We narrowed to 4,062 results for: p2a

1. AAVS1-TRE3G-SOX2-3xHA-P2A-tagBFP

   Plasmid

   #163701

   Purpose

   Dox-inducible SOX2-3xHA HDR knock-in cassette into the AAVS1 locus with a tagBFP fluorescent marker linked by a self-cleaving P2A peptide.

   Depositor

   Jennifer Mitchell

   Article

   Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

   Inserts

   SOX2-3xHA-P2A-tagBFP (SOX2 Human)
   rtTA
   T2-sgRNA
   

   Use

   AAV and CRISPR

   Tags

   3x-HA and P2A-tagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and TRE3G

   Available since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMX-Sox17-p2A-Erg-t2A-GFP

   Plasmid

   #222496

   Purpose

   mouse fibroblast-to-endothelial cell direct reprogramming

   Depositor

   Li Qian

   Article

   Farber et al Nat Commun. 2024 May 16;15(1):4170. doi:

   Inserts

   Sox17 (Sox17 Mouse)
   Erg (Erg )
   EGFP
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV:: PDGFR-Cry2olig-FRB-P2A-GFP(1-10)

   Plasmid

   #221776

   Purpose

   Expresses PDGFR-Cry2olig-FRB-P2A-GFP(1-10) component in mammalian cells

   Depositor

   Dongmin Lee

   Article

   Lee et al iScience. 2023 Feb 19;26(3):106233. doi:

   Insert

   pCMV:: PDGFR-Cry2olig-FRB-P2A-GFP(1-10)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5-FRT-TO-NUbo-E3(48)-P2A-3xFLAG-Ubc7

   Plasmid

   #212804

   Purpose

   Constitutive or doxycycline-inducible expression of NUbo-E3(48)-P2A-3xFLAG-Ubc7 in mammalian cells

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   NUbo-E3(48)-P2A-3xFLAG-Ubc7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. SOX2-P2A-NLS-mClover3-IRES-Puro

   Plasmid

   #215544

   Purpose

   Donor plasmid for knock-in P2A-NLS-mClover3 into the human SOX2 locus

   Depositor

   Kai Wang

   Insert

   SOX2 homologous recombination arms with IRES-Puro selection cassette (SOX2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-cFOS-FRB-VP16-P2A-EGFP

   Plasmid

   #210510

   Purpose

   expresses cFOS-FRB-VP16 and EGFP component in rat hippocampal neuron cells

   Depositor

   Dongmin Lee

   Article

   Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

   Insert

   FRB-VP16-P2A-EGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   cFos

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-h.Rhno1 Cdk1mut7A-C-Myc-P2A-Puro

   Plasmid

   #211617

   Purpose

   Rhno1 Cdk1mut7A

   Depositor

   Agnel Sfeir

   Article

   Brambati et al Science. 2023 Aug 11;381(6658):653-660.

   Insert

   hRHNO1 (RHNO1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S58A, S98A, S133A, T159A, T198A, S191A, and T202A

   Promoter

   CMV

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS-iRFP713(C15S/V256C)-P2A-mCherry

   Plasmid

   #198069

   Purpose

   Mammalian expression of iRFP713(C15S/V256C) and EGFP

   Depositor

   Michiyuki Matsuda

   Insert

   iRFP713(C15S/V256C)
   

   Use

   Tags

   P2A-mCherry

   Expression

   Mammalian

   Mutation

   C15S/V256C mutation

   Promoter

   CAG

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCSIIbleo-miRFP670nano(R57C/C86S)-P2A-EGFP

   Plasmid

   #197370

   Purpose

   The lentiviral vector for miRFP670nano(R57C/C86S) and EGFP

   Depositor

   Michiyuki Matsuda

   Insert

   miRFP670nano(R57C/C86S)
   

   Use

   Lentiviral

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   R57C/C86S mutation

   Promoter

   CAG

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCSIIbleo-iRFP713(C15S/V256C)-P2A-EGFP

    Plasmid

    #198062

    Purpose

    The lentiviral vector for iRFP713(C15S/V256C) and EGFP

    Depositor

    Michiyuki Matsuda

    Insert

    iRFP713(C15S/V256C)
    

    Use

    Lentiviral

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    C15S/V256C mutation

    Promoter

    CAG

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. LV-PGK-PVR-P2A-Cre-EFS-mScarletSIIN

    Plasmid

    #172435

    Purpose

    Lentivirus, expresses Cre recombinase, mouse PVR (CD155), and mScarlet-SIINFEKL

    Depositor

    Tyler Jacks

    Article

    Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

    Inserts

    Cre
    PVR
    mScarlet-SIINFEKL(OVA257-264)+Ova 323-339
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAc5.1B-lambdaN-HA-DmPop2a-4xmut-dsRNAres-V5His6_R

    Plasmid

    #147435

    Purpose

    Insect Expression of DmPop2a-4xmut-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

    Insert

    DmPop2a-4xmut-dsRNAres (Pop2 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAc5.1B-lambdaN-HA-DmPop2a-5xmut-dsRNAres-V5His6_R

    Plasmid

    #147436

    Purpose

    Insect Expression of DmPop2a-5xmut-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

    Insert

    DmPop2a-5xmut-dsRNAres (Pop2 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAc5.1B-lambdaN-HA-DmPop2a-C80EL84E-dsRNAres-V5His6_R

    Plasmid

    #147437

    Purpose

    Insect Expression of DmPop2a-C80EL84E-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

    Insert

    DmPop2a-C80EL84E-dsRNAres (Pop2 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAc5.1B-lambdaN-HA-DmPop2a-C80EL84EE151A-dsRNAres-V5His6_R

    Plasmid

    #147438

    Purpose

    Insect Expression of DmPop2a-C80EL84EE151A-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

    Insert

    DmPop2a-C80EL84EE151A-dsRNAres (Pop2 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAc5.1B-lambdaN-HA-DmPop2a-E151A-dsRNAres-V5His6_R

    Plasmid

    #147363

    Purpose

    Insect Expression of DmPop2a-E151A-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

    Insert

    DmPop2a-E151A-dsRNAres (Pop2 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAc5.1B-lambdaN-HA-DmPop2a-E162A-dsRNAres-V5His6_R

    Plasmid

    #147364

    Purpose

    Insect Expression of DmPop2a-E162A-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

    Insert

    DmPop2a-E162A-dsRNAres (Pop2 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAc5.1B-lambdaN-HA-DmPop2a-S155A-dsRNAres-V5His6_R

    Plasmid

    #147365

    Purpose

    Insect Expression of DmPop2a-S155A-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

    Insert

    DmPop2a-S155A-dsRNAres (Pop2 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAc5.1B-lambdaN-HA-DmPop2a-D53AE55AE151A-dsRNAres-V5His6_R

    Plasmid

    #147362

    Purpose

    Insect Expression of DmPop2a-D53AE55AE151A-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

    Insert

    DmPop2a-D53AE55AE151A-dsRNAres (Pop2 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-CMV-Hsp1-Hsp2Cas9-P2A-puro

    Plasmid

    #192131

    Purpose

    Expresses Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

    Depositor

    Yongming Wang

    Article

    Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

    Insert

    Hsp1-Hsp2Cas9
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-CMV-Hsp1-Hsp2Cas9-KY-P2A-puro

    Plasmid

    #192133

    Purpose

    Expresses highly specific Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

    Depositor

    Yongming Wang

    Article

    Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

    Insert

    Hsp1-Hsp2Cas9-KY
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Hsp1-Hsp2Cas9 (K390A, Y446A)

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAAV-CMV-Hsp1-Hsp2Cas9-Y-P2A-puro

    Plasmid

    #192132

    Purpose

    Expresses highly specific Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

    Depositor

    Yongming Wang

    Article

    Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

    Insert

    Hsp1-Hsp2Cas9-Y
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Hsp1-Hsp2Cas9 (Y446A)

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV/CBA::EGFP-P2A-tC3-FLAG

    Plasmid

    #129477

    Purpose

    AAV2 transfer plasmid for truncated C3 tagged with FLAG with EGFP under control of the CBA promoter

    Depositor

    Robert Gross

    Insert

    FLAG-tagged (C-terminus) truncated (AA173-201) exoenzyme C3 from C. botulinum, P2A, EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBA

    Available since

    Feb. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAc5.1B-lambdaN-HA-DmPop2a-D53AE55A-dsRNAres-V5His6_K

    Plasmid

    #146718

    Purpose

    Insect Expression of DmPop2a-D53AE55A-dsRNAres

    Depositor

    Elisa Izaurralde

    Insert

    DmPop2a-D53AE55A-dsRNAres (Pop2 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    ORF extracted from CG5684-Pa; NM_140281.2, with o…

    Promoter

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. T7-VEE-CFP-e2a-SOX2-p2a-KLF4

    Plasmid

    #193358

    Purpose

    VEE-CFP-SK (self-replicating RNA vector expressing CFP + human SOX2 + KLF4 + PuroR)

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    CFP-e2a-SOX2-p2a-KLF4 (KLF4, SOX2 Human, Aequorea victoria)
    

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCXLE-mCherry-t2a-SOX2AV-p2a-KLF4

    Plasmid

    #193295

    Purpose

    episomal vector expressing mCherry + human SOX2A61V + KLF4

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    mCherry-t2a-SOX2AV-p2a-KLF4 (SOX2, KLF4 Human, Discosoma)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SOX2A61V is a highly cooperative point mutant of …

    Promoter

    CAG

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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27. pLV hUbC-Cas9-P2A-Puro_ST-sgRNA

    Plasmid

    #188705

    Purpose

    Lentivirus transfer plasmid encoding Cas9, puromycin resistance, and 4 sgRNAs targeting human safe targeting loci

    Depositor

    David Tobin

    Article

    Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

    Insert

    ST sgRNAs
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHR-PGK-eGFP-P2A-CRY2-VPR

    Plasmid

    #192610

    Purpose

    Lentiviral vector for constitutive expression of eGFP-P2A-CRY2-VPR.

    Depositor

    Yingxiao Wang

    Article

    Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

    Insert

    eGFP-P2A-CRY2PHR-VPR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    PGK

    Available since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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29. AttB_ACE2[R.ferrum]-2A-TMPRSS2_IRES_iRFP670-H2A-P2A-PuroR

    Plasmid

    #190078

    Purpose

    express Rhinolophus ferrumequinum ACE2 and human TMPRSS2 with selectable markers iRFP670 and puromycin resistance gene

    Depositor

    Kenneth Matreyek

    Article

    Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

    Insert

    ACE2 (TMPRSS2 Rhinolophus ferrumequinum)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    none

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTR-348 pUC19-tNGFR-P2A-ICOS N

    Plasmid

    #186066

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    ICOS (ICOS Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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