We narrowed to 22,140 results for: his

1. pGEXNKI-his-GST-3C-LIC

   Plasmid

   #108709

   Purpose

   Expression of a GST-6xHis-tagged target protein with 3C protease cleavage site.

   Depositor

   Titia Sixma

   Article

   Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

   Type

   Empty backbone

   Use

   Tags

   GST-6xHis-3C protease cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAS1-rab-3P::his-24::tagBFP

   Plasmid

   #124343

   Purpose

   Nuclear Localized Pan-Neuronal tagBFP

   Depositor

   Andrew Leifer

   Article

   Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

   Insert

   rab-3, tagBFP (rab-3 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. SHIP2 WT pBABE-His-Xpress

   Plasmid

   #126584

   Purpose

   Retroviral infection of MEF cells (puromycin selection)

   Depositor

   Christophe Erneux

   Article

   Zhang et al Cell Signal. 2007 Oct;19(10):2194-200. d

   Insert

   SHIP2 (INPPL1 Human)
   

   Use

   Retroviral

   Tags

   His Xpress (N-terminal)

   Expression

   Mutation

   Promoter

   Available Since

   July 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBJG1 T7-8xHis-OGT 5N5A

   Plasmid

   #154280

   Purpose

   Bacterial expression of full length human OGT with 5N5A TPR mutations (inefficient substrate binding). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.

   Depositor

   Suzanne Walker

   Article

   Levine et al J Am Chem Soc. 2018 Mar 14;140(10):3510-

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Tags

   8x His, HRV3C protease site, and T7 leader

   Expression

   Bacterial

   Mutation

   N322A, N356A, N390A, N424A, N458A mutations; bact…

   Promoter

   Available Since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET21a(+)-Histag-Citrine (525)

   Plasmid

   #70718

   Purpose

   Expression vector for Citrine purification

   Depositor

   Richard Murray

   Article

   Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

   Insert

   Citrine
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAS1-rab-3P::his24::tagBFP2

   Plasmid

   #124344

   Purpose

   Nuclear Localized Pan-Neuronal tagBFP2

   Depositor

   Andrew Leifer

   Insert

   rab3, tagBFP2 (rab-3 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA5/FRT/TO HIS DNAJB9

   Plasmid

   #19559

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   DnaJ (Hsp40) homolog, subfamily B, member 9 (DNAJB9 Human)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFA6a-link-yomKO2-SpHis5

   Plasmid

   #44845

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized mKO2 with SpHis5 selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yomKO2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. 6xHis(TEV)-dDrp1-StrepII

   Plasmid

   #174421

   Purpose

   Expresses D. melanogaster Drp1 in bacteria

   Depositor

   Thomas Pucadyil

   Article

   Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

   Insert

   Drp1 (Drp1 Fly)
   

   Use

   Tags

   6xHis(TEV) and StrepII

   Expression

   Bacterial

   Mutation

   None

   Promoter

   T7

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p2CT-His-MBP-Ppr_Cas13a_WT

    Plasmid

    #91872

    Purpose

    Bacterial expression for Cas13a

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

    Insert

    PprCas13a (PprC2c2) WT
    

    Use

    Tags

    His6-MBP-TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHIS3p:mTurquoise2-Tub1+3'UTR::HPH

    Plasmid

    #50629

    Depositor

    Wei-Lih Lee

    Article

    Markus et al Traffic. 2015 Jul;16(7):773-86. doi: 10.

    Insert

    mTurquoise2-Tub1
    

    Use

    Yeast expression

    Tags

    mTurquoise2

    Expression

    Mutation

    Promoter

    HIS3p

    Available Since

    Jan. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-HCMVgH-8his (TB40/E)

    Plasmid

    #136445

    Purpose

    Mammalian expression plasmid for HCMV glycoprotein H (TB40/E strain) extracellular domain with 8his purification tag

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    glycoprotein H (UL75 Human betaherpesvirus 5)
    

    Use

    Tags

    Short GGSG linker and 8his tag

    Expression

    Mammalian

    Mutation

    Extracellular domain (a.a. 1-717). Codon-optimize…

    Promoter

    CMV

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBAD/HisB (TIREVOL)-mEos3.2

    Plasmid

    #189720

    Purpose

    titratable expression of mEos3.2 in bacteria

    Depositor

    Daniel Daley

    Article

    Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

    Insert

    mEos3.2
    

    Use

    Tags

    His-T7-EK

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available Since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a-ClfB_N2N3-HIS-ybbr

    Plasmid

    #101717

    Purpose

    ClfB N2 and N3 domains with C-terminal ybbr tag for covalent immobilization

    Depositor

    Hermann Gaub

    Article

    Milles et al Science. 2018 Mar 30;359(6383):1527-1533

    Insert

    ClfB_N2N3
    

    Use

    Tags

    H3V 3C, HIS, and ybbr

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFA6a-link-yomKeima-SpHis5

    Plasmid

    #44847

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mKeima with SpHis5 selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomKeima
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET21b GFP-Rab10 Q68LHis

    Plasmid

    #186015

    Purpose

    GFP-Rab10 Q68LHis

    Depositor

    Suzanne Pfeffer

    Article

    Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

    Insert

    GFP-Rab10 Q68LHis (RAB10 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PET-B-HypaSpCas9-NLS-6xHis

    Plasmid

    #207386

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HypaSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N692A, M694A, Q695A, H698A, amino acids 1005-1013…

    Promoter

    T7

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET-6xHis-GFP(+36)-DL4

    Plasmid

    #199168

    Purpose

    used to express a supercharged GFP variant that forms inclusion bodies in E. coli

    Depositor

    Allie Obermeyer

    Article

    Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

    Insert

    GFP(+36)-DL4
    

    Use

    Tags

    MGHHHHHHGGA

    Expression

    Mutation

    E16R, T19R, V21K, D29K, E42K, E44K, N49E, D86K, Q…

    Promoter

    T7

    Available Since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. gp64-mVEGF-C-HisTag-pENTR

    Plasmid

    #64065

    Purpose

    Plasmid encodes mature form of murine VEGF-C preceded by baculoviral gp64 secretion signal; entry vector to Gateway cloning system.

    Depositor

    Joanna Bereta

    Article

    Klaus et al Protein Expr Purif. 2015 Mar 7. pii: S10

    Insert

    vascular endothelial growth factor C (Vegfc Mouse)
    

    Use

    Tags

    His-tag

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCB410 (His-Ub-Ate1-4 1A7B)

    Plasmid

    #104339

    Purpose

    Express His Tag Ate1-4 1A7B

    Depositor

    Alexander Varshavsky

    Insert

    ATE1 (Ate1 Mouse)
    

    Use

    Tags

    6xHis-Ub

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRSF_6His-ZmSUMO1a-GG_ZmSCE1b-Myc

    Plasmid

    #91932

    Purpose

    Bacterial expression of maize SUMO1a and SCE1b

    Depositor

    Richard Vierstra

    Article

    Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

    Inserts

    6His-ZmSUMO1a-GG
    ZmSCE1b-Myc
    

    Use

    Tags

    6His and Myc

    Expression

    Bacterial

    Mutation

    Truncation of ZmSUMO1a after the diGlycine motif

    Promoter

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3-ELMOD1-myc-His6

    Plasmid

    #201900

    Purpose

    Used for expression of human protein ELMOD1 in mammalian cells

    Depositor

    Richard Kahn

    Insert

    ELMOD1 (ELMOD1 Human)
    

    Use

    Tags

    myc, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRK5-PLBD2-6xHis S392A

    Plasmid

    #213607

    Purpose

    Expresses PLBD2-6xHis S392A in mammalian cells

    Depositor

    Monther Abu-Remaileh

    Article

    Nyame et al Mol Cell. 2024 Apr 4;84(7):1354-1364.e9.

    Insert

    phospholipase B domain containing 2 (PLBD2 Human)
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Changed serine 392 to alanine

    Promoter

    CMV

    Available Since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1-TRBP-L1+D1+L2-myc/His

    Plasmid

    #180599

    Purpose

    Expressing various parts of TRBP

    Depositor

    Kumiko Ui-Tei

    Article

    Takahashi et al Nucleic Acids Res. 2018 Sep 28;46(17):91

    Insert

    TRBP (TARBP2 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1/NDUFS8/myc-His

    Plasmid

    #127489

    Purpose

    Expresses myc-tagged NDUFS8 in mammalian cells

    Depositor

    Shashi Jain, Dieter Wolf

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFS8 (NDUFS8 Human)
    

    Use

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFA6a-6xGLY-Myc-HIS3MX6

    Plasmid

    #20768

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. p2CT-His-MBP-Lne_Cas13a_WT

