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We narrowed to 11,840 results for: CHL

Showing: 11341 - 11360 of 11840 results
  1. pEVOL-ABK

    Plasmid
    #126035
    Purpose
    Efficient expression of tRNA synthetase/tRNA for the incorporation of a photo-cross-linker, 3’-azibutyl-N-carbamoyl-lysine (AbK) into recombinant protein by amber codon (TAG) suppression in E. coli.
    Depositor
    Inserts
    tRNA synthetase 1
    tRNA synthetase 2
    tRNA for TAG codon
    Expression
    Bacterial
    Mutation
    L274M, C313A, Y349F
    Promoter
    araBAD promoter, glnS promoter, and proK promoter
    Available Since
    Sept. 10, 2019
    Availability
    Academic Institutions and Nonprofits only
  2. pCAT-YFP-TetR

    Plasmid
    #59018
    Purpose
    Expresses a fusion of yellow fluorescent protein (YFP) to the N-terminus of the Escherichia coli Tn10 tet repressor (TetR) driven by the alpha tubulin promoter for use in T. gondii
    Depositor
    Insert
    YFP-TetR
    Use
    Toxoplasma expression
    Promoter
    alpha-tubulin
    Available Since
    Sept. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
  3. pJL046

    Plasmid
    #198807
    Purpose
    In vivo neuronal inhibition through histamine-gated chloride channel. Neurons expressing HisCl1 transgene are inhibited after addition of histamine.
    Depositor
    Insert
    15xUAS::HisCl1-SL2-GFP::let-858 3'UTR
    Expression
    Worm
    Available Since
    May 2, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. pCK340

    Plasmid
    #192639
    Purpose
    Expresses dSpG and MCP-SoxS(R93A/S101A) on p15A-CmR
    Depositor
    Inserts
    Sp.pCas9-dSpG
    BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR and Synthetic Biology
    Mutation
    SoxS has R93A and S101A mutations and dSpG has se…
    Promoter
    BBa_J23107 and Sp.pCas9
    Available Since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
  5. pGLTR-X-GFP

    Plasmid
    #58245
    Purpose
    GATEWAY-compatible lentiviral conditional RNAi delivery vector; SFFV-driven expression of TetR and eGFP
    Depositor
    Inserts
    attR1-Cm(R)-CcdB-attR2
    TetR-NLS-T2A-GFP
    Use
    Lentiviral and RNAi; Gateway destionation vector
    Tags
    GFP, NLS, T2A, and TetR
    Expression
    Mammalian
    Promoter
    SFFV and no
    Available Since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
  6. pBpaRS3tRNA

    Plasmid
    #197102
    Purpose
    To express the p-benzoylphenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into p-benzoylphenylalanine.
    Depositor
    Insert
    TyrRS variant, amber suppressor tRNA
    Expression
    Bacterial
    Mutation
    Gly32, Ser107, Thr158, Ser159, Arg286 (TyrRS)
    Available Since
    June 15, 2023
    Availability
    Academic Institutions and Nonprofits only
  7. pEM-Cas9HF1

    Plasmid
    #89961
    Purpose
    Modified from pCas9-CR4 (Addgene: 62655) to use the high-fidelity version of Cas9, SpCas9-HF1 (N497A/R661A/Q695A/Q926A) from Kleinstiver et al 2016.
    Depositor
    Insert
    Cas9HF1
    Expression
    Bacterial
    Mutation
    N497A/R661A/Q695A/Q926A
    Promoter
    pTet
    Available Since
    June 19, 2017
    Availability
    Academic Institutions and Nonprofits only
  8. pTSara

    Plasmid
    #60720
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by the arabinose inducible promoter PBAD. Also contains a constitutive araC ORF
    Depositor
    Inserts
    N Terminal fragment of T7 RNAP split betwen 179-180
    N Terminal fragment of T7 RNAP split betwen 179-180
    Expression
    Bacterial
    Mutation
    T7 RNAP split between positions 179-180 and wt T7…
    Promoter
    PBAD
    Available Since
    Dec. 18, 2014
    Availability
    Academic Institutions and Nonprofits only
  9. pHW449

    Plasmid
    #198805
    Purpose
    Direct light-gated cation channel from Chlamydomonas reinhardtii that allows neuron deploarization through brief pulses of blue light.
    Depositor
    Insert
    15xUAS::hChR2(H134R)-YFP::let-858 3'UTR
    Expression
    Worm
    Available Since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 11341 - 11360 of 11840 results