We narrowed to 5,049 results for: golden gate
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Plasmid#209388PurposeGolden Gate compatible plasmid for TurboID-based proximity labelling experiment in plantaDepositorTypeEmpty backboneExpressionPlantMutationTurboID gene sequence C249AAvailable SinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only
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pEC2483 (pSpy1C)
Plasmid#220248Purposereplicative E. coli-S. pyogenes shuttle plasmid for heterologous gene expressionDepositorTypeEmpty backboneExpressionBacterialAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
DHFR-TEV (integrating)
Plasmid#247218PurposePlasmid for genomic integration of DHFR-TEV into the YORWΔ22 locus of yeast cells.DepositorInsertDHFR-TEV
ExpressionYeastPromoterTEF2Available SinceNov. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T7-TALEN(Sangamo)-Destination
Plasmid#37184PurposeDestination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses homodimeric FokI domains.DepositorTypeEmpty backboneTagsFokI nucleaseExpressionMammalianPromoterCAGAvailable SinceJuly 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPOP-BsmBI
Plasmid#241281PurposeCloning gene libraries from oligonucleotide poolsDepositorTypeEmpty backboneUseOtherAvailable SinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPOP-BbsI
Plasmid#241282PurposeCloning gene libraries from oligonucleotide poolsDepositorTypeEmpty backboneUseOtherAvailable SinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
LbCas12a-gRNA_BbsI_CAM
Plasmid#183222PurposeLbCas12a gRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate AssemblyDepositorInsertLbCas12a guide RNA containing BbsI sites in spacer sequence
UseCRISPRExpressionBacterialAvailable SinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPiggyBacATCC LacZ GCTT loxAttP
Plasmid#173496PurposepiggyBac insect transgenesis destination vector for Golden Gate Cloning assembliesDepositorTypeEmpty backboneExpressionInsectAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRO117
Plasmid#233823PurposeMoClo-YTK; type3; Reporter; mNeonGreenDepositorInsertmNeonGreen
ExpressionYeastAvailable SinceMay 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMH220
Plasmid#122836PurposedCas9 with GreenGate B-C flanking sequencesDepositorInsertNuclease-dead SpCas9 (dCas9), codon-optimised for Arabidopsis
UseGolden gate compatible cloning vectorMutationContains D10A and H840A mutationsAvailable SinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBTK1050
Plasmid#209540PurposeBTK Type 1 homology arm for Golden Gate assemblyDepositorInsertUpstream homology arm for recombination into the S. alvi wkB2 genome w/in the SALWKB2_RS11215/SALWKB2_RS11220 intergenic region
UseSynthetic BiologyAvailable SinceFeb. 9, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBTK1051
Plasmid#209541PurposeBTK Type 5 homology arm for Golden Gate assemblyDepositorInsertRecombination into the S. alvi wkB2 genome within the SALWKB2_RS11215/SALWKB2_RS11220 intergenic region
UseSynthetic BiologyAvailable SinceFeb. 2, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJB158
Plasmid#202601PurposeRP4-based mobilizable plasmid for the IPTG inducible expression of inserts that can be introduced using BsaI-based Golden Gate cloning. Vector has two origins of replication.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ148
Plasmid#69300PurposeGolden Gate recipient and Gateway entry vector; assembly of 8 gRNAsDepositorTypeEmpty backboneUseCRISPRAvailable SinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCBLsgRNA1
Plasmid#199727PurposeGolden Gate entry vector; 1st gRNA under OsU3 promoterDepositorInsertOsU3-sgRNA1 scaffold
ExpressionPlantAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCBLsgRNA2
Plasmid#199728PurposeGolden Gate entry vector; 2nd gRNA under OsU3 promoterDepositorInsertOsU3-sgRNA2 scaffold
ExpressionPlantAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGGF012
Plasmid#48849PurposeProvides a sulfadiazine resistance cassette as GreenGate module.DepositorInsertpMAS::SulfadiazineR::t35S
UseGolden gate compatible cloning vectorMutationinternal BsaI recognition sites in SulfR removed …Available SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPOM033_P7_3"His5
Plasmid#216458Purpose3' homology arm for S. pombe genomic integration. Part type 7 following the YeastToolkit MoClo grammar.DepositorInsert3''his5
UseSynthetic BiologyExpressionYeastMutationRemoval of any BsaI // BsmBI restriction siteAvailable SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM026_P2_pNMT1
Plasmid#216451PurposeRegulatable promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.DepositorInsertPromoter nmt1
UseSynthetic BiologyExpressionYeastMutationRemoval of any BsaI // BsmBI restriction siteAvailable SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM019_P2_pRPL2102
Plasmid#216444PurposeConstitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.DepositorInsertPromoter rpl2102
UseSynthetic BiologyExpressionYeastMutationRemoval of any BsaI // BsmBI restriction siteAvailable SinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM015_P2_pADH1
Plasmid#216440PurposeConstitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.DepositorInsertPromoter adh1
UseSynthetic BiologyExpressionYeastMutationRemoval of any BsaI // BsmBI restriction siteAvailable SinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM013_P2_pENO101
Plasmid#216438PurposeConstitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.DepositorInsertPromoter eno101
UseSynthetic BiologyExpressionYeastMutationRemoval of any BsaI // BsmBI restriction siteAvailable SinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pICSL50005
Plasmid#117536PurposeLevel0 Golden Gate part: c-terminal tag, YFPDepositorInsertc-term tag YFP
UseSynthetic BiologyMutationG65S, L68V, A72S, Y203T, L231HAvailable SinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0020
Plasmid#117531PurposeLevel0 Golden Gate part: CDS, SaCas9DepositorInsertSaCas9 (with stop codon)
UseCRISPRAvailable SinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMH183
Plasmid#122856PurposeMS2-modified guide RNA targeting the FT promoter with GreenGate D-E flanking sequencesDepositorInsertMS2-modified sgRNA targeting the Arabidopsis FT promoter
UseGolden gate compatible cloning vectorAvailable SinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBEVY-4420-mCherry
Plasmid#218828PurposeYeast surface display of an anti-fluorescene scFv and intracellular expression of mCherryDepositorInsert4-4-20 scFv
ExpressionYeastPromoterGAL1-10Available SinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cas12c-sgRNA_BbsI_CAM
Plasmid#183221PurposeCas12c sgRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate AssemblyDepositorInsertCas12c single guide RNA containing BbsI sites in spacer sequence
UseCRISPRExpressionBacterialAvailable SinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
BCJJ339
Plasmid#117501PurposeRPS5a:Cas9:E9 Golden Gate Level 1 Position 2DepositorInsertBpiI:GCAA:RPS5a:Cas9-3(intron):E9:ACTA:BpiI
UseCRISPRExpressionPlantPromoterAtRPS5aAvailable SinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPD151 ZF37i
Plasmid#138778PurposeConstitutive expression of ZF37i under the CMV promoter.DepositorInsertZF37i
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0013
Plasmid#117521PurposeLevel0 Golden Gate part: CDS, SpCas9-pDepositorInsertSpCas9-p (with stop codon)
UseCRISPRAvailable SinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGGF002
Plasmid#48843PurposeProvides a BASTA resistance cassette as GreenGate module.DepositorInsertp35S::BastaR::t35S
UseGolden gate compatible cloning vectorMutationinternal BsaI recognition site in 35S promoter re…Available SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYPQ147
Plasmid#69299PurposeGolden Gate recipient and Gateway entry vector; assembly of 7 gRNAsDepositorTypeEmpty backboneUseCRISPRAvailable SinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCBLsgRNA3
Plasmid#199729PurposeGolden Gate entry vector; 3rd gRNA under TaU3 promoterDepositorInsertTaU3-sgRNA3 scaffold
ExpressionPlantAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCBLsgRNA4
Plasmid#199730PurposeGolden Gate entry vector; 4th gRNA under ZmU6-C1 promoterDepositorInsertZmU6C1-sgRNA4 scaffold
ExpressionPlantAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCBLsgRNA5
Plasmid#199731PurposeGolden Gate entry vector; 5th gRNA under ZmU6-C3 promoterDepositorInsertZmU6C3-sgRNA5 scaffold
ExpressionPlantAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCBLsgRNA6
Plasmid#199732PurposeGolden Gate entry vector; 6th gRNA under OsU6.1 promoterDepositorInsertOsU6.1-sgRNA6 scaffold
ExpressionPlantAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCBLsgRNA7
Plasmid#199733PurposeGolden Gate entry vector; 7th gRNA under OsU6.2 promoterDepositorInsertOsU6.2-sgRNA7 scaffold
ExpressionPlantAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCBLsgRNA8
Plasmid#199734PurposeGolden Gate entry vector; 8th gRNA under TaU6 promoterDepositorInsertTaU6-sgRNA8 scaffold
ExpressionPlantAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
BCJJ358
Plasmid#117505PurposeRPS5a:Cas9:E9 Golden Gate Level 1 Position 2 reverseDepositorInsertBpiI:tagt:RPS5a:Cas9_4:E9:ttgc:BpiI
UseCRISPRExpressionPlantPromoterAtRPS5aAvailable SinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
BCJJ344
Plasmid#117503PurposeUBI10:Cas9:E9 Golden Gate Level 1 Position 2 reverseDepositorInsertBpiI:tagt:UBI10:Cas9-3(intron):E9:ttgc:BpiI
UseCRISPRExpressionPlantPromoterAtUBI10Available SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNM3749
Plasmid#154108PurposeloxP FRT FRT3 RMCE landing site in a Golden Gate SapI GCG ACG assembly vectorDepositorInsertsLoxP-SEC-LoxP
FRT::rpl-28p::GFP::his-58::FRT3
UseCre/Lox; RmceExpressionWormPromoternot applicableAvailable SinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPD355 VPR-FRB (NLS)
Plasmid#138847PurposeConstitutive expression of VPR-FRB under the CMV promoter.DepositorInsertVPR-FRB
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
L0-I18 Ub-Arg GG1-GG2
Plasmid#195899PurposeLevel 0 Ubiquitin-Arg tag, protein degration tag with GG1 GG2 golden gate linkersDepositorInsertUb-Arg tag
UseSynthetic BiologyAvailable SinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
BCJJ340
Plasmid#117502PurposeUBI10:Cas9:E9 Golden Gate Level 1 Position 2DepositorInsertBpiI:GCAA:UBI10:Cas9-3(intron):E9:ACTA:BpiI
UseCRISPRExpressionPlantPromoterAtUBI10Available SinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGGF008
Plasmid#48848PurposeProvides a BASTA resistance cassette as GreenGate module.DepositorInsertpNOS::BastaR::tNOS
UseGolden gate compatible cloning vectorMutationchi sequence in BastaR removed by silent substitu…Available SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
L0-I39 PhiC31-Intein GG1-GG4
Plasmid#195905PurposeLevel 0 PhiC31-intein(DnaE-Npu) with GG1 and GG4 golden gate linkersDepositorInsertPhiC31-intein
UseSynthetic BiologyAvailable SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
L0-I40 Intein-PhiC31 GG1-GG4
Plasmid#195906PurposeLevel 0 Intein(DnaE-Ssp)-PhiC31 with GG1 and GG4 golden gate linkersDepositorInsertIntein-PhiC31
UseSynthetic BiologyAvailable SinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLY134
Plasmid#130962PurposeEngineered sgRNA-LEB5 generator including Anderson promoter J23100 for golden gate assemblyDepositorInsertsgRNA-LEB5
UseSynthetic BiologyExpressionBacterialPromoterAnderson promoter J23100Available SinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0058
Plasmid#117539PurposeLevel1 Golden Gate Cassette: sgRNA cassette for SaCas9, targeting NbPDS gene in N. benthamianaDepositorInsertsgRNA (SaCas9)
ExpressionPlantAvailable SinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZA55
Plasmid#174768PurposeEntry clone for Golden Gate assemblyDepositorInsertGG_NbZAR1syn-L17E without STOP codon
UseUnspecifiedMutationL17EAvailable SinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only