We narrowed to 12,475 results for: pcdna

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pcDNA3-ExRai-AktAR2

Plasmid

#184047

Purpose

Improved excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity in living cells.

Depositor

Jin Zhang

Article

Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

Insert

ExRai-AktAR2

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-N-Flag-NLRP3

Plasmid

#75127

Purpose

Expresses N-terminal Flag-tagged NLRP3 in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

NLR family, pyrin domain containing 3 (Nlrp3 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.4_7C11-1 heavy chain

Plasmid

#198251

Purpose

recombinant expression of heavy chain of monoclonal anti-AMP antibody 7C11-1

Depositor

Aymelt Itzen

Article

Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

Insert

heavy chain of monoclonal anti-AMP antibody 7C11-1 (mouse IgG2a)

Tags

IL10 signal peptide

Expression

Mammalian

Promoter

CMV

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Peredox-mCherry

Plasmid

#32383

Depositor

Gary Yellen

Article

Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

Insert

Peredox-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA GFP10-ZIP

Plasmid

#182241

Purpose

Tripartite split-GFP mammalian. Express leucine zipper with GFP10

Depositor

Stéphanie Cabantous

Article

Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

Insert

GFP10-ZIP

Tags

split-GFP10 tripartite

Expression

Mammalian

Promoter

CMV

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA ZIP-GFP11

Plasmid

#182242

Purpose

Tripartite split-GFP mammalian. Express leucine zipper with GFP11

Depositor

Stéphanie Cabantous

Article

Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

Insert

ZIP-GFP11

Tags

split-GFP11 tripartite

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp5

Plasmid

#157685

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp5 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp5 (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-FLAG-LKB1

Plasmid

#8590

Depositor

Lewis Cantley

Article

Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

Insert

LKB1 (STK11 Human)

Tags

FLAG

Expression

Mammalian

Available Since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-N-Myc_WT USP7

Plasmid

#131242

Purpose

Mammalian expression of N-terminally Myc-tagged USP7

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV enhancer + CMV promoter

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3/mStayGold(c4)=UtrCH

Plasmid

#212020

Purpose

For filamentous actin labeling. Alternatively, the UtrCH gene can be replaced with a target gene for N-terminal tagging with mStayGold via a c4 adaptor and a linker [(GGGGS)3] (denoted as ‘=’).

Depositor

Atsushi Miyawaki

Article

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

UtrCH

Tags

mStayGold(c4)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-GFP-IMP2-2

Plasmid

#42175

Depositor

Alexandra Kiemer

Article

Kessler et al Am J Physiol Gastrointest Liver Physiol.

Insert

Insulin-like growth factor 2 mRNA binding protein 2-2 (IGF2BP2 Human)

Tags

GFP

Expression

Mammalian

Promoter

T7

Available Since

June 14, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-PDGFRA-V5/HIS

Plasmid

#201987

Purpose

expression of human PDGFRA receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

PDGFRA receptor tyrosine kinase (PDGFRA Human)

Tags

V5, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_H2B_HaloTag7

Plasmid

#169329

Purpose

Expression of H2B-HaloTag7 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

H2B-HaloTag7 (H2BC21 Human)

Tags

HaloTag7

Expression

Mammalian

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_HaloTag7_SKL

Plasmid

#175532

Purpose

Peroxysomal expression of HaloTag7 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

HaloTag7-SKL

Expression

Mammalian

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag FKHR

Plasmid

#13507

Depositor

Kunliang Guan

Article

Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-

Insert

FKHR (FOXO1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Dec. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Floxed-STOP mCherry

Plasmid

#122963

Purpose

mCherry-based fluorescent reporter for Cre-loxP DNA recombination reaction

Depositor

Moritoshi Sato

Article

Kawano et al Nat Chem Biol. 2016 Dec;12(12):1059-1064

Insert

pcDNA3.1_Floxed-STOP mCherry

Use

Cre/Lox

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3-SARS2-B.1.617.2

Plasmid

#172320

Purpose

expressing SARS-CoV-2 B.1.617.2 spike protein (delta strain) for pseudovirus production.

