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pAAV-GFAP-HA-hM4D(Gi)-IRES-mCitrine

Plasmid

#50471

Purpose

Gi-coupled hM4D-IRES-mCitrine under the control of GFAP promoter

Depositor

Bryan Roth

Insert

hM4D(Gi)-IRES-mCitrine (CHRM4 Human)

Use

AAV

Tags

HA

Mutation

See supplemental documents for DREADD mutations

Promoter

GFAP

Available since

April 16, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pTXB1-sfGFP

Plasmid

#51562

Purpose

Expression of superfolder GFP for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone)

Depositor

Sankar Adhya, Francisco Malagon

Insert

superfolder GFP

Tags

Intein-Chiting Binding Domanin

Expression

Bacterial

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pSIR-DsRed-Express2

Plasmid

#51135

Purpose

Self-inactivating retrovirus vector containing the DsRed-Express2 gene

Depositor

Hodaka Fujii

Publication

Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available since

Feb. 19, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pmRFP-N1 human cofilin S3A

Plasmid

#50857

Purpose

expresses constituvely active cofilin fused to mRFP in mammalian cells

Depositor

James Bamburg

Publication

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 S3A (CFL1 Human)

Tags

mRFP

Expression

Mammalian

Mutation

S3A constitutively active

Promoter

CMV

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
Twitch-3 pcDNA3

Plasmid

#49532

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Publication

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch-3

Tags

ECFP and cpCit174

Expression

Mammalian

Promoter

CMV

Available since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3 HA-SUMO2-Q90P

Plasmid

#36448

Purpose

Expresses HA-Sumo2Q90P mutant in mammalian cells

Depositor

Guy Salvesen

Publication

Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

Insert

SUMO2 (SUMO2 Human)

Tags

HA

Expression

Mammalian

Mutation

Mutated Q90P

Promoter

T7

Available since

Oct. 23, 2013

Availability

Academic Institutions and Nonprofits only
pSNR52-sgTEF1

Plasmid

#46922

Purpose

Yeast CEN/ARS vector (Ura3) that contains sgRNA controlled by SNR 52 promoter, targeting endogenous TEF1 promoter

Depositor

Stanley Qi, Jonathan Weissman

Publication

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Insert

sgTEF1 promoter (TEF1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pThy1-Autobow

Plasmid

#45187

Depositor

Joshua Sanes

Publication

Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

Insert

pThy1-Autobow

Use

Linearized for transgenic mouse injection

Promoter

Thy1

Available since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pECIA14

Plasmid

#47051

Purpose

This is a Gateway destination vector for secreted expression in Drosophila cell culture by CuSO4 induction. Inserts will be pentamerized and tagged with Alkaline phosphatase, FLAG and His tags

Depositor

Chris Garcia

Publication

Ozkan et al Cell. 2013 Jul 3;154(1):228-39. doi: 10.

Type

Empty backbone

Use

Secreted expression in drosophila culture

Tags

Alkaline Phosphatase (human, placental), Flag, HR…

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available since

Aug. 30, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pNkx-GFP

Plasmid

#45497

Depositor

Sean Wu

Publication

Wu et al Cell. 2006 Dec 15;127(6):1137-50. Epub 2

Insert

2.5kb mouse Nkx2.5 enhancer and promoter fragment (Nkx2-5 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

2.5kb Nkx2.5 enhancer and promoter fragment

Available since

Aug. 22, 2013

Availability

Academic Institutions and Nonprofits only

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SAFB1-EGFP

Plasmid

#32746

Depositor

Steffi Oesterreich

Publication

Townson et al Clin Cancer Res. 2000 Sep;6(9):3788-96.

Insert

SAFB1 (SAFB Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only
pGEX4T-1_WT_OCT4

Plasmid

#40633

Depositor

James Thomson

Publication

Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

POU5F1 (POU5F1 Human)

Tags

GST, TEV cleavage, and Thrombin cleavage

Expression

Bacterial

Promoter

tac

Available since

July 26, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCM62

Plasmid

#45826

Depositor

Mary Lidstrom

Publication

Marx et al Microbiology. 2001 Aug;147(Pt 8):2065-75

Type

Empty backbone

Use

Broad host range cloning vector

Promoter

lac

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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mH2A1.2-CT-GFP

Plasmid

#45169

Depositor

Brian Chadwick, Hunt Willard

Publication

Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

Insert

H2Afy (MACROH2A1 Human)

Tags

GFP

Expression

Mammalian

Available since

July 2, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFA6a-TRP1

Plasmid

#41595

Depositor

John Pringle

Publication

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

TRP1 (TRP1 Budding Yeast)

Use

Yeast genomic targeting

Available since

May 15, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Active Wnt4-V5

Plasmid

#43812

Depositor

Xi He

Publication

MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

Insert

WNT4 (WNT4 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3-MTS-CA-c-Src-FLAG

Plasmid

#44654

Depositor

Yoshimi Homma

Publication

Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

Insert

v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)

Tags

FLAG tag and mitochondria-targeting sequence (MTS…

Expression

Mammalian

Mutation

changed Tyr 530 to Phe

Promoter

CMV promoter

Available since

May 6, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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PREX2 pcdna3.1 V5/his

Plasmid

#41555

Depositor

Ramon Parsons

Publication

Fine et al Science. 2009 Sep 4;325(5945):1261-5.

Insert

PREX2a (PREX2 Human)

Tags

V5 and his

Expression

Mammalian

Promoter

T7

Available since

Feb. 5, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
IL2 pMalLP

Plasmid

#41808

Depositor

Chris Garcia

Publication

Levin et al Nature. 2012 Mar 25;484(7395):529-33. do

Insert

IL2 (IL2 Human)

Tags

3C protease site, 6x His, and Maltose Binding Pro…

Expression

Bacterial

Promoter

pTac

Available since

Jan. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pHMGWA-Pa14Csy4

Plasmid

#41091

Depositor

Jennifer Doudna

Publication

Haurwitz et al Science. 2010 Sep 10;329(5997):1355-8.

Insert

Csy4

Tags

His6-MBP

Expression

Bacterial

Mutation

Wild type

Promoter

T7

Available since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only