165,775 results

1. pEGFP-IQGAP1

   Plasmid

   #30112

   Depositor

   David Sacks

   Article

   Ren et al J Biol Chem. 2005 Oct 14. 280(41):34548-

   Insert

   IQGAP1 (IQGAP1 Human)
   

   Tags

   tandem EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX458-Ef1a-Cas9-H2B-mCherry

   Plasmid

   #159655

   Purpose

   Empty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B mCherry nuclear reporter.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCI-Neo HA-hSnoN

   Plasmid

   #10908

   Depositor

   Bob Weinberg

   Article

   Sun et al Proc Natl Acad Sci U S A. 1999 Oct 26. 9

   Insert

   SnoN (SKIL Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pDIV270

   Plasmid

   #226181

   Purpose

   Expresses Cas9 and gRNA targeting KU70 gene in K. phaffii.

   Depositor

   Uffe Mortensen

   Article

   Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

   Insert

   tRNA-sgRNA-tRNA
   

   Use

   CRISPR

   Expression

   Bacterial and Yeast

   Promoter

   TEF1p (Komagataella phaffii)

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMGF196

   Plasmid

   #97005

   Purpose

   Expresses LEMD2-mCherry in mammalian cells under a crippled promoter

   Depositor

   Adam Frost, Wesley Sundquist

   Article

   Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

   Insert

   LEMD2 (LEMD2 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV delta 5

   Available Since

   Aug. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. plRL19

   Plasmid

   #163634

   Purpose

   L5 Integrase delivery plasmid to allow integration of L5 attP only plasmids (e.g. plRL61, ID #163633).

   Depositor

   Jeremy Rock

   Article

   Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

   Insert

   L5 Integrase
   

   Expression

   Bacterial

   Promoter

   Mycobacterial Optimized Promoter (MOP) and Tet-On

   Available Since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFPc1 IP3R3 WT

   Plasmid

   #236453

   Purpose

   transient overexpression of IP3R3 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Kuchay et al Nature. 2017 Jun 22;546(7659):554-558. d

   Insert

   IP3R3 (Itpr3 Rat)
   

   Expression

   Mammalian

   Available Since

   May 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. RfxCas13d-backbone

   Plasmid

   #165078

   Purpose

   Expression of RfxCas13d sgRNAs from a U6 promoter

   Depositor

   Ling-Ling Chen

   Article

   Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

   Insert

   Scaffold gRNA
   

   Expression

   Mammalian

   Available Since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST40-Arl13b11

   Plasmid

   #40873

   Depositor

   Tamara Caspary

   Article

   Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

   Insert

   Arl13b (Arl13b Mouse)
   

   Tags

   His and V5

   Expression

   Mammalian

   Mutation

   no stop codon

   Promoter

   CMV

   Available Since

   Nov. 5, 2012

   Availability

   Academic Institutions and Nonprofits only

10. SuExp His-Myc-hPLD3

    Plasmid

    #173851

    Purpose

    Protein expression plasmid for recombinant His-Myc tag human PLD3

    Depositor

    David Nemazee

    Article

    Gavin et al Nat Commun. 2021 Oct 7;12(1):5874. doi:

    Insert

    PLD3 (PLD3 Human)
    

    Tags

    6xHis, Myc, Factor X cleavable

    Expression

    Mammalian

    Mutation

    intracellular and transmembrane domain truncated,…

    Promoter

    CMV

    Available Since

    Jan. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-EF1a-fDIO-Cre

    Plasmid

    #121675

    Purpose

    Expresses Cre recombinase and HA tag in a Flp dependent fashion (fDIO)

    Depositor

    Esteban Engel, Alexander Nectow

    Article

    Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30

    Has Service

    AAV Retrograde, AAV1, AAV5, AAV8, and AAV9

    Insert

    NLS-CRE-HA
    

    Use

    AAV

    Tags

    3xHA and SV40-NLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHLmMBP-10

    Plasmid

    #72348

    Purpose

    Mammalian expression of secreted N-terminally 8His-tagged mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

    Depositor

    Luca Jovine

    Article

    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

    Type

    Empty backbone

    Tags

    6His-tag, 8His-tag, HA-tag, Strep-tag II, and mMB…

    Expression

    Mammalian

    Promoter

    CMV enhancer + chicken beta-actin promoter

    Available Since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. TFORF0985

    Plasmid

    #142249

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    KLF4 (KLF4 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pmCherry-PH(Akt2)

    Plasmid

    #86596

    Purpose

    Expresses mCherry-tagged PH(WT) domain of human Akt2

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt2 PH domain (AKT2 Human)
    

    Expression

    Mammalian

    Mutation

    Amino acids 1 - 108

    Available Since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.2/EV

    Plasmid

    #120833

    Purpose

    Control vector, 20nt random sequence between gateway recombination sites

    Depositor

    Jan Rehwinkel

    Article

    Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. TIGRE docking site targeting vector

    Plasmid

    #61580

    Purpose

    Create a docking site in the mouse TIGRE genomic locus that allows modification in ES cells by Flp recombinase-mediated cassette exchange

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Type

    Empty backbone

    Use

    Mouse Targeting

    Available Since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pmCherry-ATG5

    Plasmid

    #13095

    Depositor

    Roberta Gottlieb

    Article

    Hamacher-Brady et al Cell Death Differ. 2007 Jan;14(1):146-57

    Insert

    ATG5 (Atg5 Mouse)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Nov. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHM840

    Plasmid

    #20701

    Depositor

    Thomas Stamminger

    Article

    Sorg et al Biotechniques. 1999 May . 26(5):858-62.

    Insert

    GFP-NLS-lacZ
    

    Expression

    Mammalian

    Available Since

    April 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA-NES-PhoCl2f-mCherry

    Plasmid

    #164048

    Purpose

    Optogenetic control of protein localization by PhoCl2f

    Depositor

    Robert Campbell

    Article

    Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

    Insert

    NES-PhoCl2f-mCherry
    

    Tags

    kozak sequence

    Expression

    Mammalian

    Promoter

    CMV promotor

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. qTAG-C-Solo-mStayGold-LAMP1

    Plasmid

    #227322

    Purpose

    Donor template for mStayGold insertion into the C-terminus of the LAMP1 locus. For lysosome visualization. To be co-transfected with sgRNA plasmid px330-LAMP1 (Addgene #207787)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    LAMP1 Homology Arms flanking a mStayGold Tag (LAMP1 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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