162,325 results

1. pHD-DsRed

   Plasmid

   #51434

   Purpose

   Vector for generating dsDNA donors for homology-directed repair. Contains the visible marker 3xP3-DsRed.

   Depositor

   Kate O'Connor-Giles

   Article

   Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1

   Insert

   LoxP-3xP3-DsRed-LoxP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   3xP3

   Available since

   Feb. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSXneo 135(T2AG3)

   Plasmid

   #12402

   Depositor

   Titia de Lange

   Article

   Hanish et al Proc Natl Acad Sci U S A. 1994 Sep 13. 9

   Insert

   135(T2AG3)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TXNRD1

   Plasmid

   #38863

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   TXNRD1 (Txnrd1 Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-GFP-MT2A

   Plasmid

   #11613

   Depositor

   Steven Johnson

   Insert

   metallothionein 2A (MT2A Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUltra-hot

   Plasmid

   #24130

   Purpose

   3rd generation Lentiviral vector for bi-cistronic expression of mCherry and the gene of interest

   Depositor

   Malcolm Moore

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 26, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEBG‐AMPK
α1(1‐312)

   Plasmid

   #27632

   Depositor

   Reuben Shaw

   Article

   Egan et al Science. 2011 Jan 28;331(6016):456-61. d

   Insert

   AMPK
α1(1‐312) (Prkaa1 Rat)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   Constitutively
 active
 by 
truncation 
after 
am…

   Promoter

   Available since

   May 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. PL752-EN1-LA-p2a-mTagBFP2nls-3xHAtag-hGHpA-PGK-puroR-EN1-RA

   Plasmid

   #191691

   Purpose

   For knocking p2a-mTagBFP2nls-3xHAtag into EN1 locus

   Depositor

   Yuejun Chen

   Article

   Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

   Insert

   EN1 homology arms and p2a-mTagBFP2nls-3xHAtag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDest-566

   Plasmid

   #11517

   Depositor

   Dominic Esposito

   Type

   Empty backbone

   Use

   Gateway destination vector

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB-TRE-OKSM

   Plasmid

   #204498

   Purpose

   Expresses Oct4, Klf4, Sox2 and c-Myc by tet-on promoter in mammalian cells

   Depositor

   Wataru Fujii

   Article

   Matsuya et al Sci Rep. 2024 Feb 8;14(1):3204. doi: 10.

   Inserts

   OCT4 (POU5F1 Human)
   KLF4 (KLF4 Human)
   SOX2 (SOX2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. acGFP Peroxisome visual barcode

    Plasmid

    #158674

    Purpose

    tagging clonal populations with Peroxisomal acGFP fluorescent protein. Adding Peroxisome localization signal to acGFP WC

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Insert

    acGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. TC1332

    Plasmid

    #164874

    Purpose

    CURE-N: C to U RNA Editing, with high efficiency and low off-target effects

    Depositor

    Tian Chi

    Article

    Huang et al EMBO J. 2020 Nov 16;39(22):e104741. doi:

    Insert

    hAPOBEC3A fused to PspCas13b (APOBEC3A )
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    human apobec Y132D

    Promoter

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. CL20_mEGFP_ETV6_RUNX1

    Plasmid

    #205818

    Purpose

    Express mEGFP-tagged fusion protein, ETV6_RUNX1 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    ETV6_RUNX1 (ETV6, RUNX1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. hSyn-somBiPOLES-mCerulean

    Plasmid

    #154945

    Purpose

    Optogenetic tool for blue-light inhibition and red-light excitation of neurons

    Depositor

    Simon Wiegert

    Article

    Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

    Insert

    somBiPOLES
    

    Use

    AAV

    Tags

    soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin

    Available since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. p1177-X1-8

    Plasmid

    #38150

    Depositor

    David Waugh

    Article

    Austin et al Protein Expr Purif. 2011 May;77(1):53-61

    Insert

    Carboxypeptidase A
    

    Use

    Tags

    HHHHHHRR

    Expression

    Insect

    Mutation

    Promoter

    polyhedron

    Available since

    Oct. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcdna3.1 hciap1

    Plasmid

    #8311

    Depositor

    Jon Ashwell

    Article

    Li et al Nature 2002 Mar 21;416(6878):345-7.

    Insert

    cIAP1 (BIRC2 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. TFORF2460

    Plasmid

    #142090

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    IRF7 (IRF7 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKOV

    Plasmid

    #25769

    Depositor

    George Church

    Article

    Link et al J Bacteriol. 1997 Oct . 179(20):6228-37.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 3, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. AICSDP-19:LAMP1-mEGFP

    Plasmid

    #101782

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human LAMP1

    Depositor

    Allen Institute for Cell Science

    Insert

    LAMP1 Homology Arms with linker-mEGFP (LAMP1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pQCXIP-GFP-RPA70

    Plasmid

    #164231

    Purpose

    retroviral plasmid for GFP-RPA70

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    RPA70 (RPA1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. SpCas9 TadDE

    Plasmid

    #193837

    Purpose

    Express TadDE (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadA-DualEditor-Cas9D10A-2xUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge