19,613 results

1. pKP332 (Lenti-OSK1)

   Plasmid

   #21627

   Depositor

   Tim Townes

   Article

   Chang et al Stem Cells. 2009 May . 27(5):1042-9.

   Insert

   hOCT4 - 2A - hSOX2 - 2A - hKLF4 (POU5F1, SOX2, KLF4 Human, Porcine teschovirus-1)
   

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. SPLICS LY-MT Long P2A

   Plasmid

   #213613

   Purpose

   Detect the long-range Lysosome-Mitochondria contact

   Depositor

   Tito Calì

   Article

   Giamogante et al Nat Commun. 2024 Feb 19;15(1):1516. doi:

   Insert

   SPLICS LY-MT Long P2A
   

   Expression

   Mammalian

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. eGFP-ova-C1

   Plasmid

   #163524

   Purpose

   Expresses both eGFP and chicken ovalbumin in mammalian cells

   Depositor

   Thomas Weber

   Insert

   enhanced-GFP-chicken ovalbumin tandem
   

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGWB501

   Plasmid

   #74843

   Purpose

   Gateway cloning compatible binary vector for simple cloning of gene.

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   No Promoter

   Available Since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRCT

   Plasmid

   #60621

   Purpose

   Plasmid encoding iCas9, tracrRNA and crRNAs

   Depositor

   Huimin Zhao

   Article

   Bao et al ACS Synth Biol. 2014 Sep 19.

   Inserts

   iCas9
   tracrRNA
   

   Use

   CRISPR

   Tags

   FLAG and SV40 NLS

   Expression

   Yeast

   Mutation

   changed Aspartate 147 to Tyrosine, Proline 411 to…

   Promoter

   RPR1p and TEF1p

   Available Since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti6-DEST PINK1-V5 WT

   Plasmid

   #13320

   Depositor

   Mark Cookson

   Article

   Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

   Insert

   PINK1 (PINK1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pG1K

   Plasmid

   #71741

   Purpose

   Modular shuttle vector for Geobacillus and E. coli

   Depositor

   Tom Ellis

   Article

   Reeve et al ACS Synth Biol. 2016 Jul 1.

   Type

   Empty backbone

   Use

   Synthetic Biology; Shuttle vector

   Expression

   Bacterial

   Available Since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCW57.1-4EBP1_4xAla

   Plasmid

   #38240

   Depositor

   David Sabatini

   Article

   Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

   Insert

   4EBP1 (Eif4ebp1 Rat, hybrid gene of rat and human)
   

   Use

   Lentiviral; Doxycycline inducible

   Expression

   Mammalian

   Mutation

   T37A, T46A, S65A, T70A (dominant negative)

   Promoter

   Tet ON

   Available Since

   Nov. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. plRL117

   Plasmid

   #163635

   Purpose

   Optimized M. smegmatis CRISPRi plasmid; L5 integrating.

   Depositor

   Jeremy Rock

   Article

   Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   Tet-On

   Available Since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. SPLICS LY-MT Short P2A

    Plasmid

    #213614

    Purpose

    Detect the short-range Lysosome-Mitochondria contact

    Depositor

    Tito Calì

    Article

    Giamogante et al Nat Commun. 2024 Feb 19;15(1):1516. doi:

    Insert

    SPLICS LY-MT Short P2A
    

    Expression

    Mammalian

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. hAAVS1 1R TALEN

    Plasmid

    #35432

    Purpose

    TALEN designed to target the human AAVS1 locus

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

    Insert

    hAAVS1 1R TALEN
    

    Expression

    Mammalian

    Available Since

    April 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. hAAVS1 1L TALEN

    Plasmid

    #35431

    Purpose

    TALEN designed to target the human AAVS1 locus

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

    Insert

    hAAVS1 1L TALEN
    

    Expression

    Mammalian

    Available Since

    May 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. NA20 LN16

    Plasmid

    #101870

    Purpose

    Expresses HIV Env gene when transfected into 293-T cells for production of pseudovirus

    Depositor

    Paul Clapham

    Article

    Peters et al J Virol. 2004 Jul;78(13):6915-26.

    Insert

    Env (env )
    

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

14. pRK5-myc-Miro1

    Plasmid

    #47888

    Purpose

    Expresses myc tagged Miro1

    Depositor

    Pontus Aspenström

    Article

    Fransson et al J Biol Chem. 2003 Feb 21;278(8):6495-502

    Insert

    Miro1 (RHOT1 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMK345 (Hygro-P2A-mAID-mClover)

    Plasmid

    #121179

    Purpose

    N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    Hygro-P2A-mAID-mClover
    

    Use

    CRISPR

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCI-neo-sOVA

    Plasmid

    #25098

    Depositor

    Maria Castro

    Article

    Yang et al Proc Natl Acad Sci U S A. 2010 Mar 9. 10

    Insert

    soluble ovalbumin
    

    Expression

    Bacterial and Mammalian

    Available Since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYFP-RPB1aAmr

    Plasmid

    #75284

    Purpose

    Expresses alpha-amanitin resistant EYFP tagged POLR2A in mammalian cells

    Depositor

    Xavier Darzacq, Robert Singer

    Article

    Darzacq et al Nat Struct Mol Biol. 2007 Sep;14(9):796-

    Insert

    POLR2A (POLR2A Human)
    

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    N792D alpha-amanitin–resistant

    Promoter

    CMV

    Available Since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDH-EF1-FHC

    Plasmid

    #64874

    Purpose

    lentiviral vector with Flag, HA tags

    Depositor

    Richard Wood

    Article

    Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. 5'UTR CGG 99x FMR1-EGFP

    Plasmid

    #63091

    Purpose

    Contains expanded CGG repeats (99 repeats) within the 5'UTR of FMR1 as found in the Fragile X Tremor Ataxia Syndrome (FXTAS). These repeats are in frame with EGFP.

    Depositor

    Nicolas Charlet-Berguerand

    Insert

    expanded CGG repeats (99 repeats) within the 5'UTR of FMR1 (FMR1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-MCS2

    Plasmid

    #46954

    Purpose

    Cloning plasmid for the generation of Knock-In in human cells using rAAV.

    Depositor

    Steve Jackson

    Article

    Britton et al J Cell Biol. 2013 Jul 29.

    Type

    Empty backbone

    Use

    AAV

    Available Since

    Sept. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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