We narrowed to 17,736 results for: EMB

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pCSIIpuro-miRFP670-eDHFR(69K6)

Plasmid

#178852

Purpose

Mammalian expression of miRFP670-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

miRFP670-eDHFR(69K6)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1-alpha

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-mNG-eDHFR(69K6)

Plasmid

#178853

Purpose

Mammalian expression of mNeonGreen-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

mNeonGreen-eDHFR(69K6)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1-alpha

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS-eDHFR(69K6)-iRFP713

Plasmid

#178855

Purpose

Mammalian expression of eDHFR(69K6)-iRFP713

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

eDHFR(69K6)-iRFP713

Expression

Mammalian

Promoter

CAG

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-miRFP670-eDHFR(69K6)

Plasmid

#178859

Purpose

Mammalian expression of miRFP670-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

miRFP670-eDHFR(69K6)

Expression

Mammalian

Promoter

CMV

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLV.U6gLacZ.EFS-NS.H2B-RFP

Plasmid

#170363

Purpose

Negative control plasmid used for Crispr/cas9 based disruption. Expressing nuclear RFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

H2B-RFP

Use

Lentiviral

Promoter

EFS-NS

Available Since

June 27, 2021

Availability

Academic Institutions and Nonprofits only

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pHA841 - hrp-1p::hrp-1HsLCWT

Plasmid

#139198

Purpose

Expresses hrp-1p::hrp-1HsLCWT::hrp-1 3'UTR

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Expression

Worm

Mutation

last exon replaced with correspoding human sequen…

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHA842 - hrp-1p::hrp-1HsLCD290V

Plasmid

#139199

Purpose

Expresses hrp-1p::hrp-1HsLCD290V::hrp-1 3'UTR

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Expression

Worm

Mutation

D290V, last exon replaced with correspoding human…

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHA848 - mec-4p::hrp-1HsLCWTmScarlet

Plasmid

#139202

Purpose

Expresses mScarlet tagged hrp-1HsLCWT in mec-4 neurons

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Expression

Worm

Mutation

mScarlet added between RRMs and LC, last exon rep…

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHA849 - mec-4p::hrp-1HsLCD290VmScarlet

Plasmid

#139203

Purpose

Expresses mScarlet tagged hrp-1HsLCD290V in mec-4 neurons

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Expression

Worm

Mutation

mScarlet added between RRMs and LC, D290V, last e…

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHA845 - hrp-1p::hrp-1HsLCWTmScarlet

Plasmid

#139205

Purpose

Expresses mScarlet tagged hrp-1HsLCWT

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Expression

Worm

Mutation

mScarlet added between RRMs and LC, last exon rep…

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHA846 - hrp-1p::hrp-1HsLCD290VmScarlet

Plasmid

#139206

Purpose

Expresses mScarlet tagged hrp-1HsLCD290V

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Expression

Worm

Mutation

mScarlet added between RRMs and LC, D290V, last e…

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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hnRNPF_PLD

Plasmid

#139113

Purpose

express prion like domain of hnRNPF

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hnRNPF (HNRNPF Human)

Expression

Bacterial

Mutation

365-415

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pBTK110

Plasmid

#110576

Purpose

BTK Type 2 promoter for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

CP6 Promoter with RBS

Use

Synthetic Biology

Available Since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK112

Plasmid

#110577

Purpose

BTK Type 2 promoter for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

CP12b Promoter with RBS

Use

Synthetic Biology

Available Since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK113

Plasmid

#110578

Purpose

BTK Type 2 promoter for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

CP32 Promoter with RBS

Use

Synthetic Biology

Available Since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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KHLH31 pEGFP-N2 (1089)

Plasmid

#62032

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

KHLH31

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
tdEos-Supervillin-45

Plasmid

#57671

Purpose

Localization: Membrane/Actin, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Fang et al Traffic. 2010 Jun;11(6):782-99. doi: 10.

Insert

Supervillin

Tags

tdEos

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pAV10

Plasmid

#63213

Purpose

pUC19 backbone (Amp), contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. NotI or FseI sites for TU digest.

Depositor

Jef Boeke

Article

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn NS34a-msGFP-'S-mito

Plasmid

#158768

Purpose

Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2020 Jun 9;9. pii: 56469. doi: 10

Insert

NS3/4a protease and msGFP

Use

Lentiviral

Mutation

Flexible linkers between the protease and msGFP a…

Availability

Academic Institutions and Nonprofits only

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pEGFP VAMP8

Plasmid

#42311

Depositor

Thierry Galli

Article

Paumet et al J Immunol. 2000 Jun 1;164(11):5850-7.

