170,342 results
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Plasmid#64867PurposeThis plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the engineered shh10 capsid protein.DepositorInsertshh10 cap
UseAAVMutationI319V, N451D, D532NAvailable SinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSBbi 41BBL CD86 HLA-A201
Plasmid#183248PurposeExpression of human 41BBL, CD86 and HLA-A201 in a Sleeping Beauty transposon vector for use in artificial antigen presenting cellsDepositorInsertsExpressionMammalianAvailable SinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMD2.G-VSV-G-mut
Plasmid#196508PurposeVSV-G mutant (K47Q, R354A)DepositorInsertVSV-G
TagsNAExpressionMammalianMutationK47Q, R354APromoterCMVAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
R777-E286 Hs.ROCK2-nostop
Plasmid#70570PurposeGateway ORF clone of human ROCK2 [NM_004850.3] without stop codon (for C-terminal fusions)DepositorInsertROCK2 (ROCK2 Human)
UseGateway entry cloneAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPTK004-2-pTPI1
Plasmid#84963PurposeType 2, pTPI1, Triose phosphate isomerase 1 promoterDepositorInsertTriose phosphate isomerase 1 promoter
UseSynthetic BiologyExpressionYeastPromoterNonAvailable SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
SpRY Cas9
Plasmid#188491PurposeExpresses FLAG tagged SpRY Cas9DepositorInsertCas9
UseCRISPR and LentiviralExpressionMammalianMutationA61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…PromoterEF1aAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTSlb-R756K
Plasmid#60731PurposeExpresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations R756SDepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…Available SinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-anti-PDL1(KN035) PAGER(TF)
Plasmid#230003PurposeExpresses anti-PDL1 PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)DepositorInsertIL2SP-Aro6-KN035-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
UseAAVExpressionMammalianMutationV360A/R361A on KORDPromoterCMVAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
1a. pETM11-SUMO-SNCA-GFP
Plasmid#107292PurposePlasmid for the expression of the construct alpha-Synuclein-GFP, which contains N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubilityDepositorAvailable SinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBV-Luc
Plasmid#16539Purposeluciferase reporter plasmid with very low basal activityDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLuciferaseTagsLuciferaseExpressionMammalianAvailable SinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFGM1
Plasmid#64949Purposesource of GmR FRT cassetteDepositorInsertFRT-GmR-FRT
ExpressionBacterialAvailable SinceNov. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-SAFB-NLS-3XFLAG-V5
Plasmid#196089PurposeExpresses mouse SAFB in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tagDepositorInsertSAFB (Safb Mouse)
TagsC-terminal 3xFLAG and C-terminal V5ExpressionBacterial and MammalianPromoterCAGGSAvailable SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
MSCV-mir-17-92
Plasmid#64100Purposeexpresses mir-17-92DepositorAvailable SinceJune 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
Notch3 intracellular domain-pcw107-V5
Plasmid#64623Purposewhen used to produce lentivirus, express physiological levels of insertDepositorAvailable SinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDae069 (eMutaT7[PmCDA1] with optimized RBS)
Plasmid#187621PurposeExpresses eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coliDepositorInsertPmCDA1-T7RNAP
ExpressionBacterialAvailable SinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Klf4
Plasmid#13370PurposeRetroviral expression of mouse Klf4DepositorAvailable SinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pv6_MCPH1_BRCT W706RW815R
Plasmid#250355PurposeExpresses mutant tandem BRCT domain from MCPH1 fused to eGFP. Serves as control.DepositorInsertMCPH1 tandem BRCT
TagsNLS, eGFP and biotintag, TEV cleavageExpressionMammalianMutationW706R and W815RPromoterCAGGSAvailable SinceFeb. 10, 2026AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EGFP-HOTag3
Plasmid#225677PurposeUsed to generate stable cell lines that express EGFP-HOTag3. This plasmid serves as a control for pLVX-PERK(LD)-EGFP-HOTag3.DepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceFeb. 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
pEcLeuRSH6
Plasmid#177591PurposeExpresses His tagged LeuRS in Escherichia coli cellsDepositorInsertLeucine tRNA synthetase
TagsHexahistidineExpressionBacterialAvailable SinceFeb. 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
pPig_8X-TOPFLash-tdIRFP_Puro
Plasmid#249713PurposeTopFlash reporter with puromycin. Used as a live readout of downstream Wnt gene transcription.DepositorInsertstdiRFP
T2A-PuroR
ExpressionMammalianPromoterTopFlash and TopFlash, with 7x TCF/LEF motifAvailable SinceJan. 14, 2026AvailabilityAcademic Institutions and Nonprofits only