We narrowed to 22,194 results for: tes

1. pRK5-HA-Ubiquitin-K6

   Plasmid

   #121151

   Purpose

   Mammalian expression of HA tagged ubiquitin with K6 only, other lysines mutated to arginines

   Depositor

   Josef Kittler

   Article

   Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

   Insert

   Ubiquitin C (UBC Human)
   

   Expression

   Mammalian

   Mutation

   All Ks apart from K6 mutated to Rs

   Available Since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. NowGFP /pQE-30

   Plasmid

   #74749

   Purpose

   NowGFP gene for bacterial expression

   Depositor

   Konstantin Lukyanov

   Article

   Sarkisyan et al Biophys J. 2015 Jul 21;109(2):380-9. doi

   Insert

   NowGFP
   

   Tags

   HisTag (with a mutation but still working)

   Expression

   Bacterial

   Mutation

   WasCFP with substitutions E6K, L42M, T43S, V68M, …

   Promoter

   T5

   Available Since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDRM166 LKB (Luciferase mKate2 Blast)

   Plasmid

   #183502

   Purpose

   Expresses the firefly luciferase gene, the NLS-tagged mKate2 gene, and the blasticidin resistance gene joined by P2A linkers.

   Depositor

   Wayne Tilley

   Article

   Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

   Inserts

   Firefly Luciferase
   mKate2
   BSD
   

   Use

   Lentiviral and Luciferase

   Tags

   P2A linker and SV40 NLS with GGS linker

   Expression

   Mammalian

   Promoter

   MND, MND (off Luc-P2A), and MND (off Luc-P2A-mKat…

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-K-GECO1

   Plasmid

   #105865

   Purpose

   Mammalian cell expression plasmid for red fluorescent Ca2+ indicator K-GECO1

   Depositor

   Robert Campbell

   Article

   Shen et al BMC Biol. 2018 Jan 16;16(1):9. doi: 10.1

   Insert

   K-GECO1
   

   Expression

   Mammalian

   Available Since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDuEx2-NIR-GECO1

   Plasmid

   #113680

   Purpose

   Bacterial and mammalian expression plasmid for near-infrared fluorescent Ca2+ indicator NIR-GECO1

   Depositor

   Robert Campbell

   Article

   Qian et al Nat Methods. 2019 Feb;16(2):171-174. doi

   Insert

   NIR-GECO1
   

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRK5-HA-Ubiquitin-K11

   Plasmid

   #121152

   Purpose

   Mammalian expression of HA tagged ubiquitin with K11 only, other lysines mutated to arginines

   Depositor

   Josef Kittler

   Article

   Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

   Insert

   Ubiquitin C (UBC Human)
   

   Expression

   Mammalian

   Mutation

   All Ks apart from K11 mutated to Rs

   Available Since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOPINK-OTUD4 (OTU, aa 22-196)

   Plasmid

   #61413

   Purpose

   Expresses human OTUD4 (OTU domain) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUD4 (OTU domain-containing protein 4) (OTUD4 Human)
   

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-21 and aa 197-1114.

   Promoter

   T7

   Available Since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDRM18 LTN (Luciferase tdTomato Neo)

   Plasmid

   #174721

   Purpose

   Expresses the firefly luciferase gene, E2A, tdTomato, P2A, and the neomycin resistance gene.

   Depositor

   Wayne Tilley

   Article

   Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

   Inserts

   Firefly Luciferase
   tdTomato
   aph
   

   Use

   Lentiviral and Luciferase

   Tags

   E2A linker and P2A linker

   Expression

   Mammalian

   Promoter

   MND, MND (off Luc-E2A), and MND (off Luc-E2A-tdTo…

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBAD-K-GECO1

   Plasmid

   #105864

   Purpose

   Bacterial expression plasmid for red fluorescent Ca2+ indicator K-GECO1

   Depositor

   Robert Campbell

   Article

   Shen et al BMC Biol. 2018 Jan 16;16(1):9. doi: 10.1

   Insert

   K-GECO1
   

   Tags

   6-His Tag

   Expression

   Bacterial

   Available Since

   April 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRK5-HA-Ubiquitin-K11R

    Plasmid

    #121154

    Purpose

    Mammalian expression of HA tagged ubiquitin K11R

    Depositor

    Josef Kittler

    Article

    Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

    Insert

    Ubiquitin C (UBC Human)
    

    Expression

    Mammalian

    Mutation

    K11 mutated to an R

    Available Since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. Ratiometric FPX sensor for ERK kinase activity

    Plasmid

    #60974

    Purpose

    For imaging of ERK kinase activity using a single polypeptide RA-WW domain-ERK substrate-B protein and free GA-NES.

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddRFP A-WW domain-ERK substrate-ddFP B
    

    Tags

    ERK substrate is before ddFP copy B, WW domain is…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pRK5-HA-Ubiquitin-K27R

    Plasmid

    #121155

    Purpose

    Mammalian expression of HA tagged ubiquitin K27R

    Depositor

    Josef Kittler

    Article

    Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

    Insert

    Ubiquitin C (UBC Human)
    

    Expression

    Mammalian

    Mutation

    K27 mutated to an R

    Available Since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. RANLS-DEVD-BNES

    Plasmid

    #50840

    Purpose

    Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-3 biosensor, used together with plasmid 2 (BNLS).

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddRFP A and ddRFP B
    

    Tags

    NES sequence LALKLAGLDIGS and triplicated NLS seq…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRK5-HA-Ubiquitin-K6R

    Plasmid

    #121153

    Purpose

    Mammalian expression of HA tagged ubiquitin K6R

    Depositor

    Josef Kittler

    Article

    Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

    Insert

    Ubiquitin C (UBC Human)
    

    Expression

    Mammalian

    Mutation

    K6 mutated to an R

    Available Since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. single polypeptide FPX biosensor for caspase-1

    Plasmid

    #60885

    Purpose

    FPX biosensor for caspase-1

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    single polypeptide FPX biosensor for caspase-1
    

    Tags

    HindIII site and NES sequence LALKLAGLDIGS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

16. GANES-DEVD-BNLS

    Plasmid

    #50842

    Purpose

    Expresses a tandem green ddFP heterodimer in mammalian cells, construct 1 for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with a second plasmid RANLS.

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddGFP A and ddGFP B
    

    Tags

    NES sequence LALKLAGLDIGS placed after ddGFP A an…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRK5 FLAG 17xS/T->A, catalytic active lipin 1

    Plasmid

    #32007

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2011 Aug 5;146(3):408-20.

    Insert

    lipin1 (Lpin1 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S106A, S150A, (S281A, T282A), S285A, S287A, S293A…

    Promoter

    CMV, SP6

    Available Since

    Oct. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pTwist-WIV1-CoV Δ18

    Plasmid

    #164439

    Purpose

    Encodes WIV1-CoV Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

    Insert

    WIV1-CoV Spike truncated to remove 18 amino acids
    

    Expression

    Mammalian

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. NESRA-DEVD-BNLS

    Plasmid

    #50849

    Purpose

    Expresses a tandem red ddFP heterodimer in mammalian cells, construct 1 for a red to green colour switch-based fluorescent caspase-3 biosensor, used together with a second plasmid GANLS.

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddRFP A and ddRFP B
    

    Tags

    NES sequence LQKKLEELELDE placed after ddGFP A an…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. single polypeptide FPX biosensor for caspase-8

    Plasmid

    #60884

    Purpose

    FPX biosensor for caspase-8

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    single polypeptide FPX biosensor for caspase-8
    

    Tags

    HindIII site and NES sequence LALKLAGLDIGS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only