Depositor

David Nemazee

Article

Cho et al Sci Transl Med. 2021 Oct 20;13(616):eabj

Insert

Spike of B.1.617.2 strain (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19R, 156G, 157-158del, L452R, T478K, D614G, P681…

Promoter

CMV-F

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3/Flag-WTAP

Plasmid

#53741

Purpose

For expression of WTAP (Wilms tumor 1 associated protein) in human cells

Depositor

Chuan He

Article

Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

Insert

Wilms tumor 1 associated protein (WTAP Human)

Tags

FLAG tag

Expression

Mammalian

Promoter

T7

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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RGS-6xHis-pcDNA3.1-

Plasmid

#52534

Purpose

enables eukaryotic expression of N-terminally RGS-6xHis–tagged fusion proteins

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Type

Empty backbone

Tags

RGS-6xHis

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ExRai-AMPKAR(T/A)

Plasmid

#192447

Purpose

Non-phosphorylatable negative-control mutant of ExRai-AMPKAR.

Depositor

Jin Zhang

Article

Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

Insert

ExRai-AMPKAR(T/A)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

Expression

Mammalian

Mutation

Thr 6 in AMPK substrate mutated to Ala.

Promoter

CMV

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-HA-eLACCO2.1-NGR

Plasmid

#208023

Purpose

Biosensor for extracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

eLACCO2.1

Expression

Mammalian

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/cyto-iAspSnFR

Plasmid

#201394

Purpose

CMV overexpression of cyto-iAspSnFR in mammalian cell lines

Depositor

Kai Johnsson

Article

Hellweg et al Cell Chem Biol. 2024 May 20:S2451-9456(2

Insert

cyto-iAspSnFR

Expression

Mammalian

Mutation

gltI5-253: Y42F, S72A, T92D, G111T, K192R, Y211W,…

Promoter

CMV

Available Since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-Smad4

Plasmid

#80888

Purpose

mammalian expression of Smad4

Depositor

Aristidis Moustakas

Article

Moren et al Oncogene. 2000 Sep 7;19(38):4396-404.

Insert

Smad4 (SMAD4 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc3-CUL4A

Plasmid

#19951

Depositor

Yue Xiong

Article

Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

Insert

CUL4A (cullin 4A) (CUL4A Human)

Tags

myc3

Expression

Mammalian

Available Since

April 1, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-ExRai-AKAR2

Plasmid

#161753

Purpose

Enhanced excitation-ratiometric biosensor for monitoring Protein Kinase A activity in living cells and in vivo.

Depositor

Jin Zhang

Article

Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

Insert

ExRai-AKAR2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-KIT-V5/HIS

Plasmid

#201986

Purpose

expression of human KIT receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

KIT receptor tyrosine kinase (KIT Human)

Tags

V5, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Claspin-Flag

Plasmid

#136246

Purpose

Protein expression of Claspin with a C terminal FLAG tag

Depositor

Aziz Sancar

Article

Sar et al J Biol Chem. 2004 Sep 17;279(38):39289-9

Insert

Claspin

Tags

Flag

Expression

Mammalian

Mutation

E263G, Q499R, I783T, D932G and S1062G- Please see…

Available Since

Jan. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-endo-Nb37

Plasmid

#205514

Purpose

Endosome-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.

Depositor

Jin Zhang

Article

Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

Insert

endo-Nb37

Tags

2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA4-GFP-Vinculin

Plasmid

#107153

Purpose

mammalian expression

Depositor

Tina Izard

Article

Chinthalapudi et al J Cell Biol. 2014 Dec 8;207(5):643-56. d

Insert

Vinculin (VCL Human)

Tags

(His)10-GFP

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRTTO-NHASt-codoptUL33

Plasmid

#135103

Purpose

Codon-optimized HSV-1 UL33 with N-terminal HAStrep tag

Depositor

Markus Landthaler

Article

Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

Insert

HSV-1 UL33 codon optimized

Tags

HA-Strep-Strep

Expression

Mammalian

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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