159,481 results

1. pspCas9-HF-tetra-com-vector

   Plasmid

   #132555

   Purpose

   Vector plasmid expressing high fidelity spCas9 (R691A) and sgRNA scaffold with com replacing the Tetraloop.

   Depositor

   Baisong Lu

   Article

   Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

   Inserts

   High fidelity Cas9
   sgRNA scaffold with com replacing the Tetraloop
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19-Hph

   Plasmid

   #188741

   Purpose

   pUC19 plasmid (MCS intact) with Hygromycin selection marker

   Depositor

   Michael Bromley

   Article

   Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

   Insert

   Hygromycin
   

   Use

   Filamentous fungal expression

   Tags

   Expression

   Mutation

   Promoter

   gpdA

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. CRY2high-mCherry

   Plasmid

   #104063

   Purpose

   Expresses fusion of CRY2high mutant (CRY2PHR E490R) with mCherry

   Depositor

   Bianxiao Cui

   Article

   Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

   Insert

   CRY2high-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   CRY2PHR E490R

   Promoter

   CMV

   Available since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV5-hPPT1

   Plasmid

   #85815

   Purpose

   Expression of hPPT in mammalian cells

   Depositor

   Sandra Hofmann

   Article

   Lu et al Mol Genet Metab. 2010 Apr;99(4):374-8. d

   Insert

   human PPT1 (PPT1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLX304-IRES-Td

   Plasmid

   #176296

   Purpose

   pLX304 backbone with an IRES-Tdtomato cassette subcloned into the NheI-AgeI sites

   Depositor

   William Kaelin

   Article

   Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSDM052

   Plasmid

   #194341

   Purpose

   pUC mini-Tn7 vector expressing LseCas13a

   Depositor

   Joseph Bondy-Denomy

   Article

   Mendoza et al Nature. 2020 Jan;577(7789):244-248. doi:

   Insert

   Cas13a(Lse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-MS2-HA-HsRoquin1_AE

   Plasmid

   #148611

   Purpose

   Mammalian Expression of HsRoquin1

   Depositor

   Elisa Izaurralde

   Article

   Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

   Insert

   HsRoquin1 (RC3H1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCIneo-lambdaN-HA-HsEDC4

   Plasmid

   #66597

   Purpose

   Expresses lambdaN-HA-tagged EDC4 in mammalian cells

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

   Insert

   EDC4 (EDC4 Human)
   

   Use

   Tags

   lambdaN-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFast-FT-N1

   Plasmid

   #31910

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

   Insert

   Fast-FT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   M18V/E34K/K69R/L84W/S149T/A224S compared to mCher…

   Promoter

   CMV

   Available since

   Sept. 12, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL3-MTS-ccdb-DddA(G1397C)-UGI-Puro

    Plasmid

    #173230

    Purpose

    One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

    Depositor

    Bin Shen

    Article

    Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL3-MTS-ccdb-DddA(G1397N)-UGI-Puro

    Plasmid

    #173231

    Purpose

    One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

    Depositor

    Bin Shen

    Article

    Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. loxP-Hygro-loxP-HA-mStayGold-Rab11 HR

    Plasmid

    #229678

    Purpose

    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with monomeric StayGold and an HA epitope tag. Contains a hygromycin resistance cassette for selection of edited cells

    Depositor

    Francesca Bottanelli

    Article

    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

    Insert

    genomic sequence Rab11 locus (RAB11A Human)
    

    Use

    CRISPR

    Tags

    monomeric StayGold, HA

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-T7-AcrIIC3-NLS(SV40) (KAC209)

    Plasmid

    #134341

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIC3 with C-terminal NLS (SV40)

    Depositor

    Benjamin Kleinstiver, Joseph Bondy-Denomy

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized AcrIIC3
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-hSTING (V155M)

    Plasmid

    #214161

    Purpose

    Transient expression of gain-of-function STING mutant

    Depositor

    Nan Yan

    Article

    Dobbs et al Cell Host Microbe. 2015 Aug 12;18(2):157

    Insert

    STING (V155M) (STING1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    V155M

    Promoter

    CMV

    Available since

    March 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pV238-vPE

    Plasmid

    #225258

    Purpose

    Mammalian expression of vPE

    Depositor

    Vikash Chauhan, Robert Langer, Phil Sharp

    Article

    Chauhan et al bioRxiv 2024.08.02.606370

    Insert

    vPE
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    R221K K848A H982A N1317R

    Promoter

    CMV

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV.CAG-FLEX.SF-iGluSnFR.A184S

    Plasmid

    #106186

    Purpose

    Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-iGluSnFR.A184S
    

    Use

    AAV

    Tags

    Expression

    Mutation

    GltI: A184S

    Promoter

    CAG

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT7_zntR

    Plasmid

    #216324

    Purpose

    T7 promoter driving expression of zntR

    Depositor

    Mark Styczynski

    Article

    McNerney et al Sci Adv. 2019 Sep 25;5(9):eaax4473. doi:

    Insert

    Zinc-responsive activator
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. yo-msGFP2

    Plasmid

    #160471

    Purpose

    Encodes a yeast codon optimized version of msGFP2

    Depositor

    Benjamin Glick

    Article

    Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

    Insert

    yo-msGFP2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEXP5-NT/6xHis mCherry F30 2xdBroccoli

    Plasmid

    #169233

    Purpose

    Constitutive T7 RNAP-mediated expression of 6xHis-mCherry with 2xd Broccoli RNA aptamer.

    Depositor

    Dora Tang

    Article

    Gonzales et al ACS Synth Biol. 2022 Jan 21;11(1):205-21

    Insert

    6xHis mCherry F30-2xdBroccoli
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Dec. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. p0521s

    Plasmid

    #62862

    Purpose

    Episome vector for Phaeodactylum tricornutum (pCC1BAC-based)

    Depositor

    Hamilton Smith

    Article

    Karas et al Nat Commun. 2015 Apr 21;6:6925. doi: 10.

    Type

    Empty backbone

    Use

    Synthetic Biology; Episomal vector for phaeodacty…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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