We narrowed to 18,831 results for: Tec

1. pLH-sgRNA1-2XPP7

   Plasmid

   #75390

   Purpose

   sgRNA1-2XPP7

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

   Insert

   sgRNA1-2XPP7
   

   Use

   CRISPR and Lentiviral

   Tags

   no

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pColE1_sgRNA_pos6_AmpR

   Plasmid

   #119149

   Purpose

   Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR

   Depositor

   Vincent Noireaux

   Article

   Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

   Insert

   sgRNA targeting position 6 in pColE1_70a_deGFP_KanR
   

   Use

   Crispr

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPICZA_CeTRIC-B1_Del

   Plasmid

   #87344

   Purpose

   Inducible expression of CeTRIC-B1 in P.Pichia strains

   Depositor

   Zhenfeng Liu

   Article

   Yang et al Nature. 2016 Oct 27;538(7626):537-541. d

   Insert

   TRIC-B1
   

   Use

   Tags

   polyhistidine

   Expression

   Yeast

   Mutation

   Δ248-297

   Promoter

   AOX1

   Available Since

   Feb. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBad33_ISAam1_reRNA

   Plasmid

   #205322

   Purpose

   Bacterial expression of ISAam1 TnpB and its reRNA

   Depositor

   Haoyi Wang

   Article

   Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

   Insert

   ISAam1 TnpB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBb(RK2)1k-GFPuv

   Plasmid

   #106394

   Purpose

   IPTG-inducible trc promoter expressing gfpuv on RK2 origin

   Depositor

   Brian Pfleger

   Article

   Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

   Insert

   GFPuv
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   trc

   Available Since

   March 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSCVII

   Plasmid

   #162750

   Purpose

   MSCV backbone

   Depositor

   Maki Nakayama

   Article

   Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pU6-As-crRNA

   Plasmid

   #78956

   Purpose

   Cloning vector for expression of AsCpf1 crRNA. It contains BsmB1 site for cloning.

   Depositor

   Jin-Soo Kim

   Article

   Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available Since

   June 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMT406

   Plasmid

   #139384

   Purpose

   RPU standard, pNBU1 backbone with BsaI sites, AmpR, ErmR

   Depositor

   Christopher Voigt

   Article

   Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

   Insert

   RPU standard
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   No promoter

   Available Since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSET-dCas9

   Plasmid

   #110183

   Purpose

   Expression of the dCas9 gene in Streptomyces

   Depositor

   Yinhua Lu

   Article

   Zhao et al Biotechnol J. 2018 Jun 3. doi: 10.1002/b

   Insert

   dCas9
   

   Use

   CRISPR; I

   Tags

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   ermE*p

   Available Since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDA303

    Plasmid

    #195199

    Purpose

    Overexpressing 6His-TetR in E. coli.

    Depositor

    Rahul Sarpeshkar

    Article

    Deng et al Front Bioeng Biotechnol. 2022 Sep 28;10:

    Insert

    TetR
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T5 promoter

    Available Since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMKV058

    Plasmid

    #133313

    Purpose

    BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+ and WUS2

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    Luc+. WUS2
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtUbi10, Nos

    Available Since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSCVII-tdTomato

    Plasmid

    #162752

    Purpose

    Retroviral (MSCV) expression of td Tomato fluorescent protein

    Depositor

    Maki Nakayama

    Article

    Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

    Insert

    tdTomato
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-UAS-CITRINE

    Plasmid

    #104839

    Purpose

    SPARK reporter gene

    Depositor

    Alice Ting

    Article

    Kim et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

    Insert

    Citrine
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UAS

    Available Since

    Jan. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFastBac cloning vector with BioBrick PolyPromoter LIC Subcloning (438-A)

    Plasmid

    #55218

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    June 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBBRBB-Ppuf843-1200-DsRed

    Plasmid

    #50962

    Purpose

    Expressed DsRed using the Ppuf843-1200 promoter segment, for use in R. sphaeroides

    Depositor

    Claudia Schmidt-dannert

    Article

    Tikh et al Appl Microbiol Biotechnol. 2014 Feb 9.

    Insert

    DsRed
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Ppuf843-1200

    Available Since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pU6-Lb-crRNA

    Plasmid

    #78957

    Purpose

    Cloning vector for expression of LbCpf1 crRNA. It contains BsmB1 site for cloning.

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available Since

    June 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHAGE-EFS-N22p-3XRFPnls

    Plasmid

    #75387

    Purpose

    N22p-3XRFPnls

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    N22p
    

    Use

    Lentiviral

    Tags

    3XmCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MCP-M-MLV-RT

    Plasmid

    #181799

    Purpose

    MCP-M-MLV-RT (MS2-N55K-MMLVRT)

    Depositor

    Erik Sontheimer

    Article

    Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

    Insert

    MCP-M-MLV-RT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUC57-Curlcake3

    Plasmid

    #139342

    Purpose

    Expresses Curlcake3 (CC3) in E.coli

    Depositor

    Eva Maria Novoa

    Article

    Liu et al Nat Commun. 2019 Sep 9;10(1):4079. doi:

    Insert

    Curlcake3 with T7 promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pColE1_sgRNA_NT_AmpR

    Plasmid

    #119144

    Purpose

    Encodes non-targeting sgRNA

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    non-targeting sgRNA
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA-miR-21 toehold switch

