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pRSF_his6_sumo_SARS_CoV2_NSP14_P335H

Plasmid

#213525

Purpose

Expresses NSP14 of SARS-CoV-2 with a single point mutation P335H

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)

Tags

His6-sumo

Expression

Bacterial

Mutation

changed P335 to H

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_SARS_CoV2_NSP14_H424R

Plasmid

#213526

Purpose

Expresses NSP14 of SARS-CoV-2 with a single point mutation H424R

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)

Tags

His6-sumo

Expression

Bacterial

Mutation

changed H424 to R

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pUC18_BCR_GUSB_ABL1_e14a3

Plasmid

#190885

Purpose

molecular monitoring of atypical BCR::ABL1 fusion transcripts

Depositor

Nicholas Cross

Article

Schafer et al J Cancer Res Clin Oncol. 2021 Oct;147(10

Inserts

e14a3 BCR::ABL1 fusion transcript
ABL1 cDNA (ABL1 Human)
GUSB cDNA (GUSB Human)
BCR (BCR Human)

Use

Cloned cdna transcripts for use in rt-qpcr bcr::a…

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4-eIF3B MBE3 mutant

Plasmid

#133310

Purpose

Expression of luciferase driven by eIF3B promoter region mutated at the E-box binding motif 3

Depositor

Josep Domingo-Domenech

Article

Santasusagna et al Cancer Discov. 2023 Sep 7. doi: 10.1158/

Insert

eIF3B promoter MBE3 mutant (EIF3B Human)

Use

Luciferase

Expression

Mammalian

Mutation

ccacgtgacc changed to cAaAAAAacc

Promoter

eIF3B

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pOCC102-MBP-eIF4G-Sk

Plasmid

#219976

Purpose

eIF4G-Sk protein purification

Depositor

Simon Alberti

Insert

eIF4G-Sk

Tags

6xHis-MBP

Expression

Insect

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLifeact-mScarlet3-H (mYongHong)

Plasmid

#239415

Purpose

Expresses Lifeact-mScarlet3-H (aka mYongHong) in mammalian cells

Depositor

Zhifei Fu

Article

Xiong et al Nat Methods. 2025 Apr 17. doi: 10.1038/s

Insert

Lifeact-mScarlet3-H

Expression

Mammalian

Mutation

M163H in mScarlet3

Promoter

CMV

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pKDEL-mScarlet3-H (aka mYongHong)

Plasmid

#239411

Purpose

Expresses mScarlet3-H (aka mYongHong) localized to ER in mammalian cells

Depositor

Zhifei Fu

Article

Xiong et al Nat Methods. 2025 Apr 17. doi: 10.1038/s

Insert

mScarlet3-H-KDEL

Expression

Mammalian

Mutation

M163H in mScarlet3

Promoter

CMV

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-EGFP-ALDH1A1-Neo

Plasmid

#189742

Purpose

The construct was used to express GFP tagged human ALDH1A1 in mammalian cells for the analysis of the subcellular location of this protein.

Depositor

Robert Sobol

Article

Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

Insert

ALDH1A1 (ALDH1A1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

PGK

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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peGFP-N1-Cofilin

Plasmid

#186747

Purpose

Expresses wildtype GFP-tagged human cofilin in mammalian cells

Depositor

Geert van den Bogaart

Article

Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

Insert

Cofilin-1 (CFL1 Human)

Tags

GFP

Expression

Mammalian

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pmCherry-N1-cofilin

Plasmid

#186750

Purpose

Expresses wildtype mCherry-tagged human cofilin in mammalian cells

Depositor

Geert van den Bogaart

Article

Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

Insert

Cofilin-1 (CFL1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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peGFP-N1-Cofilin-S41E

Plasmid

#186748

Purpose

Expresses S41E phosphomimetic GFP-tagged human cofilin in mammalian cells

Depositor

Geert van den Bogaart

Article

Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

Insert

Cofilin-1 (CFL1 Human)

Tags

GFP

Expression

Mammalian

Mutation

Changed serine 41 to glutamic acid

Promoter

CMV

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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peGFP-N1-Cofilin-S41A

Plasmid

#186749

Purpose

Expresses S41A phosphodead GFP-tagged human cofilin in mammalian cells

Depositor

Geert van den Bogaart

Article

Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

Insert

Cofilin-1 (CFL1 Human)

Tags

GFP

Expression

Mammalian

Mutation

Changed serine 41 to alanine

Promoter

CMV

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pmCherry-N1-Cofilin-S41E

Plasmid

#186751

Purpose

Expresses S41E phosphomimetic mCherry-tagged human cofilin in mammalian cells

Depositor

Geert van den Bogaart

Article

Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

Insert

Cofilin-1 (CFL1 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Changed serine 41 to glutamic acid

Promoter

CMV

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS/XBB.1.5-RBD

Plasmid

#213038

Purpose

Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.XBB.1.5 variant

Depositor

Priyamvada Acharya

Article

Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

Insert

pCAGGS/XBB.1.5-RBD (S )

Tags

6 His Tag

Expression

Mammalian

Mutation

G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Omicron.BA.5-RBD

Plasmid

#213478

Purpose

Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.BA.5 variant

Depositor

Priyamvada Acharya

Article

Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

Insert

pCAGGS/Omicron.BA.5 RBD (S )

Tags

6 His Tag

Expression

Mammalian

Mutation

G339D,R346T,S371F,S373P,S375F,T376A,D405N,R408S,K…

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS/EG5-RBD

Plasmid

#213410

Purpose

Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.EG5 variant

Depositor

Priyamvada Acharya

Article

Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

Insert

pCAGGS/EG5-RBD (S )

Tags

6 His Tag

Expression

Mammalian

Mutation

G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR223-PARK7H126A

Plasmid

#115180

Purpose

Gateway cloning of PARK7H126A into any destination vector

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

PARK7H126A (PARK7 Human)

Use

Gateway cloning into appropriate destination vect…

Mutation

p.H126A

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR223-PARK7E163K

Plasmid

#115181

Purpose

Gateway cloning of PARK7E163K into any destination vector

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

PARK7E163K (PARK7 Human)

Use

Gateway cloning into appropriate destination vect…

Mutation

p.E163K

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR223-PARK7V51G

Plasmid

#115178

Purpose

Gateway cloning of PARK7V51G into any destination vector

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

PARK7V51G (PARK7 Human)

Use

Gateway cloning into appropriate destination vect…

Mutation

p.V51G

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR223-PARK7C53A

Plasmid

#115179

Purpose

Gateway cloning of PARK7C53G into any destination vector

Depositor

Mohan Babu

Article

Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

Insert

PARK7C53A (PARK7 Human)

Use

Gateway cloning into appropriate destination vect…

Mutation

p.C53A

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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