We narrowed to 1,922 results for: cas9 expression vector

1. pWR63(YUM1)

   Plasmid

   #203336

   Purpose

   episomal Cas9 and YUM1-targeting sgRNA expression vector for S. stipitis

   Depositor

   J. Brian Robertson

   Article

   Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

   Inserts

   TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
   coHPH
   ACT1 terminator (S. stipitis)
   CCW12 promoter (S. stipitis)
   CaCas9
   ADH2 terminator (S. stipitis)
   ARS from S. stipitis chromosome 1
   THD3 promoter (S. stipitis)
   tRNA-sgRNA(SapI)-HDV
   ADH1 terminator (S. stipitis)
   YUM1-targeting sequence
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWR63

   Plasmid

   #203331

   Purpose

   episomal Cas9 and sgRNA expression vector without sgRNA for S. stipitis

   Depositor

   J. Brian Robertson

   Article

   Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

   Inserts

   TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
   coHPH
   ACT1 terminator (S. stipitis)
   CCW12 promoter (S. stipitis)
   CaCas9
   ADH2 terminator (S. stipitis)
   ARS from S. stipitis chromosome 1
   THD3 promoter (S. stipitis)
   tRNA-sgRNA(SapI)-HDV
   ADH1 terminator (S. stipitis)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUREF-EX

   Plasmid

   #199331

   Purpose

   A pEF-BOS-EX-derived expression vector that contains an SV40 promoter-driven puromycin resistance gene

   Depositor

   Rikiro Fukunaga

   Article

   Fujii et al Genes Cells. 2023 Mar 2. doi: 10.1111/gt

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFD115

   Plasmid

   #124768

   Purpose

   pFD115 carries cas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector

   Depositor

   David Bikard

   Article

   Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)

   Inserts

   Ptet
   cas9
   term PpflB sgRNA (BsaI)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Ptet

   Available since

   Aug. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJin-264

   Plasmid

   #125717

   Purpose

   ABA-inducible split T7 gRNA for GFP vector using RNAPN d5-19

   Depositor

   Bryan Dickinson

   Article

   Pu et al ACS Chem Biol. 2018 Feb 16;13(2):431-437

   Insert

   PT7-gRNA GFP expression, PYL-linker-T7 RNAPC, T7 RNAPN (d5-19)-linker-ABI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJin-239

   Plasmid

   #125716

   Purpose

   ABA-inducible split T7 gRNA for GFP vector using RNAPN N-29-1

   Depositor

   Bryan Dickinson

   Article

   Pu et al ACS Chem Biol. 2018 Feb 16;13(2):431-437

   Insert

   pT7-gRNA GFP expression, PYL-linker-T7 RNAPC, T7 RNAPN (N-29-1)-linker-ABI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LentiCRISPRv2 RFP670

   Plasmid

   #187646

   Purpose

   lentiviral vector expressing RFP670 alongside Cas9 and an sgRNA cloning site

   Depositor

   Martin Carroll

   Insert

   RFP 670
   

   Use

   Lentiviral

   Tags

   RFP670 and sgRNA cloning site

   Expression

   Mutation

   Promoter

   EFS (P2A)

   Available since

   July 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-sgNT/Cre

   Plasmid

   #66895

   Purpose

   Expresses a non-targeting gRNA and Cre-recombinase

   Depositor

   Monte Winslow

   Article

   Chiou et al Genes Dev. 2015 Jul 15;29(14):1576-85. d

   Insert

   sgNT
   

   Use

   CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-sgNeo/Cre

   Plasmid

   #67594

   Purpose

   Expresses an Neomycin-targeting gRNA and Cre-recombinase

   Depositor

   Monte Winslow

   Article

   Chiou et al Genes Dev. 2015 Jul 15;29(14):1576-85. d

   Insert

   sgNeo
   

   Use

   CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Lenti-sgLkb1/Cre

    Plasmid

    #66894

    Purpose

    Expresses an Lkb1-targeting gRNA and Cre-recombinase

    Depositor

    Monte Winslow

    Article

    Chiou et al Genes Dev. 2015 Jul 15;29(14):1576-85. d

    Insert

    sgLkb1
    

    Use

    CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pIA123

    Plasmid

    #234038

    Purpose

    E. coli-C. difficile shuttle vector for CRISPR editing in C. difficile. Pxyl::Cas9-opt Pgdh::sgRNA-cdr_0985. PstI site to add DNA for homology directed repair. MscI and NotI for replacement of sgRNA.

    Depositor

    Craig Ellermeier

    Article

    Bollinger et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

    Inserts

    Cas9
    sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pgdh and Pxyl

    Available since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPtGE34

    Plasmid

    #107932

    Purpose

    Expresses Cas9 in Phaeodactylum tricornutum / Encodes elements required for conjugation

    Depositor

    David Edgell

    Article

    Slattery et al ACS Synth Biol. 2018 Jan 8. doi: 10.1021

    Inserts

    OriT
    40SRPS8 Promoter
    ShBle
    40SRPS8 Terminator
    Cen6-ArsH4-His3
    

    Use

    CRISPR and Synthetic Biology; Episomal vector for…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCR1265

    Plasmid

    #111094

    Purpose

    lentiviral cDNA expression vector: plenti-x1-neo-linker-IRES-Thy1.1

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCfB4586

    Plasmid

    #106124

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked Ura3 marker, integration into Yarrowia lipolytica chromosomal location IntB, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pVDM1001

    Plasmid

    #134660

    Purpose

    CRISPR delivery and repair template delivery vector

    Depositor

    Willem van Schaik

    Article

    de Maat et al FEMS Microbiol Lett. 2019 Nov 1;366(22).

    Insert

    CRISPR guide RNA array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAC-U63-gRNA2.1

    Plasmid

    #170512

    Purpose

    gRNA cloning vector containing a U6:3 promoter and a gRNA2.1 scaffold.

    Depositor

    Chun Han

    Article

    Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6:3

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGY53

    Plasmid

    #221709

    Purpose

    Empty Cas12a vector for multiplex gene editing in fungi

    Depositor

    Kyle Pomraning

    Article

    Yuan et al Front Bioeng Biotechnol. 2024 Oct 16;12:

    Type

    Empty backbone

    Use

    CRISPR; Aspergillus

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCfB6576

    Plasmid

    #106134

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_3, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCfB5791

    Plasmid

    #106126

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked Ura3 marker, integration into Yarrowia lipolytica chromosomal location IntF_2, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCfB4787

    Plasmid

    #106147

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_4, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge