162,160 results

1. pcDNA3-Ii31

   Plasmid

   #64598

   Purpose

   Expression of invariant chain as model antigen for study of MHC class II presentation

   Depositor

   Sandra Diebold, Martin Zenke

   Article

   Diebold et al Gene Ther. 2001 Mar;8(6):487-93.

   Insert

   MHC II Invariant Chain
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-TRE-OKSM

   Plasmid

   #204498

   Purpose

   Expresses Oct4, Klf4, Sox2 and c-Myc by tet-on promoter in mammalian cells

   Depositor

   Wataru Fujii

   Article

   Matsuya et al Sci Rep. 2024 Feb 8;14(1):3204. doi: 10.

   Inserts

   OCT4 (POU5F1 Human)
   KLF4 (KLF4 Human)
   SOX2 (SOX2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSB700 lenti gRNA Puro

   Plasmid

   #167911

   Purpose

   Lentiviral vector for expressing U6 sgRNA cloning plasmid

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSin_EF1a_ERT2_CreN59_nMag_P2A_mCh_IRES_PuroR

   Plasmid

   #192636

   Purpose

   Expresses ERT2-CreN59-nMag-NLS with mCherry marker.

   Depositor

   Yingxiao Wang

   Article

   Allen et al ACS Synth Biol. 2019 Oct 18;8(10):2359-2

   Insert

   ERT2_CreN59_nMag_NLS_P2A_mCh_IRES_PuroR
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEL035

   Plasmid

   #137903

   Purpose

   STU2.0 SpyCas9(D10A)-PmCDA1 for plant genome base editing

   Depositor

   Yong Zhang

   Article

   Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFRT_TO_FlagHA_USP10

   Plasmid

   #127102

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

   Insert

   USP10 (USP10 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. LifeAct-mGarnet

   Plasmid

   #104307

   Purpose

   codes for a fusion of fluorescent protein mGarnet and lifeact that targets F-actin in live cells

   Depositor

   Ulrich Nienhaus

   Article

   Hense et al Sci Rep. 2015 Dec 9;5:18006. doi: 10.103

   Insert

   LifeAct-mGarnet
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDB5319

   Plasmid

   #204829

   Purpose

   An integrating plasmid containing both the inducible enotetSW1 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.

   Depositor

   Li-Lin Du

   Article

   Lyu et al J Cell Sci. 2024 Nov 1;137(21):jcs263404

   Inserts

   tetR
   mECitrine
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   CMV promoter and enotetSW1 promoter

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV:ITR-U6-sgRNA(LacZ)-pCBh-Cre-WPRE-hGHpA-ITR

   Plasmid

   #60228

   Purpose

   Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA control targeting LacZ. AAV backbone.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   sgRNA
   Cre recombinase
   

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   Cre-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   Nov. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMVd1-NLS-GAL4-iLight2-VP16-T2A-mTagBFP2

    Plasmid

    #219548

    Purpose

    NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2 construct expression in mammalian cells.

    Depositor

    Vladislav Verkhusha

    Article

    Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

    Insert

    NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The (SAGG)x4 linker between GAL4(1-65) and the PC…

    Promoter

    CMVd1

    Available since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMTS-HA-GFP11

    Plasmid

    #177725

    Purpose

    Eleventh β-strand of GFP (GFP11) fused to a mitochondria signal sequence for mitochondrial targeting, HA tagged

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    MTS-HA-GFP11
    

    Use

    Tags

    GFP11 and HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHMGWA-Pa14Csy4H29A

    Plasmid

    #41092

    Depositor

    Jennifer Doudna

    Article

    Haurwitz et al Science. 2010 Sep 10;329(5997):1355-8.

    Insert

    Csy4
    

    Use

    Tags

    His6-MBP

    Expression

    Mutation

    His29Ala mmutation to abolish ribonuclease activi…

    Promoter

    T7

    Available since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA

    Plasmid

    #61592

    Purpose

    A human codon-optimized Cas9 from Staphylococcus aureus (SaCas9).

    Depositor

    Feng Zhang

    Article

    Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

    Has Service

    AAV6

    Insert

    hSaCas9
    

    Use

    AAV and CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. lentiCRISPR_DKO

    Plasmid

    #183192

    Purpose

    Plasmid with Cas9, two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen

    Depositor

    Lang Li

    Article

    Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6, mU6

    Available since

    Aug. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti- V6.3 lox-dsRED-stop-lox-eGFP-blas

    Plasmid

    #106171

    Purpose

    cre mediated red to green shift

    Depositor

    Ewa Snaar-Jagalska

    Article

    Heitzer et al Sci Rep. 2019 Mar 11;9(1):4096. doi: 10.

    Inserts

    dsRED-express2
    eGFP
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti CMVtight eGFP Puro (w771-1)

    Plasmid

    #26431

    Purpose

    2nd generation lentiviral vector

    Depositor

    Eric Campeau

    Insert

    Enhanced Green Fluorescent Protein
    

    Use

    Lentiviral; Tet-on advanced vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcdna3.1 hciap1

    Plasmid

    #8311

    Depositor

    Jon Ashwell

    Article

    Li et al Nature 2002 Mar 21;416(6878):345-7.

    Insert

    cIAP1 (BIRC2 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. GRB2-V5

    Plasmid

    #139631

    Purpose

    V5-His-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

    Insert

    GRB2 (GRB2 Human)
    

    Use

    Tags

    3xV5-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pWZ853

    Plasmid

    #208115

    Purpose

    mSwAP-In marker cassette 2 with 5’PB-mNeongreen-HPRT1-BSD

    Depositor

    Jef Boeke

    Article

    Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

    Insert

    mNeonGreen-HPRT1-BSD
    

    Use

    Tags

    T2A

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLP-PIGA (pLP140)

    Plasmid

    #168461

    Purpose

    Big-IN Landing Pad plasmid encoding pEF1a-driven mScarlet-P2A-CreERT2-P2A-PuorR-P2A-hmPIGA expression cassette flanked by heterotypic LoxM/LoxP sites and BsaI homology arms cloning sites.

    Depositor

    Jef Boeke, Matthew Maurano

    Article

    Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

    Type

    Empty backbone

    Use

    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge