We narrowed to 15,547 results for: pET
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Plasmid#24083DepositorAvailable SinceOct. 27, 2010AvailabilityAcademic Institutions and Nonprofits only
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pET28_6xHis-PARP1delWGR-L713F
Plasmid#173941PurposeBacterial expression of PARP1 lacking WGR domain but containing L713F gain-of-function mutationDepositorInsertPARP1delWGR-L713F (PARP1 Human)
Tags6xHisExpressionBacterialMutationDeletion of residues 521-660; L713F, V762AAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET30a-SRAP-delCys2
Plasmid#84568PurposeEncodes murine SRAP domain (250aa) of Hmces/Srap1 with deletion of catalytic Cys2DepositorInsertSrap1 (Hmces Mouse)
Tags6xHis and S-TagExpressionBacterialMutationdeletion of Cys2PromoterT7Available SinceNov. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
CyPet-A206K-PRL2
Plasmid#149685PurposeFor the FRET-based binding assay between human CNNM3 CBS domain and PRL2.DepositorInsertCyPet-A206K-PRL2
Tags8 histidine tagExpressionBacterialMutationCyPet A206KPromoterT7Available SinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-27
Plasmid#172752PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-27; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-27
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-FPPS
Plasmid#108952Purposeheterologous expression of FPPS of G. stearothermophilus in E. coliDepositorInsertFarnesyldiphosphat synthase (FPPS)
TagsHis6-TagExpressionBacterialAvailable SinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET3-ARL15-His6
Plasmid#201880PurposeUsed for bacterial expression of human protein ARL15DepositorAvailable SinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(R49A)
Plasmid#112726PurposeArg49 of TBP6.7 and other TBPs is the only arginine that was lab-evolved from wild-type U1A to play a significant role in TAR RNA recognition. Mutation results in ~230-fold reduction in Kd.DepositorInsert6His-TEV-TBP6.7(R49A)
Tags6His-TEVExpressionBacterialMutationR49APromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
eGFP-Silicatein-pET28
Plasmid#198012PurposeC-terminal eGFP fusion to truncated silicatein insert with N- and C-terminal hexahistidine tag for purificationDepositorInserteGFP-silicatein
Tagshexahistidine tagExpressionBacterialPromoterT7Available SinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET20b-delta133HtrA2 A141S
Plasmid#16157DepositorAvailable SinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
TrmD_HaeIn/pET28b(+) N,C-tag
Plasmid#12665DepositorInserttRNA (guanine-N(1)-)-methyltransferase (trmD Haemophilus influenzae)
TagsHisExpressionBacterialAvailable SinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET3a-UBE2J1 1-283
Plasmid#185348PurposeIn vitro transcription and translation in bacterial systemDepositorAvailable SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET His10-Src42a
Plasmid#126674PurposeExpression of His10-Src42a for purification from E. coliDepositorInsertSrc42a
ExpressionBacterialAvailable SinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21a-mAIM2-deltaPYD
Plasmid#51535PurposeExpress His6-tagged HIN domain of mAIM2 in bacteriaDepositorAvailable SinceMarch 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-21a(+)-IS200
Plasmid#12604DepositorInsertIS200 transposase (SSO1474 Sulfolobus solfataricus)
ExpressionBacterialAvailable SinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
D5 gene in PETCON vector
Plasmid#87626Purposeexpresses D5 allosteric Zn2+ and fluorescein binding antibody for yeast displayDepositorInsertDesigned allosteric Zn2+ and fluorescein binding antibody scFv D5
Tags3x(G4S) Linker and cMyc tag on backboneExpressionYeastAvailable SinceMarch 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28-p53 (Mouse 1-87)
Plasmid#62061PurposeExpression of Mouse p53 (residues 1-87) in e. coliDepositorInsertp53 (Trp53 Mouse)
TagsHisExpressionBacterialMutationcontains TAD residues 1-87PromoterT7Available SinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
human TLNRD1 pET151
Plasmid#159384PurposeExpresses human TLNRD1 in bacterial cellsDepositorInsertTalin Rod Domain Containing Protein 1 (TLNRD1 Human)
TagsHis-tag, TEV cleavage siteExpressionBacterialPromoterT7Available SinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET30Xa-SBL1(160S)
Plasmid#66872PurposeExpresses WTsoybean lipoxygenase-1 160S with N-term HistagDepositorInsertsoybean lipoxygenase-1 wild type (NEWENTRY Glycine max)
TagsHistag from pET30Xa-LICExpressionBacterialMutationnonePromoterT7lacAvailable SinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-24
Plasmid#172750PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-24; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-24
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-9
Plasmid#172742PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-9; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-9
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
C122-E16: CyPet
Plasmid#162896PurposeStandard Gateway entry clone encoding CyPet fluorescent proteinDepositorInsertCyPet fluorescent protein
UseSynthetic BiologyAvailable SinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
PET28-FnCas12a-EP16
Plasmid#183636PurposePET28-FnCas12a-EP16 can efficiently recognize a broad range of PAM sequences including YN (Y = C or T), TAC and CAA.DepositorInsertFnCas12a-EP16
UseCRISPRTags6×His TagExpressionBacterialMutationN607R, K613V, N617R, K180S, K660R, D616NAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21a-mAIM2-GST
Plasmid#51533PurposeExpress GST-tagged mAIM2 in bacteriaDepositorAvailable SinceMarch 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET20b- hTPI Lys13Arg
Plasmid#50726Purposeoverexpression of the human TPI1 Lys13Arg allele in E.coliDepositorInsertTriosephosphate isomerase 1 (TPI1 Human)
Tags6xHIS tagExpressionBacterialMutationchanged Lysine 13 to ArgeninePromoterT7Available SinceFeb. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-GST-Q11
Plasmid#185790PurposeEncodes glutathione S-transferase linked to Q11 self-assembling peptide via a GS-linker sequenceDepositorInsertGST-Q11
TagsHis-tagExpressionBacterialPromoterT7Available SinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-MBP-creM
Plasmid#112238PurposepET28-derived plasmid that expresses an N-terminal maltose binding fusion construct of CreMDepositorInsertCreM
TagsMBPAvailable SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBC2T-Ypet-FtnA
Plasmid#108969Purposeexpresses BC2-tag-Ypet-FtnA in mammalian cellsDepositorAvailable SinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-8
Plasmid#172761PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-8; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-8
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-5
Plasmid#172760PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-5; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-5
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21b - S. thermophilus DltB
Plasmid#132607Purposeexpresses S. thermophilus DltB in E. coliDepositorInsertDltb
ExpressionBacterialAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETM6-3F2-mCherry
Plasmid#73418PurposeReporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3F2.DepositorInsertPromoter 3F2 (orthogonal T7-lac variant)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterP3F2 (orthogonal T7-lac variant)Available SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
FKBP-YPet-RanGAP*
Plasmid#175245PurposeBacterial expression and purification, SUMOylation substrate that can be recruited to FRB with rapamycin, Ypet is a FRET acceptor for CyPetDepositorAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET151-mUCH37 (ISF2)
Plasmid#61928PurposeExpression of mUCH37 (ISF2) in E.coliDepositorAvailable SinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30a BCP1 NTase
Plasmid#195245PurposeExpresses phage BCP1 NTaseDepositorInsertBCP1 phage NTase
TagsHis6 and SBPExpressionBacterialPromoterT7Available SinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET30a-Av05 NTase
Plasmid#195243PurposeExpresses phage Av05 NTaseDepositorInsertphage Av05 NTase
TagsHis6 and SBPExpressionBacterialPromoterT7Available SinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAR69_STRI_m(KS_MAT)_H8_pET22b
Plasmid#122849PurposeExpresses the condensing part of murine type I fatty acid synthase (FASN) consisting of the KS and MAT domain in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.DepositorInsertm(KS_MAT) (Fasn Mouse)
TagsHis8 tag and Twin-Strep tagExpressionBacterialMutationwildtypePromoterT7 promoterAvailable SinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-BCA-Pep-H
Plasmid#185782PurposeEncodes enzyme bovine carbonic anhydrase linked to Pep-H self-assembling peptide via a GS-linker sequenceDepositorInsertBCA-Pep-H
ExpressionBacterialPromoterT7Available SinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3_YPet-PCP (y-plasmid)
Plasmid#127637PurposeEncodes a fluorecent protein with an RNA binding peptide: YPet-PCP.Expression with constitutive E. coli RNAP promoter (J23106), ribozyme RiboJ and RBS BBa_B0034.DepositorInsertYPet
UseSynthetic BiologyTagsRNA binding peptide: PP7 coat protein (PCP)ExpressionBacterialPromoterpJ21306Available SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-A774L
Plasmid#174796PurposeBacterial expression of a hyperactive PARP1 mutant (A774L destabilizes the autoinhibitory HD subdomain)DepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only