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Addgene

We narrowed to 8,814 results for: CAG

Showing: 2851 - 2900 of 8814 results
  1. pLV(gRNA)-CMV-eGFP-U6(sgCTG)

    Plasmid
    #216732
    Purpose
    Contains a eGFP gene along with a U6 promoter driving the sgCTG (target sequence: (CUG)6). Used with the HD iPSC-derived astrocytes
    Depositor
    Insert
    sgCTG lentiviral guide RNA with eGFP tag
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and U6
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
  2. PB-TRE3G-dN40-TP53-CCRB

    Plasmid
    #236774
    Purpose
    A piggybac-based cloning vector containing TRE3G promoter-driven short-form of TP53 and CAG promoter-driven nuclear-localized Clover-P2A-rtTA-IRES-BSD.
    Insert
    TP53 (TP53 Human)
    Use
    Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    deleted amino acids 1-40
    Promoter
    TRE3G promoter and TRE3G promoter, CAG promoter
    Available since
    May 29, 2025
    Availability
    Academic Institutions and Nonprofits only
  3. PB-TRE3G-TP53-CCRB

    Plasmid
    #236773
    Purpose
    A piggybac-based cloning vector containing TRE3G promoter-driven full-length TP53 and CAG promoter-driven nuclear-localized Clover-P2A-rtTA-IRES-BSD.
    Insert
    TP53 (TP53 Human)
    Use
    Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G promoter and TRE3G promoter, CAG promoter
    Available since
    May 29, 2025
    Availability
    Academic Institutions and Nonprofits only
  4. PB-U6-CNCB_sgEIF3D-4

    Plasmid
    #236766
    Purpose
    A piggybac-based vector containing mouse U6 promoter-driven EIF3D sgRNA #4 and CAG promoter-driven nuclear-localized Clover-P2A-BSR.
    Insert
    EIF3D sgRNA-4 (EIF3D Human)
    Use
    Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    mouse U6 promoter and mouse U6 promoter, CAG prom…
    Available since
    May 22, 2025
    Availability
    Academic Institutions and Nonprofits only
  5. pAAV-U6-sgCTG-CMV-GFP

    Plasmid
    #216734
    Purpose
    AAV plasmid containing sgCTG (target sequence: (CUG)6) driven by a U6 promoter and eGFP under the control of the CMV promoter. Used for in vivo experiments.
    Depositor
    Insert
    sgCTG
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and CMV
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
  6. Syn-FLEX-MCS-WPRE-SV40 - GCH8

    Plasmid
    #230818
    Purpose
    AAV cloning vector for CRE dependent expression under the Syn promoter.
    Depositor
    Type
    Empty backbone
    Use
    AAV and Cre/Lox; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
  7. EF1a-Flex-MCS-WPRE-SV40 - GCH14

    Plasmid
    #230821
    Purpose
    AAV cloning vector for CRE dependent expression under the EF1a promoter.
    Depositor
    Type
    Empty backbone
    Use
    AAV and Cre/Lox; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
  8. GFAP-Flex-MCS-WPRE-SV40 - GCH32

    Plasmid
    #230830
    Purpose
    AAV cloning vector for CRE dependent expression under the GFAP promoter.
    Depositor
    Type
    Empty backbone
    Use
    AAV and Cre/Lox; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
  9. TRE-Flex-MCS-WPRE-SV40 - GCH26

    Plasmid
    #230827
    Purpose
    AAV cloning vector for CRE dependent expression under the TRE promoter.
    Depositor
    Type
    Empty backbone
    Use
    AAV and Cre/Lox; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
  10. CaMKII-Flex-MCS-WPRE-SV40 - GCH20

    Plasmid
    #230824
    Purpose
    AAV cloning vector for CRE dependent expression under the CaMKII promoter.
    Depositor
    Type
    Empty backbone
    Use
    AAV and Cre/Lox; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
  11. IGI-P0492 pHR-dCas9-NLS-VPR-mCherry

    Plasmid
    #102245
    Purpose
    Lentiviral expression of dCas9-VPR-mCherry fusion protein for CRISPRa.
    Depositor
    Insert
    dCas9-VPR-mCherry
    Use
    CRISPR and Lentiviral
    Tags
    NLS-VPR-mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
  12. pmCellFree_KA1_SARS-COV-2_NSP2

    Plasmid
    #169377
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP2 with N-term eGFP
    Depositor
    Insert
    SARS-COV-2 _NSP2
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    June 16, 2021
    Availability
    Academic Institutions and Nonprofits only
  13. pmCellFree_KA1_SARS-COV-2_NSP3

    Plasmid
    #169378
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP3 with N-term eGFP
    Depositor
    Insert
    SARS-COV-2 _NSP3
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    June 16, 2021
    Availability
    Academic Institutions and Nonprofits only
  14. pmCellFree_KA1_SARS-COV-2_ORF9b

    Plasmid
    #169396
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF9bwith N-term eGFP
    Depositor
    Insert
    SARS-COV-2 _ORF9b
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
  15. pmCellFree_KA1_SARS-COV-2_NSP9

    Plasmid
    #169383
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP9 with N-term eGFP
    Depositor
    Insert
    SARS-COV-2 _NSP9
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    July 2, 2021
    Availability
    Academic Institutions and Nonprofits only
  16. pmCellFree_KA1_SARS-COV-2_NSP12

    Plasmid
    #169385
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP12 with N-term eGFP
    Depositor
    Insert
    SARS-COV-2 _NSP12
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
  17. pmCellFree_KA1_SARS-COV-2_NSP15

    Plasmid
    #169388
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP15 with N-term eGFP
    Depositor
    Insert
    SARS-COV-2 _NSP15
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    July 2, 2021
    Availability
    Academic Institutions and Nonprofits only
  18. pmCellFree_KA1_SARS-COV-2_NSP5

    Plasmid
    #169380
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP5 with N-term eGFP
    Depositor
    Insert
    SARS-COV-2 _NSP5
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    June 16, 2021
    Availability
    Academic Institutions and Nonprofits only
  19. pmCellFree_KA1_SARS-COV-2_NSP4

    Plasmid
    #169379
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP4 with N-term eGFP
    Depositor
    Insert
    SARS-COV-2 _NSP4
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    Aug. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
  20. pmCellFree_KA1_SARS-COV-2_NSP8

    Plasmid
    #169382
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP8 with N-term eGFP
    Depositor
    Insert
    SARS-COV-2 _NSP8
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    July 2, 2021
    Availability
    Academic Institutions and Nonprofits only
  21. pmCellFree_KA1_SARS-COV-2_NSP13

    Plasmid
    #169386
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP13 with N-term eGFP
    Depositor
    Insert
    SARS-COV-2 _NSP13
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    July 2, 2021
    Availability
    Academic Institutions and Nonprofits only
  22. pmCellFree_KA1_SARS-COV-2_ORF9c

    Plasmid
    #169397
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF9c with N-term eGFP
    Depositor
    Insert
    SARS-COV-2 _ORF9c
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    July 2, 2021
    Availability
    Academic Institutions and Nonprofits only
  23. pmCellFree_KA1_SARS-COV-2_NSP14

    Plasmid
    #169387
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP14 with N-term eGFP
    Depositor
    Insert
    SARS-COV-2 _NSP14
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
  24. pmCellFree_KA1_SARS-COV-2_NSP7

    Plasmid
    #169381
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP7 with N-term eGFP
    Depositor
    Insert
    SARS-COV-2 _NSP7
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
  25. miABE

    Plasmid
    #205412
    Purpose
    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Insert
    pU6-_RNA*-pCAG-bpNLS-TadA8e(V106W)-32aa-OgeuIscB*(D61A)-GS-bpNLS-GS-TadA8e(V106W)-bpNLS-bGH polyA-pCMV-mCherry-AmpR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    D61A+E85R+H369R+S387R+S457R
    Promoter
    hU6, CAG, CMV
    Available since
    Dec. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
  26. pX551-miniCMV-SpCas9D10A nickase

    Plasmid
    #216735
    Purpose
    Expresses SpCas9-D10A nickase from a miniCMV promoter. For AAV packaging. Derived from pX551-miniCMV-SpCas9, which was a gift from Alex Hewitt (Addgene #107031)
    Depositor
    Insert
    Cas9-D10A nickase
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    T7 and mini-CMV
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
  27. PB-2G-CNCB_sgRBBP6-68

    Plasmid
    #237555
    Purpose
    A piggybac-based vector containing mouse U6 promoter-driven RBBP6 sgRNA #6, human U6 promoter-driven RBBP6 sgRNA #8 and CAG promoter-driven nuclear-localized Clover-T2A-BSR.
    Insert
    RBBP6 (RBBP6 Human)
    Use
    Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    mouse U6 promoter and mouse U6 promoter, human U6…
    Available since
    May 21, 2025
    Availability
    Academic Institutions and Nonprofits only
  28. pAAV-nEF-SpCas9D10A nickase

    Plasmid
    #216736
    Purpose
    Expresses SpCas9-D10A nickase from a nEF promoter. For AAV packaging. Derived from pAAV-nEF-SpCas9 (Addgene #87115)
    Depositor
    Insert
    Cas9-D10A nickase
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    EF-1-alpha core
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
  29. pAAV-FLEX-tdTomato

    Plasmid
    #28306
    Depositor
    Has Service
    AAV PHP.S, AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    tdTomato
    Use
    AAV and Cre/Lox; Adeno-associated virus
    Tags
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CAG
    Available since
    Sept. 7, 2012
    Availability
    Academic Institutions and Nonprofits only
  30. pSaCas9_GFP

