We narrowed to 119,908 results for: CIL

1. pcDNA3-ER-AKAR4

   Plasmid

   #64733

   Purpose

   Endoplasmic reticulum (ER)-targeted FRET-based PKA activity reporter; targeted to cytosolic face of ER membrane.

   Depositor

   Jin Zhang

   Article

   Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

   Insert

   AKAR4
   

   Tags

   Cerulean, N-terminal 27 amino acids from CYP450, …

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMRX-IPU-FLAG-NEK9

   Plasmid

   #168269

   Purpose

   Expresses NEK9 in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi:

   Insert

   NEK9 (NEK9 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCherry-Dyskerin

   Plasmid

   #207577

   Purpose

   N-terminal mCherry-tag using SalI/EcoRI

   Depositor

   Jens Schmidt

   Article

   Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

   Insert

   generated by restriction cloning of gene into a modified pBABE-puro vector containing the N-terminal mCherrytag using SalI/EcoRI
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. BS-TRP1-TetOff(7repeats)-GFP-Pho8

   Plasmid

   #207011

   Purpose

   Tet-Off controlled GFP-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   Pho8
   

   Tags

   GFP

   Expression

   Yeast

   Available Since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-T7-AcrIIA4-NLS(SV40) (KAC200)

   Plasmid

   #133801

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIA4 with C-terminal NLS (SV40)

   Depositor

   Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized AcrIIA4
   

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   CMV and T7

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-T7-AcrIIA5-NLS(SV40) (KAC203)

   Plasmid

   #133802

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIA5 with C-terminal NLS (SV40)

   Depositor

   Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized AcrIIA5
   

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   CMV and T7

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBS MCK promoter

   Plasmid

   #12528

   Depositor

   Ronald Kahn

   Article

   Bruning et al Mol Cell. 1998 Nov . 2(5):559-69.

   Insert

   MCK promoter (Ckm Mouse)
   

   Expression

   Mammalian

   Available Since

   Aug. 18, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCALNL-NaChBac

   Plasmid

   #125580

   Purpose

   Cre-dependent expression of NaChBac (FLAG-tagged) in mammalian cells.

   Depositor

   Takeshi Imai

   Article

   Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

   Insert

   NaChBac
   

   Use

   Cre/Lox

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. 3xFLAG-Halo-Dyskerin

   Plasmid

   #207578

   Purpose

   CRISPR-Cas9 genome editing encoding a FLAG-HaloTag at the endogenous dyskerin locus

   Depositor

   Jens Schmidt

   Article

   Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

   Insert

   HaloTag DKC1
   

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFudio-Cpg15-IRES2-TdTomatoW

    Plasmid

    #134309

    Purpose

    Expresses cpg15 and TdTomato upon coexpression with Cre recombinase

    Depositor

    Elly Nedivi

    Article

    Subramanian et al Cell Rep. 2019 Aug 6;28(6):1584-1595.e5.

    Inserts

    cpg15 (Nrn1 Mouse, Rat)
    TdTomato
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Promoter

    UbC

    Available Since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL3

    Plasmid

    #48743

    Purpose

    Expression of luciferase driven by truncated mPer2 promoter region (bases -1128 to +1060 with respect to transcription start site at +1)

    Depositor

    Joseph Takahashi

    Article

    Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10

    Insert

    truncated mPer2 promoter/enhancer region (-1128 to +1060)
    

    Use

    Luciferase

    Mutation

    truncated promoter region (see pGL6)

    Available Since

    Dec. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pcpBpaRS

    Plasmid

    #196484

    Purpose

    To express benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into benzoylphenylalanine.

    Depositor

    Kensaku Sakamoto

    Article

    Hino et al Methods Mol Biol. 2012;794:215-28. doi:

    Insert

    benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS), amber suppressor tRNA
    

    Expression

    Mammalian

    Promoter

    CMV (synthetase), Pol III tRNA promoter (tRNA)

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pALOD4 non-binding mutant

    Plasmid

    #111027

    Purpose

    Expresses non-binding mutant version of p-ALOD4, has six inactivating mutations (G501A, T502A, T503A, L504A, Y505A, and P506A)

    Depositor

    Arun Radhakrishnan

    Article

    Gay et al Biophys J. 2015 Mar 24;108(6):1459-69. d

    Insert

    Domain 4 of Anthrolysin O
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Mutations: S404C, C472A, G501A, T502A, T503A, L5…

    Promoter

    T7

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBITE-EGFRvIII-F271-OKT3

    Plasmid

    #190682

    Purpose

    Encodes an EGFRvIII-CD3 BITE protein (F271 clone)

    Depositor

    Scott McComb

    Article

    Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

    Insert

    Anti-EGFRvIII-scFv-F271-modularized-OKT3-HisTag
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA-FLAG-Bbs4

    Plasmid

    #128868

    Purpose

    For mammalian expression of mouse Bbs4

    Depositor

    Aimin Liu

    Article

    Ye et al Proc Natl Acad Sci U S A. 2014 Feb 11;11

    Insert

    Bbs4 (Bbs4 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. PADH1-TIR1-9myc-URA3

    Plasmid

    #207016

    Purpose

    Expresses TIR1-9Myc. TIR1 mediates auxin-induced ubiquitination of auxin-inducible degron (AID).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    TIR1
    

    Tags

    9xMyc

    Expression

    Yeast

    Promoter

    pADH1

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. MSCVhygro-F-Ezh2-F667I

    Plasmid

    #24927

    Depositor

    Kai Ge

    Article

    Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

    Insert

    Ezh2 (EZH2 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Phenylanaline(F)667 mutated to Isoleucine(I)

    Available Since

    July 15, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3-ER-CaNAR2

    Plasmid

    #64732

    Purpose

    Endoplasmic reticulum (ER)-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of ER membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CYP450-CaNAR2
    

    Tags

    Cerulean3, N-terminal 27 amino acids from CYP450,…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEF5/FRT-DEST EVC2-Flag

    Plasmid

    #41005

    Depositor

    Rajat Rohatgi

    Article

    Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

    Insert

    Ellis van Creveld syndrome 2 (EVC2 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Nov. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

20. BS-TRP1-TetOff(7 repeats)-mRuby3-Pho8

    Plasmid

    #207031

    Purpose

    Tet-Off controlled mRuby3-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    Pho8
    

    Tags

    mRuby3

    Expression

    Yeast

    Available Since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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