    Plasmid

    #184756

    Purpose

    Expresses miR-21 sensor with RFP output

    Depositor

    Allen Liu

    Article

    Wang et al ACS Synth Biol. 2019 May 17;8(5):1079-10

    Insert

    Red Fluorescence Protein
    

    Use

    Tags

    miR-21 toehold switch

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pUC57-Curlcake1

    Plasmid

    #139340

    Purpose

    Expresses Curlcake1 (CC1) in E.coli

    Depositor

    Eva Maria Novoa

    Article

    Liu et al Nat Commun. 2019 Sep 9;10(1):4079. doi:

    Insert

    Curlcake1 with T7 promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pUC57-Curlcake2

    Plasmid

    #139341

    Purpose

    Expresses Curlcake2 (CC2) in E.coli

    Depositor

    Eva Maria Novoa

    Article

    Liu et al Nat Commun. 2019 Sep 9;10(1):4079. doi:

    Insert

    Curlcake2 with T7 promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pUC57-Curlcake4

    Plasmid

    #139343

    Purpose

    Expresses Curlcake4 (CC4) in E.coli

    Depositor

    Eva Maria Novoa

    Article

    Liu et al Nat Commun. 2019 Sep 9;10(1):4079. doi:

    Insert

    Curlcake4 with T7 promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGEL850

    Plasmid

    #214250

    Purpose

    Expression TadCBEa in rice cells

    Depositor

    Yong Zhang

    Article

    Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pICSL11015

    Plasmid

    #117499

    Purpose

    FAST-Red selectable marker Golden Gate Level 1 Position 1

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BpiI:TGCC:pOLE1:OLE1-RFP:OLE1t:GCAA:BpiI
    

    Use

    Tags

    RFP

    Expression

    Plant

    Mutation

    Promoter

    AtOLE1

    Available Since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. p104_gRNA_Sp_CD3e

    Plasmid

    #213780

    Purpose

    Expresses CRISPRi gRNA for CD3e promoter and GFP. gRNA driven by mU6.

    Depositor

    Howard Chang

    Article

    Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    Sp gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. ABE-RA2.1

    Plasmid

    #208300

    Purpose

    Expresses ABE-RA2.1 in mammalian cells

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA-RA2.1-SpCas9 D10A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    P48A, R51H, D108G, K110R, H122R, M126I, N127K

    Promoter

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMSCVII-BFP

    Plasmid

    #162755

    Purpose

    Retroviral (MSCV) expression of mTagBFP2 fluorescent protein

    Depositor

    Maki Nakayama

    Article

    Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

    Insert

    mTagBFP2
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MCS+

    Plasmid

    #90412

    Purpose

    A FRET sensor that report the electric potential at the inner leaflet of cell plasma membrane

    Depositor

    Katharina Gaus

    Article

    Ma et al Nat Biotechnol. 2017 Mar 13. doi: 10.103

    Insert

    Lck10+ Venus+mcherry+ strech of positively charged amino acids
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMT405

    Plasmid

    #139383

    Purpose

    LacZ, pNBU1 backbone with BsaI sites, AmpR, ErmR

    Depositor

    Christopher Voigt

    Article

    Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

    Insert

    LacZ
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    No promoter

    Available Since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET11b-SpyCatcher-HA2

    Plasmid

    #165991

    Purpose

    SpyCatcher-fused influenza HA ministem with removable HisTag

    Depositor

    Vesa Hytönen

    Article

    Lampinen et al J Nanobiotechnology. 2021 Jan 19;19(1):2

    Insert

    SpyCatcher-HA2
    

    Use

    Tags

    Foldon, HisTag, and SpyCatcher

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMSCVII-E2Crimson

    Plasmid

    #162756

    Purpose

    Retroviral (MSCV) expression of E2-Crimson fluorescent protein

    Depositor

    Maki Nakayama

    Article

    Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

    Insert

    E2-Crimson
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMSCVII-mChe

    Plasmid

    #162753

    Purpose

    Retroviral (MSCV) expression of mCherry fluorescent protein

    Depositor

    Maki Nakayama

    Article

    Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

    Insert

    mCherry
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBb(B5)1k-GFPuv

    Plasmid

    #106393

    Purpose

    IPTG-inducible trc promoter expressing gfpuv on BBR1-B5 origin

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Insert

    GFPuv
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available Since

    March 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAC574

    Plasmid

    #127975

    Purpose

    AMA1 plasmid with hygB selection marker, and USER cassette (PacI/Nt.BbvCI)

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

    Insert

    hygB (hygromycin resistance marker)
    

    Use

    Fungal expression

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Aspergillus nidulans trpC promoter

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pRB035

    Plasmid

    #181944

    Purpose

    aTc-inducible expression of mNeonGreen

    Depositor

    Jeffrey Tabor

    Article

    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

    Insert

    mNeonGreen; tetR (AX04_RS23485 Synthetic)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    mNG-PLtetO-1; tetR-J23106

    Available Since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAAV-UAS-Luciferase

    Plasmid

    #104840

    Purpose

    SPARK reporter gene

    Depositor

    Alice Ting

    Article

    Kim et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

    Insert

    Luciferase
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UAS

    Available Since

    Jan. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. mTagBFP-Lysosomes-20

    Plasmid

    #55263

    Purpose

    Localization: Lysosome Membrane, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    LAMP1
    

    Use

    Tags

    mTagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. pFastBac LIC cloning vector with BioBrick polycistronic restriction sites (11A)

    Plasmid

    #48294

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Nov. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge