We narrowed to 30,867 results for: PLE;

Showing: 11261 - 11280 of 30867 results

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pcDNA3.1(+)/UUG-nLuc-3XFLAG

Plasmid

#127302

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from UUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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tdKatushka2-VASP-5

Plasmid

#56049

Purpose

Localization: Actin/Focal Adhesions, Excitation: 586, Emission: 632

Depositor

Michael Davidson

Article

Shcherbo et al Biochem J. 2009 Mar 15;418(3):567-74. do

Insert

VASP

Tags

tdKatushka2

Expression

Mammalian

Mutation

A209T in VASP

Promoter

CMV

Available Since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pSB700 lenti gRNA Cerulean-zhang2.0 Weiss full

Plasmid

#79383

Purpose

Cas9 gRNA variant to recruit ms2 proteins

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

Zhang 2.0 + scaffold

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDGE476

Plasmid

#153267

Purpose

M2E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pAGM35171

Plasmid

#153222

Purpose

Level 1 position 1 part containing the Nos promoter, the BAR gene, and the Ocs terminator

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Insert

Nos promoter, BAR gene, Ocs terminator

Use

Synthetic Biology

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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A3Bi-Cas9n-UGI

Plasmid

#119135

Purpose

Base editor made from full-length APOBEC3B with an L1 intron to prevent self-restriction

Depositor

Reuben Harris

Article

Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

Insert

APOBEC3B L1 intron-Cas9 nickase-UGI

Use

CRISPR

Mutation

L1 intron insertion

Promoter

CMV

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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p6899 PHAGE-P CMVT N-HA-hE6AP II C843A

Plasmid

#37604

Depositor

Peter Howley

Article

Martinez-Noel et al Mol Cell Biol. 2012 May 29.

Insert

E6AP II C843A (UBE3A Human)

Tags

HA

Expression

Mammalian

Mutation

C843A

Promoter

CMVt

Available Since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only
pH2B-miRFP718nano

Plasmid

#195750

Purpose

Histone 2B labelled with near-infrared fluorescent protein miRFP718nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

Insert

H2B-miRFP718nano

Tags

miRFP718nano

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pAGM65905

Plasmid

#153217

Purpose

Level 2 cloning vector for one or several guide RNAs, already contains Cas9 and a kanamycin selection cassette for plant transformation

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pSUPERIORpuro-shGAS5

Plasmid

#46370

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Renganathan et al Mol Cancer. 2014 May 23;13:119. doi: 10.

Insert

GAS5 (GAS5 Human)

Use

RNAi

Promoter

H1

Available Since

Aug. 16, 2013

Availability

Academic Institutions and Nonprofits only
pFA-DAB2

Plasmid

#162690

Purpose

Expresses DabAB2 inorganic carbon transport complex

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

DabAB2 inorganic carbon transport complex

Expression

Bacterial

Promoter

PLtet0-1 promoter

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-YdLight1

Plasmid

#125705

Purpose

Expresses the yellow genetically encoded dopamine sensor YdLight1 in mammalian cells.

Depositor

Lin Tian

Article

Patriarchi et al Nat Methods. 2020 Nov;17(11):1147-1155.

Insert

YdLight1

Tags

Flag tag

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pDGE667

Plasmid

#153232

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Tags

GFP

Expression

Plant

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRS315-ADH700p-yeCherry-p150-yeGFP-CYCt

Plasmid

#194519

Purpose

Dual fluorescence reporter for studying protein degradation

Depositor

Tommer Ravid

Article

Mashahreh et al Nat Commun. 2022 Dec 8;13(1):7588. doi:

Insert

TIF4631 leader sequence

Expression

Yeast

Available Since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

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Flag Nprl2 pLJM1

Plasmid

#46338

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Nprl2 (NPRL2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only
pscALPS-SIV-CPZ-TAN3 vpr

Plasmid

#115833

Purpose

Encodes codon optimized 3xFLAG-SIVCPZ-TAN3 Vpr (DQ374658) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVCPZ-TAN3 Vpr (DQ374658)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pBABE-puro-mIR-31 sponge

Plasmid

#22694

Purpose

Stable suppression of miR-31 function

Depositor

Bob Weinberg

Article

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Insert

miR-31 (MIR31 Human)

Use

Retroviral

Tags

*

Expression

Mammalian

Mutation

miR-31 sponge with 7x tandem binding sites to the…

Available Since

March 11, 2010

Availability

Academic Institutions and Nonprofits only
pMDJ21 - pEXP [Psmu-1 | eFlp recombinase (PATCs) | smu-1 UTR]

Plasmid

#159810

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP [Psmu-1 | eFlp recombinase (PATCs) | smu-1 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV/3' Box_(GLuc)_INT

Plasmid

#68436

Purpose

Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

INT construct_bearing three PP7 Stem-loops

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2015

Availability

Academic Institutions and Nonprofits only

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PB-CT3G-ERP2-MG-IRES2-mNeonGreen

Plasmid

#175506

Purpose

All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (hEF1a-driven rtTA and puromycin resistance)

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3G-mCMV

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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