159,557 results

1. N-BLa 1.2

   Plasmid

   #88999

   Purpose

   BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the N-terminal fragment of the split β-lactamase.

   Depositor

   Dieter Langosch

   Article

   Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

   Insert

   N-BLa 1.2 fusion protein
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. C-BLa 1.2

   Plasmid

   #89000

   Purpose

   BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the C-terminal fragment of the split β-lactamase.

   Depositor

   Dieter Langosch

   Article

   Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

   Insert

   C-BLa 1.2 fusion protein
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. SELP-bio-His

   Plasmid

   #51639

   Purpose

   Expresses full-length P-selectin ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   SELP (SELP Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. 3XFlagNICD1

   Plasmid

   #20183

   Depositor

   Raphael Kopan

   Article

   Ong et al J Biol Chem. 2006 Feb 24. 281(8):5106-19

   Insert

   Notch 1 Intracellular Domain (Notch1 Mouse)
   

   Use

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Contains murine Notch 1 Intracellular Domain (Val…

   Promoter

   CMV

   Available since

   Dec. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti6.2-ccdB-3xFLAG-V5

   Plasmid

   #87071

   Purpose

   Lentiviral Gateway-compatible expression vector backbone with a C-terminal 3xFLAG-V5 tag

   Depositor

   Mikko Taipale, Susan Lindquist

   Article

   Taipale et al Cell. 2014 Jul 17;158(2):434-48. doi: 10

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3xFLAG-V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEMB52/14-3-3_1_247

   Plasmid

   #40541

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   YWHAG (YWHAG Human)
   

   Use

   Baculovirus

   Tags

   6xHis

   Expression

   Mutation

   WT; aa1-247

   Promoter

   polH

   Available since

   Oct. 1, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. PSD-95-pTagRFP

   Plasmid

   #52671

   Purpose

   expression of TagRFP-tagged PSD-95

   Depositor

   Johannes Hell

   Insert

   PSD-95 (Dlg4 Rat)
   

   Use

   Tags

   tagRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. CAG FLEX BARK D110A p2A mCherry

   Plasmid

   #117694

   Purpose

   CAG-FLEx-iBARK(D110A)-p2A-mCherry: Negative control viral expression vector for Cre-dependent Gaq silencing

   Depositor

   Baljit Khakh

   Article

   Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

   Insert

   BARKrgs D110A peptide
   

   Use

   Cre/Lox

   Tags

   HA / FLAG

   Expression

   Mutation

   D110A

   Promoter

   Available since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. loxP-Hygro-loxP-HA-mStayGold-Rab11 HR

   Plasmid

   #229678

   Purpose

   Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with monomeric StayGold and an HA epitope tag. Contains a hygromycin resistance cassette for selection of edited cells

   Depositor

   Francesca Bottanelli

   Article

   Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

   Insert

   genomic sequence Rab11 locus (RAB11A Human)
   

   Use

   CRISPR

   Tags

   monomeric StayGold, HA

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. mPA-GFP-Calnexin-N-14

    Plasmid

    #57122

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 400 / 504, Emission: 515 / 517

    Depositor

    Michael Davidson

    Insert

    Calnexin (CANX Human)
    

    Use

    Tags

    mPA-GFP

    Expression

    Mammalian

    Mutation

    I474N in Calnexin

    Promoter

    CMV

    Available since

    Jan. 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

11. pDx_mScarlet-I3-SYFP2

    Plasmid

    #189763

    Purpose

    Dual expression vector for bacteria and mammalian cells producing mScarlet-I3-SYFP2 fusion protein for FRET analysis

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet-I3-SYFP2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV & Rha

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC18T-mini-Tn7T-Tp

    Plasmid

    #65024

    Purpose

    TpR mini-Tn7 vector, mobilizable

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 6, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. S1m tagged DANCR

    Plasmid

    #138585

    Purpose

    For in vivo tagging

    Depositor

    Yin-Yuan Mo

    Article

    Hu et al Cell Death Differ. 2019 Dec 5. pii: 10.1

    Insert

    DANCR (DANCR Human)
    

    Use

    Lentiviral

    Tags

    S1m tag

    Expression

    Mutation

    Promoter

    MSCV

    Available since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET His6 MBP Asn10 TEV LIC cloning vector (1C)

    Plasmid

    #29654

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    10xAsn, 6xHis, MBP, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-PE7-mutant (Q20A_Y23A_Y24F_F35A)

    Plasmid

    #224123

    Purpose

    Mammalian expression of SpCas9 PE7 mutant prime editor (Q20A_Y23A_Y24F_F35A)

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7-mutant (Q20A_Y23A_Y24F_F35A)
    

    Use

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Q20A_Y23A_Y24F_F35A in La(SSB)1-194

    Promoter

    CMV

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUB_smHA_KDM5B_MS2

    Plasmid

    #81085

    Purpose

    Translation reporter: Encodes KDM5B (1544 aa) fused to smHA (Spaghetti Monster HA: 10 copies of an epitope tag HA).

    Depositor

    Tim Stasevich

    Article

    Morisaki et al Science. 2016 Jun 17;352(6292):1425-9. d

    Insert

    smHA_KDM5B_24xMS2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGW-TurboID-GOI

    Plasmid

    #209380

    Purpose

    Gateway compatible plasmid for TurboID-based proximity labelling experiment in planta

    Depositor

    Rosa Lozano-Durán

    Article

    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    TurboID gene sequence C249A

    Promoter

    Available since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGP-AAV-syn-FLEX-Volton2-ST-WPRE

    Plasmid

    #172907

    Purpose

    AAV-mediated expression of voltage sensor under the Syn promoter, Cre-dependent expression; soma localization targeting sequence

    Depositor

    GENIE Project

    Article

    Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

    Has Service

    AAV1

    Insert

    Voltron2-ST
    

    Use

    AAV and Cre/Lox

    Tags

    soma localization targeting sequence

    Expression

    Mammalian

    Mutation

    A122D

    Promoter

    hSynapsin1

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. Ga13_2-mTq2

    Plasmid

    #112933

    Purpose

    Human Galpha13 tagged with mTurquoise, inserted at residue 128

    Depositor

    Dorus Gadella

    Article

    Mastop et al PLoS One. 2018 Mar 5;13(3):e0193705. doi

    Insert

    Galpha13
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Gal4BD-CRY2(535)

    Plasmid

    #75369

    Purpose

    Yeast two-hybrid expression vector, expresses Gal4BD fused to Arabidopsis CRY2 (residues 1-535)

    Depositor

    Chandra Tucker

    Article

    Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

    Insert

    CRY2 (CRY2 Mustard Weed)
    

    Use

    Tags

    Gal4BD

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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