Insert

VAMP 8 (VAMP8 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2013

Availability

Academic Institutions and Nonprofits only

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pMV_AA050

Plasmid

#216107

Purpose

gDNA expression for Cas9 with ChenF+E trRNA (guide only)

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

gDNA expression for Cas9 with ChenF+E trRNA

Use

CRISPR and Lentiviral; Assembled vector

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-STAT2

Plasmid

#71451

Purpose

3rd generation lentiviral expression of STAT2

Depositor

George Stark

Article

Cheon et al EMBO J. 2013 Oct 16;32(20):2751-63. doi:

Insert

STAT2 (STAT2 Human)

Use

Lentiviral

Tags

FLAG

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDB_181

Plasmid

#216102

Purpose

CRISPRi, EF1a-driven dCas9-KRAB (ZNF10) (Cas only)

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

Cas9 [Sp]

Use

CRISPR and Lentiviral; Assembled vector

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDA_831

Plasmid

#216100

Purpose

CRISPRa, EF1a-driven dCas9-VP64 (Cas only)

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

Cas9 [Sp]

Use

CRISPR and Lentiviral; Assembled vector

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-C1-PLCdelta-PH

Plasmid

#21179

Purpose

encodes a PI(4,5)P2 lipid selective PH domain that can be used as a fluorescent translocation biosensor to monitor changes or local differences in the concentration of plasma membrane PI(4,5)P2 lipids

Depositor

Tobias Meyer

Article

Stauffer et al Curr Biol. 1998 Mar 12. 8(6):343-6.

Insert

PLCdelta (PLCD1 Human)

Tags

GFP

Expression

Mammalian

Mutation

PH domain of human PLC delta, amino acids Met1–Il…

Available Since

July 10, 2009

Availability

Academic Institutions and Nonprofits only

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GfaABC1D-Lck-mCherry-ibARK

Plasmid

#241241

Purpose

Expression of plasma membrane-tethered ibARK in astrocytes

Depositor

Jun Nagai

Article

Dewa et al Nature. 2025 Oct 15. doi: 10.1038/s41586

Has Service

AAV5

Insert

Lck-mCherry-ibARK

Use

AAV

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS-EGFP-SOS1

Plasmid

#178860

Purpose

Mammalian expression of SOS1 fused to EGFP

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

SOS1 (SOS1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CAG

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
FB010

Plasmid

#119708

Purpose

Transcriptional Unit (TU) for hygromicin resistance in fungi obtained by combining FB001+GB0211+FB002 into pDGB3alpha1. According to FungalBraid/GoldenBraid modular DNA assembly for ATMT

Depositor

Jose Marcos

Article

Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

Insert

PtrpC:hph:Ttub

Use

Synthetic Biology

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS-PKCdelta-EGFP

Plasmid

#178856

Purpose

Mammalian expression of PKCδ fused to EGFP

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

PKCδ (PRKCD Human)

Tags

EGFP

Expression

Mammalian

Promoter

CAG

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5FRT/TO-Strep/HA-UBE2QL1

Plasmid

#124665

Purpose

Expresses UBE2QL1 with N-terminal Strep-HA tag in mammalian cells

Depositor

Hemmo Meyer

Article

Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

Insert

UBE2QL1 (UBE2QL1 Human)

Tags

2xStrep/HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CAG-miniSOG-C-Far

Plasmid

#193914

Purpose

Control plasmid that expresses miniSOG targeted to the cell membrane in mammalian cells

Depositor

Xiao Wang

Article

Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

Insert

miniSOG

Use

AAV

Tags

Farnesylation signal sequence and Flag tag

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PP_070_pAAV_CAG_LR-Voltron2-P2A-LR-CheRiff-TS-eYFP-ER2

Plasmid

#203228

Purpose

Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.

Depositor

Adam Cohen

Article

Park et al Nat Commun. 2025 Feb 4;16(1):1333. doi:

Insert

Membrane-localized optopatch for neuronal dendrites

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti6-CENP-A-mCherry

Plasmid

#89767

Purpose

Lentivirus destination vector for stable expression of mCherry-tagged kinetochore marker CENP-A

Depositor

Torsten Wittmann

Article

Pemble et al J Cell Sci. 2017 Apr 15;130(8):1404-1412

Insert

CENP-A (CENPA Human)

Use

Lentiviral

Tags

3xHA and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Tom70 (1-70)-GFP

Plasmid

#127633

Purpose

Mitochondrial targeting sequence of Tom70 fused to GFP

Depositor

Josef Kittler

Article

Covill-Cooke et al EMBO Rep. 2020 Feb 5;21(2):e49865. doi:

Insert

Tom70 (TOMM70 Human)

Tags

eGFP

Expression

Mammalian

Mutation

Only first 70 amino acids

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDB_054

Plasmid

#216096

Purpose

All-in-one, knockout, delivers Cas12a & gRNA

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

Cas12a [EnAs]

Use

CRISPR and Lentiviral; Assembled vector

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
NET5s pEGFP-N2 (1154)

Plasmid

#62034

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

Insert

NET5s (TMEM201 Human)

Tags

EGFP

Expression

Mammalian

Mutation

short form

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
NET5L pEGFP-N2 (1155)

Plasmid

#62035

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

NET5L (TMEM201 Human)

Tags

EGFP

Expression

Mammalian

Mutation

long form

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
AAVS1_Cas9-hGem_gRNA2

Plasmid

#217659

Purpose

expresses Cas9-hGem and guideRNA targeting the human AAVS1 safe harbour locus

Depositor

Daniel Gerlich

Article

Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

Insert

sgRNA2 targeting the human AAVS1 safe harbour locus

Use

CRISPR

Expression

Bacterial and Mammalian

Promoter

U6

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
KRas FAK biosensor

Plasmid

#78301

Purpose

FRET biosensor. Visualization of FAK activity at plasma membrane outside lipid rafts in live cells

Depositor

Yingxiao Wang

Article

Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

Insert

KRas FAK biosensor

Expression

Mammalian

Promoter

CMV

Available Since

June 10, 2016

Availability

Academic Institutions and Nonprofits only
pETM-41-HsPABPC1_J

Plasmid

#146644

Purpose

Bacterial Expression of HsPABPc1

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

HsPABPc1 (PABPC1 Human)

Expression

Bacterial

Mutation

two silent mutations compared to the sequence giv…

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG dCas9-KRAB-2A-EGFP

Plasmid

#92396

Purpose

CAG-driven ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. Contains 2A-EGFP reporter. For targeted enhancer inactivation in chicken embryos.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

dCas9_KRAB_2A_EGFP

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Fyn-Fyn Biosensor

Plasmid

#124655

Purpose

FRET biosensor. Fyn-tagged biosensor targeting to membrane

Depositor

Yingxiao Wang

Article

Ouyang et al ACS Sens. 2019 Jan 25;4(1):76-86. doi: 1

Insert

Synthetic gene containing ECFP-SH2 domain-substrate-linker-YPet

Expression

Mammalian

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSP1

Plasmid

#20060

Depositor

Stephen Sligar

Article

Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

Insert

MSP1 (APOA1 synthetic gene)

Tags

6-His

Expression

Bacterial

Mutation

synthetic gene of deletion mutant (1-43) of human…

Available Since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-pmEKAR4

Plasmid

#174440

Purpose

Plasma membrane-targeted EKAR4.

Depositor

Jin Zhang

Article

Keyes et al Elife. 2020 May 26;9. pii: 57410. doi: 1

Insert

pmEKAR4

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAd/CMV/V5-Clasp2α

Plasmid

#89763

Purpose

Adenovirus destination vector for transient expression of human wild-type EGFP-CLASP2α

Depositor

Torsten Wittmann

Article

Pemble et al J Cell Sci. 2017 Apr 15;130(8):1404-1412

Insert

CLASP2α (CLASP2 Human)

Use

Adenoviral

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
MAC_C_FGFR2

Plasmid

#187751

Purpose

MAC-tagged gene expression of human FGFR2

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

FGFR2 (FGFR2 Human)

Expression

Mammalian

Available Since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-IRAlus-Lin28

Plasmid

#92347

Purpose

Plasmid for expression of GFP gene with a Lin28 gene derived IR Alu element containing 3'UTR.

Depositor

Gordon Carmichael, Ling-Ling Chen

Article

Chen et al EMBO J. 2008 Jun 18;27(12):1694-705. doi

Insert

Lin28 gene derived IR Alu element containing 3'UTR (LIN28A Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pOPINS-USP21 (USP, aa 196-565)

Plasmid

#61585

Purpose

Expresses human USP21 (USP domain) in E. coli.

Depositor

David Komander

Article

Ye et al EMBO Rep. 2011 Apr;12(4):350-7. doi: 10.

Insert

USP21 (Ubiquitin carboxyl-terminal hydrolase 21) (USP21 Human)

Tags

His6-SUMO

Expression

Bacterial

Mutation

Deleted aa 1-195.

Promoter

T7

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-Alu-Lin28

Plasmid

#92346

Purpose

Plasmid for expression of GFP gene with a Lin28 gene derived Alu element containing 3'UTR.

Depositor

Gordon Carmichael, Ling-Ling Chen

Article

Chen et al EMBO J. 2008 Jun 18;27(12):1694-705. doi

Insert

Lin28 gene derived Alu element containing 3'UTR (LIN28A Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAcGP67A-hBAI3-HormR-GAIN

Plasmid

#37848

Depositor

Axel Brunger

Article

Arac et al EMBO J. 2012 Feb 14;31(6):1364-78. doi:

Insert

BAI3 (BAI3 Human)

Use

Baculovirus transfer vectors

Tags

8xHIS

Mutation

HormR and GAIN domains only (aa498–868

Promoter

Polyhedrin

Available Since

July 6, 2012

Availability

Academic Institutions and Nonprofits only

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We narrowed to 17,736 results for: EMB