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161,813 results

Showing: 1101 - 1120 of 161813 results

  2. pYPQAT31

    Plasmid
    #223403
    Purpose
    T-DNA vector for SpCas9-D10A based A-to-G base editing for dicot plants; NGG PAM; SpCas9D10A-ABE was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    AtUBQ10-ecTadA8e-SpCas9-D10A-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223403-plasmid-map-sequence-id-454164

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  7. kiCAP-AAV-BI30

    Plasmid
    #183749
    Purpose
    AAV-BI30 Rep-Cap plasmid for production of AAV-BI30, a capsid with tropism for CNS endothelial cells.
    Depositor
    Ben Deverman
    Article
    Krolak et al Nat Cardiovasc Res. 2022 Apr;1(4):389-40
    Insert
    AAV9 modified with 7mer insertion between amino acids 588 and 589 of VP1
    Use
    AAV
    Tags
    Expression
    Mutation
    K449R, and NNSTRGG inserted between 588 and 589 o…
    Promoter
    p41
    Available since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    183749-plasmid-map-sequence-id-442496

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  8. lenti sgRNA(MS2)_puro optimized backbone

    Plasmid
    #73797
    Purpose
    optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and EF1A
    Available since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73797-plasmid-map-sequence-id-196924

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  14. pcDNA3/F-tractin=mStayGold

    Plasmid
    #212019
    Purpose
    For filamentous actin labeling. Alternatively, the F-tractin gene can be replaced with a target molecule gene for C-terminal tagging with mStayGold through a linker [(GGGGS)3] (denoted as ‘=’).
    Depositor
    Atsushi Miyawaki
    Article
    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s
    Insert
    F-tractin
    Use
    Tags
    mStayGold
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    212019-plasmid-map-sequence-id-419223

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  16. mClY-N1

    Plasmid
    #90457
    Purpose
    A YFP-based chloride sensor with enhanced chloride sensitivity, photostability and reduced pH interference. The sequence of mClY is EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206K.
    Depositor
    Joseph Santos-Sacchi
    Article
    Zhong et al PLoS One. 2014 Jun 5;9(6):e99095. doi: 1
    Insert
    mClY-N1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206…
    Promoter
    Available since
    May 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    90457-plasmid-map-sequence-id-178580

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  17. pEcCas

    Plasmid
    #73227
    Purpose
    Constitutive expression of cas9 and inducible expression of lambda RED and sgRNA
    Depositor
    Sheng Yang
    Article
    Li et al Acta Biochim Biophys Sin (Shanghai). 202
    Inserts
    Cas9
    sgRNA
    Use
    CRISPR; Red recombinase
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    native promoter
    Available since
    June 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    73227-plasmid-map-sequence-id-419024

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Showing: 1101 - 1120 of 161813 results