162,160 results

1. pCMV-MMLVgag-3xNES-ABE8e

   Plasmid

   #181751

   Purpose

   MMLV-Gag fused to 3xNES-ABE8e with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.

   Depositor

   David Liu

   Article

   Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

   Insert

   MMLVgag-3xNES-ABE8e
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. DENV2 NS2B-NS3 protease: Co-expression of DENV-2 NS2B and NS3

   Plasmid

   #204808

   Purpose

   Bacterial co-expression of Dengue2 NS2B and NS3 proteins

   Depositor

   Frank von Delft, ASAP Discovery

   Insert

   Dengue2 NS2B (POLY Dengue2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 18, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pGEX6P1-DEST

   Plasmid

   #119749

   Purpose

   Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag.

   Depositor

   Andrew Jackson, Martin Reijns

   Type

   Empty backbone

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-Ef1a-Con/Foff 2.0-GCaMP6F

   Plasmid

   #137123

   Purpose

   Intersectional viral expression of GCaMP6F in cells expressing Cre AND NOT Flp

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Con/Foff 2.0-GCaMP6F
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   Removed RSET tag

   Promoter

   EF1a

   Available since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CAG-iGluSnFR4f-NGR-WPRE

   Plasmid

   #234452

   Purpose

   AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Insert

   iGluSnFR4f-NGR
   

   Use

   AAV

   Tags

   IgK chain and Myc epi tag-NGR GPI anchor

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSCV-FlagMLL-pl-ENL(5613)

   Plasmid

   #20873

   Depositor

   Robert Slany

   Article

   Bach et al Oncogene. 2009 Feb 12. 28(6):815-23.

   Insert

   MLL (KMT2A, MLLT1 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmyc-TNRC6B

   Plasmid

   #10978

   Depositor

   Thomas Tuschl

   Article

   Meister et al Curr Biol. 2005 Dec 6;15(23):2149-55. do

   Insert

   TNRC6B (TNRC6B Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-Epac1 SAAX

   Plasmid

   #118313

   Purpose

   Expression of human Epac1

   Depositor

   Philip Stork

   Article

   Wang et al Mol Cell Biol. 2006 Mar;26(6):2130-45. d

   Insert

   Epac1 SAAX (RAPGEF3 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. TRUPATH Triple Gagust

   Plasmid

   #196054

   Purpose

   Encodes a G alpha subunit (GNAT3) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlphaGustducin-RLuc8 (GNAT3 Human)
   GGamma1-GFP2 (GNGT1 Human)
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCI-MS2V5-PABPC1

    Plasmid

    #65807

    Purpose

    expression of PABPC1-MS2 fusion. MS2 tethers PABPC1 to mRNAs containing MS2 stem loops.

    Depositor

    Niels Gehring

    Article

    Fatscher et al RNA. 2014 Oct;20(10):1579-92. doi: 10.12

    Insert

    PAB1 (PABPC1 Human)
    

    Use

    Tags

    MS2 stem loops and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. DPYSL2-pLJC2

    Plasmid

    #189866

    Purpose

    Expresses human DPYSL2 (CRMP2) in mammalian cells

    Depositor

    Yoav Shaul

    Article

    Abu Rmaileh et al J Cell Biol. 2022 Jul 4;221(7). pii: 213

    Insert

    DPYSL2 (DPYSL2 Human)
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYTK072

    Plasmid

    #65179

    Purpose

    Encodes ConRE as a Type 5 part to be used in the Dueber YTK system

    Depositor

    John Dueber

    Article

    Lee et al ACS Synth Biol. 2015 Apr 14.

    Insert

    ConRE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR myc-MBP-SspB-iRFP-NLS

    Plasmid

    #223286

    Purpose

    An SspB-tagged maltose binding protein (MBP) with an infrared fluorescent protein for visualization. Used to demonstrate condensate dissolution using the OptoMBP system.

    Depositor

    Jared Toettcher

    Article

    Brumbaugh-Reed et al bioRxiv 2024.01.16.575860

    Insert

    Myc-MBP-SspB-irFP-NLS
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. CL20_mEGFP_PML_RARA

    Plasmid

    #205897

    Purpose

    Express mEGFP-tagged fusion protein, PML_RARA from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    PML_RARA (PML, RARA Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pXPR_501

    Plasmid

    #96922

    Purpose

    for CRISPRa, lentiviral expression of gRNA scaffold using activator VP64

    Depositor

    David Root, John Doench

    Article

    Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV phSyn1(S)-FLEX-tdTomato-WPRE

    Plasmid

    #51505

    Purpose

    Can be used to generate AAV virus that will express tdTomato in the presence of Cre in neurons from the synapsin promoter

    Depositor

    Hongkui Zeng

    Article

    Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

    Insert

    tdTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    phSyn1

    Available since

    May 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUCP20T-dsRedExpress

    Plasmid

    #78470

    Purpose

    Broad range bacterial fluorescence labeling

    Depositor

    Mariette Barbier

    Article

    Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

    Insert

    dsRedExpress
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA3-myc3-ROC1

    Plasmid

    #20717

    Depositor

    Yue Xiong

    Article

    He et al Genes Dev. 2006 Nov 1. 20(21):2949-54.

    Insert

    RING finger protein 1 (ROC1) (RBX1 Human)
    

    Use

    Tags

    3xMyc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. luxA+luxB/pRSETB Duet

    Plasmid

    #197581

    Purpose

    Expressing luxA and luxB under dual T7 promoter

    Depositor

    Takeharu Nagai

    Article

    Kaku et al Sci Rep. 2021 Jul 22;11(1):14994. doi: 1

    Insert

    LuxA LuxB
    

    Use

    Tags

    T7 tag and 6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pShHELIX_sgRNA_entry (CJT32)

    Plasmid

    #181782

    Purpose

    Expresses ShHELIX containing a nicking I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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