We narrowed to 117,326 results for: CIL

1. 3xFLAG-Halo-Dyskerin

   Plasmid

   #207578

   Purpose

   CRISPR-Cas9 genome editing encoding a FLAG-HaloTag at the endogenous dyskerin locus

   Depositor

   Jens Schmidt

   Article

   Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

   Insert

   HaloTag DKC1
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGL3

   Plasmid

   #48743

   Purpose

   Expression of luciferase driven by truncated mPer2 promoter region (bases -1128 to +1060 with respect to transcription start site at +1)

   Depositor

   Joseph Takahashi

   Article

   Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10

   Insert

   truncated mPer2 promoter/enhancer region (-1128 to +1060)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   truncated promoter region (see pGL6)

   Promoter

   Available since

   Dec. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pCMV-T7-AcrIIA4-NLS(SV40) (KAC200)

   Plasmid

   #133801

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIA4 with C-terminal NLS (SV40)

   Depositor

   Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized AcrIIA4
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   CMV and T7

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMRX-IPU-FLAG-NEK9

   Plasmid

   #168269

   Purpose

   Expresses NEK9 in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi:

   Insert

   NEK9 (NEK9 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGEX6P1-mClover3-anti-rabbit-IgG(Fc)-nanobody

   Plasmid

   #138129

   Purpose

   Expresses GST-tagged mClover3-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

   Insert

   mClover3-anti-rabbit-IgG(Fc)-nanobody
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. GFP-noPS

   Plasmid

   #177943

   Purpose

   Transient mammalian expression of eGFP. Lacks packaging sequence and is used as a negative control transfer plasmid for SARS-CoV-2 virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   eGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PADH1-TIR1-9myc-URA3

   Plasmid

   #207016

   Purpose

   Expresses TIR1-9Myc. TIR1 mediates auxin-induced ubiquitination of auxin-inducible degron (AID).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   TIR1
   

   Use

   Tags

   9xMyc

   Expression

   Yeast

   Mutation

   Promoter

   pADH1

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. a1Ic-HE3-pcDNA3

   Plasmid

   #45810

   Depositor

   Edward Perez-Reyes

   Article

   Gomora et al Biophys J. 2002 Jul;83(1):229-41.

   Insert

   CACNA1I (CACNA1I Human)
   

   Use

   Making crna for oocyte injections

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-T7-AcrIIA5-NLS(SV40) (KAC203)

   Plasmid

   #133802

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIA5 with C-terminal NLS (SV40)

   Depositor

   Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized AcrIIA5
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   CMV and T7

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAB076i7

    Plasmid

    #79201

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI(RR69)-SH2ABL1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlacZ-opt (OR1); Ppro1

    Available since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA-FLAG-Bbs4

    Plasmid

    #128868

    Purpose

    For mammalian expression of mouse Bbs4

    Depositor

    Aimin Liu

    Article

    Ye et al Proc Natl Acad Sci U S A. 2014 Feb 11;11

    Insert

    Bbs4 (Bbs4 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFudio-Cpg15-IRES2-TdTomatoW

    Plasmid

    #134309

    Purpose

    Expresses cpg15 and TdTomato upon coexpression with Cre recombinase

    Depositor

    Elly Nedivi

    Article

    Subramanian et al Cell Rep. 2019 Aug 6;28(6):1584-1595.e5.

    Inserts

    cpg15 (Nrn1 Rat, Mouse)
    TdTomato
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UbC

    Available since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcpBpaRS

    Plasmid

    #196484

    Purpose

    To express benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into benzoylphenylalanine.

    Depositor

    Kensaku Sakamoto

    Article

    Hino et al Methods Mol Biol. 2012;794:215-28. doi:

    Insert

    benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS), amber suppressor tRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (synthetase), Pol III tRNA promoter (tRNA)

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. BS-TRP1-TetOff(7 repeats)-mRuby3-Pho8

    Plasmid

    #207031

    Purpose

    Tet-Off controlled mRuby3-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    Pho8
    

    Use

    Tags

    mRuby3

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKAR2-Br512_Mut235

    Plasmid

    #179278

    Purpose

    Expresses Br512_Mut235 polymerase in E.coli

    Depositor

    Andrew Ellington

    Article

    Paik et al Biochemistry. 2021 Nov 11. doi: 10.1021/

    Insert

    Engineered Bst DNA Polymerase I Large Fragment
    

    Use

    Tags

    8x His tag; Villin Headpiece 47 amino acid (HP47)…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Halo-TCAB1 HRD

    Plasmid

    #207535

    Purpose

    Homologous recombination donor to insert HaloTag at the N-terminus of the endogenous TCAB1 locus.

    Depositor

    Jens Schmidt

    Article

    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

    Insert

    HaloTag with internal PuroR cassette flanked by human TCAB1 locus sequences
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Endogenous

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBS MCK promoter

    Plasmid

    #12528

    Depositor

    Ronald Kahn

    Article

    Bruning et al Mol Cell. 1998 Nov . 2(5):559-69.

    Insert

    MCK promoter (Ckm Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEF5/FRT-DEST EVC2-Flag

    Plasmid

    #41005

    Depositor

    Rajat Rohatgi

    Article

    Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

    Insert

    Ellis van Creveld syndrome 2 (EVC2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pBluescript-IC3-FLAG-Tev-ZZ

    Plasmid

    #170311

    Purpose

    Plasmid for endogenous C-terminal tagging of the Tetrahymena thermophila Outer Dynein Arm intermediate chain 3 with FLAG and ZZ tags.

    Depositor

    Andrew Carter

    Article

    Mali et al Science. 2021 Feb 26;371(6532):910-916.

    Insert

    Outer Dynein Arm Intermediate Chain 3
    

    Use

    Tags

    FLAG, ZZ

    Expression

    Mutation

    Promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBITE-EGFRvIII-F271-OKT3

    Plasmid

    #190682

    Purpose

    Encodes an EGFRvIII-CD3 BITE protein (F271 clone)

    Depositor

    Scott McComb

    Article

    Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

    Insert

    Anti-EGFRvIII-scFv-F271-modularized-OKT3-HisTag
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge