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GFP-RIAM(1-666)

Plasmid

#80028

Purpose

Mammalian expression of GFP-RIAM(1-666)

Depositor

Chinten James Lim

Article

Lee et al Mol Biol Cell. 2013 May;24(9):1354-62. d

Insert

RIAM (APBB1IP Human)

Tags

EGFP

Expression

Mammalian

Mutation

aa 1-666

Promoter

CMV

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only
pSMP-G9A_4

Plasmid

#36334

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

G9A (EHMT2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pCDNAIIIB cdc42 WT

Plasmid

#61635

Purpose

mammalian expression of cdc42 wt

Depositor

JS Gutkind

Article

Coso et al Cell. 1995 Jun 30;81(7):1137-46.

Insert

cdc42 (CDC42 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pDGE474

Plasmid

#153265

Purpose

M1 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE475

Plasmid

#153266

Purpose

M2 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE479

Plasmid

#153270

Purpose

M4E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pJK_ToeRepG2_N02_switch

Plasmid

#132742

Purpose

T7 RNAP-driven expression of second-generation toehold repressor index 2 switch RNA with a GFP-ASV reporter

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

ToeRepG2_N02_switch

Expression

Bacterial

Available Since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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DT049A

Plasmid

#159410

Purpose

Constitutive murine mature BDNF expression, controlled under E.coli tet promoter

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.tet-BDNF (Bdnf, Mouse) (Bdnf Mouse, Synthetic)

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Promoter

tet promoter

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-Miro1 S432N

Plasmid

#47893

Purpose

Expresses myc tagged Miro1 S432N dominant negative mutant

Depositor

Pontus Aspenström

Article

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Insert

Miro1 S432N (RHOT1 Human)

Tags

myc

Expression

Mammalian

Mutation

S432N, dominant negative mutant

Promoter

CMV

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only
mTFP1-Clathrin-15

Plasmid

#55471

Purpose

Localization: Clathrin Vesicles, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Clathrin (CLTB Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
pBacT7-S1T1L

Plasmid

#33292

Depositor

Terence Dermody

Article

Kobayashi et al Cell Host Microbe. 2007 Apr 19;1(2):147-

Insert

S1 (MoV1sS1gp2, MoV1sS1gp1 Orthoreovirus T1L)

Expression

Mammalian

Promoter

T7

Available Since

Dec. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pT7-L1T1L

Plasmid

#33286

Depositor

Terence Dermody

Article

Kobayashi et al Cell Host Microbe. 2007 Apr 19;1(2):147-

Insert

L1 (L1 Orthoreovirus T1L)

Expression

Mammalian

Promoter

T7

Available Since

Dec. 5, 2011

Availability

Academic Institutions and Nonprofits only

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plenti-arRNA-BFP-backbone

Plasmid

#180221

Purpose

For the cloning of arRNA-expressing vectors containing BFP fluorescence marker in mammalian cells

Depositor

Wensheng Wei

Article

Qu et al Nat Biotechnol. 2019 Sep;37(9):1059-1069

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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sABE v3.22 Lenti_1

Plasmid

#209555

Purpose

pCMV-SV40NLS-2xFKBP3-TadA-8e(77-167)-SpCas9(D10A, 2-468)-gp41-1_IntN

Depositor

Xue Gao

Article

Zeng et al Nat Commun. 2023 Sep 11;14(1):5573. doi:

Insert

2xFKBP3-TadA-8e(77-167)-SpCas9(D10A, 2-468)-gp41-1_IntN

Expression

Mammalian

Promoter

pCMV

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pRSFDuet1-His-TEV-OTC_Ub

Plasmid

#172134

Purpose

Scaﬀold design for studying ubiquitin interacting proteins by cryo-EM.

Depositor

Shang-Te Danny Hsu

Article

Chiu et al Biochemistry. 2021 Apr 13;60(14):1075-10

Insert

E. coli ornithine transcarbamylase (OTC), fused to human ubiquitin

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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Talin1 head-mCherry

Plasmid

#212005

Purpose

Expression of mouse talin1 head (1-490)-mCherry in mammalian cells

Depositor

Vesa Hytönen

Article

Rahikainen et al J Cell Sci. 2019 Apr 3;132(7):jcs226514.

Insert

Mouse talin1 head domain (1-490) (Tln1 Mouse)

Tags

mCherry fluorescent protein

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV5 MEKK4alpha KD

Plasmid

#12188

Depositor

Gary Johnson

Article

Gerwins et al J Biol Chem. 1997 Mar 28. 272(13):8288-9

Insert

MEKK4 (Map3k4 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Kinase dead: K1361M

Available Since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only
pENeGFP PCNAL2 pc653

Plasmid

#167564

Purpose

The GFP-PCNAL2 fusion protein contains a SV40 nuclear localization signal at the NH2 terminus followed by an enhanced mutant GFP gene that is fused to the human PCNA by a linker.

Depositor

Cristina Cardoso

Article

Leonhardt et al J Cell Biol. 2000 Apr 17;149(2):271-80.

Insert

Proliferating Cell Nuclear Antigen (PCNA Human)

Tags

GFP

Expression

Mammalian

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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mADAM17deltaMP in pQCXIP cFLAG

Plasmid

#19144

Depositor

Zena Werb

Article

Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

Insert

ADAM17 (Adam17 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

delta 189-378

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pY012 (pcDNA3.1-hCMtCpf1)

Plasmid

#69984

Purpose

Expresses humanized CMtCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hCMtCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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