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Showing: 2281 - 2320 of 26021 results

  3. mAIRN HO

    Plasmid
    #207411
    Purpose
    Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).
    Depositor
    Karlene Cimprich
    Article
    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do
    Insert
    mAIRN
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    207411-plasmid-map-sequence-id-422696

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  4. mAIRN CD

    Plasmid
    #207412
    Purpose
    Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).
    Depositor
    Karlene Cimprich
    Article
    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do
    Insert
    mAIRN
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    207412-plasmid-map-sequence-id-422689

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  9. pL0-MtPT4Pro

    Plasmid
    #159414
    Purpose
    Medicago truncatula PT4 promoter sequence (836 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.
    Depositor
    Sam Brockington
    Article
    Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d
    Insert
    MtPT4 Promoter
    Use
    Puc19-derived
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    159414-plasmid-map-sequence-id-323169

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  10. pL0-MtBCP1Pro

    Plasmid
    #159415
    Purpose
    Medicago truncatula BCP1 promoter sequence (1108 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.
    Depositor
    Sam Brockington
    Article
    Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d
    Insert
    MtBCP1 Promoter
    Use
    Puc19-derived
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    159415-plasmid-map-sequence-id-323165

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  11. IMS-HyPer2

    Plasmid
    #60248
    Purpose
    Genetically-encoded fluorescent hydrogen peroxide indicator HyPer2 targeted to mitochondrial intermembrane spase.
    Depositor
    Vsevolod Belousov
    Article
    Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.
    Insert
    IMS-HyPer2
    Use
    Tags
    Mitochondrial intermembrane space targeting signa…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    60248-plasmid-map-sequence-id-317297

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  12. pFEndoNA

    Plasmid
    #174724
    Purpose
    Expresses GFP-endoNA fusion protein for degradation of polysialic acid. The endoNA is a catalytically active endosialidase from bacteriophage PK1A.
    Depositor
    Jukka Finne
    Article
    Jokilammi et al J Immunol Methods. 2004 Dec;295(1-2):149
    Insert
    GFP-endoNA fusion protein
    Use
    Tags
    Histag
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    Sept. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    174724-plasmid-map-sequence-id-343066

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  14. pDR-GFPuniv

    Plasmid
    #46085
    Purpose
    In vivo recombination assay fluorescent reporter
    Depositor
    Raymond Monnat
    Article
    Li et al Nucleic Acids Res. 2012 Mar;40(6):2587-9
    Insert
    DR-GFPuniv reporter
    Use
    Recombination reporter plasmid
    Tags
    Expression
    Mammalian
    Mutation
    E5G and K163R mutations in EGFP relative to wild-…
    Promoter
    Available since
    May 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    46085-plasmid-map-sequence-id-64906

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  16. ECFP HO

    Plasmid
    #207413
    Purpose
    Mammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).
    Depositor
    Karlene Cimprich
    Article
    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do
    Insert
    ECFP
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Jan. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    207413-plasmid-map-sequence-id-421043

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  17. ECFP CD

    Plasmid
    #207414
    Purpose
    Mammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).
    Depositor
    Karlene Cimprich
    Article
    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do
    Insert
    ECFP
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Jan. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    207414-plasmid-map-sequence-id-421036

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  19. pETcLIC-GFP

    Plasmid
    #183514
    Purpose
    The plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in E. coli
    Depositor
    Martin Högbom
    Article
    Grave et al Protein Expr Purif. 2022 Oct;198:106132.
    Type
    Empty backbone
    Use
    Tags
    10x His-tag and folding reporter GFP
    Expression
    Bacterial
    Mutation
    Promoter
    T7 lac
    Available since
    June 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    183514-plasmid-map-sequence-id-362488

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  21. SP6-VEE-IRES-Puro

    Plasmid
    #58971
    Purpose
    noninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon
    Depositor
    Steven Dowdy
    Article
    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.
    Type
    Empty backbone
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    26S subgenomic promoter
    Available since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    58971-plasmid-map-sequence-id-91402

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  26. pGL3

    Plasmid
    #48743
    Purpose
    Expression of luciferase driven by truncated mPer2 promoter region (bases -1128 to +1060 with respect to transcription start site at +1)
    Depositor
    Joseph Takahashi
    Article
    Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10
    Insert
    truncated mPer2 promoter/enhancer region (-1128 to +1060)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    truncated promoter region (see pGL6)
    Promoter
    Available since
    Dec. 19, 2013
    Availability
    Academic Institutions and Nonprofits only

  27. pL0_AC-CEN_ARS_HIS

    Plasmid
    #202129
    Purpose
    Level 0 plasmid containing a centromere sequence that is recognized in diatoms with AC overhangs used to build level 1 constructs.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    CEN_ARS_HIS
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    ARS sequence mutated to remove sapI site for dome…
    Promoter
    Available since
    Aug. 25, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202129-plasmid-map-sequence-id-405565

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  32. pT2SCb

    Plasmid
    #59385
    Purpose
    Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a modified chloramphenicol resistance gene.
    Depositor
    Shelley Copley
    Article
    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.
    Insert
    TT-ISceI-cat2
    Use
    Template
    Tags
    Expression
    Mutation
    The 3’-end of the chloramphenicol resistance gene…
    Promoter
    Available since
    Sept. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    59385-plasmid-map-sequence-id-92126

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  39. pGL2

    Plasmid
    #48742
    Purpose
    Expression of luciferase driven by truncated mPer2 promoter region (bases -1128 to +386 with respect to transcription start site at +1)
    Depositor
    Joseph Takahashi
    Article
    Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10
    Insert
    truncated mPer2 promoter/enhancer region (-1128 to +386)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    truncated promoter region (see pGL6)
    Promoter
    Available since
    Dec. 19, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 2281 - 2320 of 26021 results