    #68503

    Purpose

    cyclic nucleotide reporter for bacteria or plant cells

    Depositor

    Helen Irving

    Article

    Wheeler et al BMC Biotechnol. 2013 Nov 9;13:97. doi: 1

    Insert

    OPTX promoter with cGMPRE (AT1G33440 Mustard Weed)
    

    Tags

    luciferase

    Expression

    Bacterial and Plant

    Mutation

    3x cGMPRE inserted 190bp 5' of ATG (cGMPRE =…

    Promoter

    OPTX promoter with cGMPRE

    Available Since

    Sept. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pME-hygro-hPGAP3-3HA

    Plasmid

    #50373

    Purpose

    Express C-terminally triple HA tagged human PGAP3 in mammmalian cells

    Depositor

    Reika Watanabe

    Article

    Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

    Insert

    PGAP3 post-GPI attachment to proteins 3 (PGAP3 Human)
    

    Tags

    3x HA

    Expression

    Mammalian

    Promoter

    SR alpha

    Available Since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only