    Plasmid

    #91866

    Purpose

    Bacterial expression for Cas13a

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

    Insert

    LneCas13a (LneC2c2) WT
    

    Use

    Tags

    His6-MBP-TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. p2CT-His-MBP-Lbu_C2c2_R1079A_K1080A

    Plasmid

    #91861

    Purpose

    Bacterial expression for Cas13a

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

    Insert

    LbuCas13a (LbuC2c2) R0179A/K1080A
    

    Use

    Tags

    His6-MBP-TEV site

    Expression

    Bacterial

    Mutation

    K1080A

    Promoter

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET23b-Casp9 C287A-His

    Plasmid

    #11830

    Depositor

    Guy Salvesen

    Article

    Stennicke et al J Biol Chem. 1999 Mar 26. 274(13):8359-6

    Insert

    Caspase 9 catalytic mutant (CASP9 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    C287A

    Promoter

    Available Since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

30. pETNKI-his-3C-LIC-kan

    Plasmid

    #108703

    Purpose

    Expression of an His-tagged target protein with 3C protease cleavage site.

    Depositor

    Titia Sixma

    Article

    Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

    Type

    Empty backbone

    Use

    Tags

    His-3C protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pETDuet1-WT-LanCL2-C-Ter-His

    Plasmid

    #154186

    Purpose

    To express LanCL2 in bacterial cells

    Depositor

    Wilfred van der Donk

    Article

    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

    Insert

    LanCL2
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. p2CT-His-MBP-Lbu_C2c2_R472A_H477A

    Plasmid

    #83483

    Purpose

    Bacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 1 inactive mutant).

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

    Insert

    Lbu_C2c2_R472A_H477A
    

    Use

    CRISPR

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    R472A, H477A

    Promoter

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDB.His.MBP.3C CARD8 UPA-CARD WT

    Plasmid

    #164021

    Purpose

    Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    His6-MBP-3C

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pFastBac-His-Flag-Timeless

    Plasmid

    #22892

    Depositor

    Aziz Sancar

    Article

    Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

    Insert

    Timeless (TIMELESS Human)
    

    Use

    Tags

    Flag and His

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

35. pRSF_6His-ZmSUMO1a-GG_ZmSCE1d-Myc

    Plasmid

    #91934

    Purpose

    Bacterial expression of maize SUMO1a and SCE1d

    Depositor

    Richard Vierstra

    Article

    Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

    Inserts

    6His-ZmSUMO1a-GG
    ZmSCE1d-Myc
    

    Use

    Tags

    6His and Myc

    Expression

    Bacterial

    Mutation

    Truncation of ZmSUMO1a after the diGlycine motif

    Promoter

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pnEA-NpM-HsNot4-GB1His6_AG

    Plasmid

    #148792

    Purpose

    Bacterial Expression of HsNot4

    Depositor

    Elisa Izaurralde

    Article

    Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

    Insert

    HsNot4 (CNOT4 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCDFNKI-his-3C-LIC-strep

    Plasmid

    #108705

    Purpose

    Expression of a His-tagged target protein with 3C protease cleavage site.

    Depositor

    Titia Sixma

    Article

    Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

    Type

    Empty backbone

    Use

    Tags

    His-3C protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pBAD/HisB (TIRSTD)-sfGFP

    Plasmid

    #189674

    Purpose

    titratable expression of sfGFP in bacteria

    Depositor

    Daniel Daley

    Article

    Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

    Insert

    sfGFP
    

    Use

    Tags

    His-T7-EK

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available Since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Sup35 ScNM A230C His7

    Plasmid

    #15598

    Depositor

    Susan Lindquist

    Article

    Tessier et al Nature. 2007 May 31. 447(7144):556-61.

    Insert

    Sup35 NM (SUP35 Budding Yeast)
    

    Use

    Tags

    7xHis

    Expression

    Bacterial

    Mutation

    A230C. Alanine 230 mutated to cysteine.

    Promoter

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3-HCMVgO-8his (TB40/E)

    Plasmid

    #136453

    Purpose

    Mammalian expression plasmid for HCMV glycoprotein O (TB40/E strain) with an 8his tag

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    envelope glycoprotein O (UL74 Human betaherpesvirus 5)
    

    Use

    Tags

    Linker and 8his tag (GGSGHHHHHHHH, includes BamHI…

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge