We narrowed to 15,742 results for: grna

1. FGFR3 gRNA (BRDN0001147930)

   Plasmid

   #75730

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human FGFR3

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   FGFR3 (FGFR3 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330_ACTB-i1 sgRNA / hSpCas9

   Plasmid

   #172825

   Purpose

   Mammalian expression of a sgRNA targeting the intron 1 position 1 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. px459-RhebL1 sgRNA

   Plasmid

   #133769

   Purpose

   Expresses Cas9 and human RhebL1 sgRNA

   Depositor

   Shawn Ferguson

   Article

   Angarola et al Mol Biol Cell. 2019 Oct 15;30(22):2750-2

   Insert

   RhebL1 sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Nov. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti-SLC35B2-sgRNA

   Plasmid

   #154860

   Purpose

   Lentiviral expression of Cas9 and sgRNA targeting SLC35B2

   Depositor

   Yihong Ye

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

   Insert

   SLC35B2 sgRNA (SLC35B2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   July 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. CDK7 gRNA (BRDN0001146106)

   Plasmid

   #76078

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CDK7

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   CDK7 (CDK7 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pX459-HypaCas9-TUBA1B_sgRNA

   Plasmid

   #183889

   Purpose

   pX459V2.0-HypaCas9 plasmid with TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   TUBA1B sgRNA spacer (TUBA1B Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Plas-gRNA-LEU2

   Plasmid

   #157656

   Purpose

   GFP and gRNA tag for protein and mRNA quantification, to attache to the 3' end of the gene coding sequence

   Depositor

   Frank Albert

   Article

   Brion et al Elife. 2020 Nov 16;9. pii: 60645. doi: 1

   Insert

   GFP-gRNA
   

   Use

   Insert storage (replicative in e. coli)

   Tags

   GFP-gRNA

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. CAD gRNA (BRDN0001146638)

   Plasmid

   #76962

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CAD

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   CAD (CAD Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. lentiCRISPR - DDX3Y sgRNA 2

   Plasmid

   #70657

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a DDX3Y targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   sgRNA against DDX3Y
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

10. sgRNA BRCA1 C64Y

    Plasmid

    #139326

    Purpose

    Plasmid expressing a sgRNA to introduce BRCA1 C64Y using base editing

    Depositor

    Alberto Ciccia

    Article

    Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

    Insert

    sgRNA to insert BRCA1 C64Y using base editing
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. sgRNA BRCA1 E638K

    Plasmid

    #139327

    Purpose

    Plasmid expressing a sgRNA to introduce BRCA1 E638K using base editing

    Depositor

    Alberto Ciccia

    Article

    Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

    Insert

    sgRNA to insert BRCA1 E638K using base editing
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. BRD4 gRNA (BRDN0001146786)

    Plasmid

    #77346

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human BRD4

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    BRD4 (BRD4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ATAD2 D7.3 gRNA

    Plasmid

    #90538

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ATAD2

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    ATAD2 (Guide Designation D7.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. hgRNA-F21_pLKO-Hyg

    Plasmid

    #100564

    Purpose

    hgRNA-F21 in a lentiviral backbone

    Depositor

    George Church

    Article

    Kalhor et al Nat Methods. 2017 Feb;14(2):195-200. doi

    Insert

    hgRNA-F21
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJL1-mRFP1 gRNA

    Plasmid

    #117052

    Purpose

    In vitro expression of anti-mRFP1 Cas9 sgRNA from the T7 promoter

    Depositor

    Michael Jewett

    Article

    Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

    Insert

    anti-mRFP1 SpCas9 gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ERN2 gRNA (BRDN0001145316)

    Plasmid

    #75845

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ERN2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ERN2 (ERN2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDONR-U6gRNA-PGKpuro2ABFP

    Plasmid

    #136405

    Purpose

    Donor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses Puromycin linked to TagBFP.

    Depositor

    Massimo Squatrito

    Article

    Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    BbsI restriction site at position 437 was removed…

    Promoter

    U6, PDK

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. PI4K2A gRNA (BRDN0001147595)

    Plasmid

    #76822

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PI4K2A

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PI4K2A (PI4K2A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRPR1(1xTetO)_gRNA_handle_RPR1t

    Plasmid

    #62966

    Purpose

    aTc inducible gRNA expression vector

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    RPR1

    Available Since

    March 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EIF2AK3 gRNA (BRDN0001146966)

    Plasmid

    #77168

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human EIF2AK3

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EIF2AK3 (EIF2AK3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. RIPK1 gRNA (BRDN0001146967)

    Plasmid

    #76534

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RIPK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RIPK1 (RIPK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. RIPK1 gRNA (BRDN0001149147)

    Plasmid

    #76535

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RIPK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RIPK1 (RIPK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. LRRK2 gRNA (BRDN0001146754)

    Plasmid

    #77330

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human LRRK2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCfB9337 (pgRNA_IV-1_NatMX)

    Plasmid

    #161590

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site IV-1

    Depositor

    Irina Borodina

    Article

    Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

    Insert

    guiding RNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. PDPK1 gRNA (BRDN0001146405)

    Plasmid

    #77453

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PDPK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PDPK1 (PDPK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. hgRNA-A51_pLKO-Hyg

    Plasmid

    #100567

    Purpose

    hgRNA-A51 in a lentiviral backbone

    Depositor

    George Church

    Article

    Kalhor et al Nat Methods. 2017 Feb;14(2):195-200. doi

    Insert

    hgRNA-A51
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PLK4 gRNA (BRDN0001146388)

    Plasmid

    #76234

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PLK4

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PLK4 (PLK4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CDK1 gRNA (BRDN0001147731)

    Plasmid

    #77388

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CDK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CDK1 (CDK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. CCL2 gRNA (BRDN0001148290)

    Plasmid

    #77867

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CCL2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CCL2 gRNA (CCL2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSpCas9_BB_2A-GFP_MAPRE1-gRNA#2

    Plasmid

    #107727

    Purpose

    Knock out of EB1 in human cells by CRISPR/Cas9

    Depositor

    Torsten Wittmann

    Article

    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

    Insert

    MAPRE1 gRNA #1 (targets Exon 1) (MAPRE1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge