Skip to main content
Addgene
Showing: 1081 - 1100 of 24604 results

  4. pFastBacMam5_N_Flag_His8_eGFP_3C

    Plasmid
    #178816
    Purpose
    Baculovirus gene transfer into Mammalian cells to product recombinant proteins.
    Depositor
    Kazuhiro Abe
    Type
    Empty backbone
    Use
    Tags
    Flag, His8, eGFP, 3C
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    178816-plasmid-map-sequence-id-351927

    Enlarge

  10. PX458_3XHA_den1FnCas9_KRAB_T2A_eGFP

    Plasmid
    #200910
    Purpose
    3XHA tagged dead en1Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expression
    Depositor
    Debojyoti Chakraborty
    Insert
    3XHA_den1FnCas9_KRAB_eGFP
    Use
    CRISPR
    Tags
    3xHA-SV40NLS and Nucleoplasmin NLS-KRAB-T2A-EGFP
    Expression
    Mammalian
    Mutation
    Changed Glutamic Acid to Arginine at 1369 position
    Promoter
    CBH
    Available since
    Sept. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    200910-plasmid-map-sequence-id-407164

    Enlarge

  11. sfGFP-ER-3

    Plasmid
    #56482
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 485, Emission: 507
    Depositor
    Michael Davidson
    Insert
    ER (CALR Human)
    Use
    Tags
    sfGFP
    Expression
    Mammalian
    Mutation
    ER Signal Peptide
    Promoter
    CMV
    Available since
    Jan. 13, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  12. INT_Cargo_PgyrA_gfp

    Plasmid
    #212138
    Purpose
    Cargo plasmid for integrating PgyrA expression cassette of green fluorescent protein in genome.Plasmid can be removed by incubating cells at 37 C.
    Depositor
    Kang Zhou
    Insert
    green fluorescent protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PgyrA
    Available since
    Jan. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    212138-plasmid-map-sequence-id-420768

    Enlarge

  13. roGFP_iE_C147S_pQE30

    Plasmid
    #48631
    Purpose
    Bacterial expression vector encoding for the 6XHis tagged insensitive variant of fluorescent redox probe roGFPiE with Cysteine 147 to Serine mutation (roGFP C147S, E147b, C204)
    Depositor
    David Ron
    Article
    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.
    Insert
    roGFP1
    Use
    Tags
    HisTag
    Expression
    Bacterial
    Mutation
    insertion E147B, C147S
    Promoter
    Available since
    Oct. 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    48631-plasmid-map-sequence-id-70379

    Enlarge

  15. KIF3A560GFP

    Plasmid
    #129769
    Purpose
    Expresses mouse Kinesin-2 KIF3A head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP tagged
    Depositor
    William Hancock
    Insert
    KIF3A (Kif3a Mouse)
    Use
    Tags
    GFP and His6
    Expression
    Bacterial
    Mutation
    Truncation at 359, fused to kinesin-1 starting Al…
    Promoter
    Available since
    Nov. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    129769-plasmid-map-sequence-id-263530

    Enlarge

  17. KSP_K560GFP

    Plasmid
    #129770
    Purpose
    Expresses Xenopus Kinesin-5 KSP head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP tagged
    Depositor
    William Hancock
    Insert
    KSP (kif11.L Frog)
    Use
    Tags
    GFP and His6
    Expression
    Bacterial
    Mutation
    Truncation at 367, fused to kinesin-1 starting Al…
    Promoter
    Available since
    Nov. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    129770-plasmid-map-sequence-id-263613

    Enlarge

  20. SIN40C.TRE.MCS.IRES.dTomato.PGK.sfGFP.P2A.Tet3G

    Plasmid
    #169283
    Purpose
    Doxycycline-inducible overexpression of cDNA with dTomato as a reporter fluorescent protein
    Depositor
    Dirk Heckl
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    169283-plasmid-map-sequence-id-337924

    Enlarge

Showing: 1081 - 1100 of 24604 results