    Plasmid
    #64709
    Purpose
    Co-expression of human codon-optimized Staphylococcus aureus Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells and other mammalian cells
    Depositor
    Insert
    SaCas9-2A-GFP
    Use
    CRISPR
    Tags
    2A-GFP and 3xFLAG-SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only
  31. pmCellFree_KA1_SARS-COV-2_NSP16

    Plasmid
    #169389
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP16 with N-term eGFP
    Depositor
    Insert
    SARS-COV-2 _NSP16
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
  32. pmCellFree_KA1_SARS-COV-2_NSP10

    Plasmid
    #169384
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP10 with N-term eGFP
    Depositor
    Insert
    SARS-COV-2 _NSP10
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
  33. pmCellFree_KA1_SARS-COV-2_ORF3b

    Plasmid
    #169391
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF3b with N-term eGFP
    Depositor
    Insert
    SARS-COV-2 _ORF3b
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
  34. TK4-NLS-EGFP

    Plasmid
    #239046
    Purpose
    PiggyBac plasmid for stable transgene expression during human pluripotent stem cell differentiation
    Depositor
    Inserts
    EGFP
    puroR-T2A-mycNLS-mTagBFP2
    Use
    Tags
    T2A-mycNLS-mTagBFP2 and c-myc-NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and EF1a
    Available since
    May 20, 2025
    Availability
    Academic Institutions and Nonprofits only
  35. pcDNA-rUSP19ER-siRNAresistant

    Plasmid
    #155251
    Purpose
    for mammalian overexpression of the siRNA resistant ER isoform of rat USP19
    Depositor
    Insert
    USP19 (ER isoform) siRNA resistant (Usp19 Rat)
    Use
    Tags
    6XHis-3XFlag
    Expression
    Mammalian
    Mutation
    Lys(23)AAG to AAA, Lys(24)AAG to AAA, Gln(26)CAG …
    Promoter
    CMV
    Available since
    Oct. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
  36. pcDNA-rUSP19CYT-siRNAresistant

    Plasmid
    #155252
    Purpose
    for mammalian overexpression of the siRNA resistant CYT isoform of rat USP19
    Depositor
    Insert
    USP19 (CYT isoform) siRNA resistant (Usp19 Rat)
    Use
    Tags
    6XHis-3XFlag
    Expression
    Mammalian
    Mutation
    Lys(23)AAG to AAA, Lys(24)AAG to AAA, Gln(26)CAG …
    Promoter
    CMV
    Available since
    Oct. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
  37. pmCellFree_KA1_SARS-COV-2_S

    Plasmid
    #169402
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus S with N-term eGFP
    Depositor
    Insert
    SARS-COV-2 _S (S SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    June 29, 2021
    Availability
    Academic Institutions and Nonprofits only
  38. pmCellFree_KA3_SARS-COV-2_RBD

    Plasmid
    #169405
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 RBD domain of S protein with C-term mCherry
    Depositor
    Insert
    SARS-COV-2 _RBD (S SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    July 2, 2021
    Availability
    Academic Institutions and Nonprofits only
  39. pmCellFree_KA1_SARS-COV-2_ORF10

    Plasmid
    #169398
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF10 with N-term eGFP
    Depositor
    Insert
    SARS-COV-2 _ORF10 (ORF10 SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    July 2, 2021
    Availability
    Academic Institutions and Nonprofits only
  40. pmCellFree_KA1_SARS-COV-2_M

    Plasmid
    #169400
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus M with N-term eGFP
    Depositor
    Insert
    SARS-COV-2 _M (M SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    July 2, 2021
    Availability
    Academic Institutions and Nonprofits only
  41. px330_SMAD exon 2 gRNA

    Plasmid
    #68350
    Purpose
    px330 with gRNA towards SMAD exon 2. Cas9 is expressed from a CAG promoter.
    Insert
    SMAD exon 2 gRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
  42. pmCellFree_KA1_SARS-COV-2_E

    Plasmid
    #169399
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus E with N-term eGFP
    Depositor
    Insert
    SARS-COV-2 _E (E SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
  43. pmCellFree_KA1_SARS-COV-2_ORF8

    Plasmid
    #169395
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF8 with N-term eGFP
    Depositor
    Insert
    SARS-COV-2 _ORF8 (ORF8 SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    June 29, 2021
    Availability
    Academic Institutions and Nonprofits only
  44. px330_P53 exon 8 gRNA

    Plasmid
    #68349
    Purpose
    px330 with gRNA towards P53 exon 8. Cas9 is expressed from a CAG promoter.
    Insert
    P53 exon 8 gRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 2851 - 2900 of 8814 results