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pVCERC-5 Pvan-dcas9 (Sth3)

Plasmid

#133319

Purpose

expresses dcas9 from Streptococcus thermophilus #3; induced with vanillate; for homologous recombination at the vanillate locus in Caulobacter crescentus; tetracycline resistance

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

dcas9 (Streptococcus thermophilus #3)

Use

Unspecified

Promoter

vanillate

Available Since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTE5400_pMBP_CDS dropout vector

Plasmid

#221189

Purpose

Expression vector for N-terminally His-tagged maltose binding protein (10xHis-MBP) fused to a protein of interest

Depositor

Tobias Erb

Article

Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0

Type

Empty backbone

Tags

10x His-tag + E. coli maltose binding protein (MB…

Expression

Bacterial

Promoter

T7 promoter

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCsC_spacer

Plasmid

#136077

Purpose

Use when making odd level constructs (L3,...) if pCsC position (position 3) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Even spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCsE_spacer

Plasmid

#136078

Purpose

Use when making odd level constructs (L3,...) if pCsE position (position 4) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Even spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCsB

Plasmid

#136068

Purpose

Acceptor plasmid for even level position 2, lacZ blue-white screening

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Synthetic Biology

Expression

Plant

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCsC

Plasmid

#136069

Purpose

Acceptor plasmid for even level position 3, lacZ blue-white screening

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Synthetic Biology

Expression

Plant

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCsE

Plasmid

#136070

Purpose

Acceptor plasmid for even level position 4, lacZ blue-white screening

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Synthetic Biology

Expression

Plant

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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mVenus-RapR-PTPPest

Plasmid

#188657

Purpose

Rapamycin regulated PTPPest with mVenus tag

Depositor

Andrei Karginov

Article

Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

Protein Tyrosine Phosphatase Pest (PTPN12 )

Tags

mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 Orf9b

Plasmid

#158378

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 Orf9b

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pRS-puro shNEDD4L #2

Plasmid

#27017

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

shRNA NEDD4L #2 (NEDD4L Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only
pGAN147

Plasmid

#129202

Purpose

yeast transcriptional reporter of aggregating proteins (yTRAP) to detect protein aggregation, Gateway backbone

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Promoter

SUP35

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-N3-zz-p15_O

Plasmid

#147110

Purpose

Mammalian Expression of zz-p15

Depositor

Elisa Izaurralde

Article

Teplova et al Nat Struct Mol Biol. 2011 Aug 7;18(9):99

Insert

zz-p15 (NXT1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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mEm-CoilE-Actin

Plasmid

#181993

Purpose

CoilE genetically encoded tag on mEmerald-Actin, to be used with CoilR labeled with a reporter

Depositor

Kimberly Beatty

Article

Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

Insert

mEm-CoilE-Actin

Tags

mEmerald

Expression

Mammalian

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pGL3-VEGFR2-570

Plasmid

#21309

Depositor

Donald Ingber

Article

Mammoto et al Nature. 2009 Feb 26. 457(7233):1103-8.

Insert

VEGFR2 promoter (KDR Human)

Use

Luciferase

Available Since

May 29, 2009

Availability

Academic Institutions and Nonprofits only
TC513

Plasmid

#122616

Purpose

Punc-4c::SV40 NLS::rtetR::Zip(+)::SL2::mCherry

Depositor

Tian Chi

Article

Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

Insert

SV40 NLS::rtetR::Zip(+)::SL2::mCherry

Expression

Worm

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pHL 246

Plasmid

#53014

Purpose

AmpR + fimS (off orientation)::gfp::Asp terminator + CamR + ColE1

Depositor

Han Lim

Article

Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

Insert

GFP

Use

Synthetic Biology

Tags

fimS (off orientation)

Expression

Bacterial

Available Since

May 14, 2014

Availability

Academic Institutions and Nonprofits only
pDEST_6xUAS:miRFP670-cetn4

Plasmid

#192357

Purpose

zfCETN4 tagged with miRFP670, expressed under the UAS promoter

Depositor

Darren Gilmour

Article

Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

Insert

CETN4 (cetn4 Zebrafish)

Tags

miRFP670

Expression

Mammalian

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-ChETA-mRuby2-ST

Plasmid

#109126

Purpose

Cation channelrhodopsin ChETA fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

ChETA-mRuby2-ST

Expression

Mammalian

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-104

Plasmid

#79842

Purpose

Expresses NLS-PpsR2-VP16 under CMV promoter

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

Insert

NLS-PpsR2-VP16

Tags

Nuclear localization signal of nuclear cap-bindin…

Expression

Mammalian

Mutation

RpPpsR2 cysteine 439 changed to serine

Promoter

CMV

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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BII-Sh-PnGW

Plasmid

#133359

Purpose

Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

GFP-NLS

Tags

HA-tagged GFP

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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