We narrowed to 14,256 results for: TIM

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pcDNA3.1-deltaA1-VWF

Plasmid

#124823

Purpose

Expresses multimeric human VWF protein without A1 domain. This construct has no tag.

Depositor

Sriram Neelamegham

Article

Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

Insert

VWF without A1 domain (VWF Human)

Expression

Mammalian

Mutation

Deleted A1 domain

Promoter

CMV

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pLN193 (SSX1p-[mK-Ex1]-[miR1-Mv3 intron]-[mK-Ex2]-BS(Pe))

Plasmid

#105210

Purpose

Module 1 - SSX1 promoter drives self-inhibiting mKate2 expression (Version 3 intron - see PMID: 29056342 for detailed information)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

SSX1p-[mK-Ex1]-[miR1-Mv3 intron]-[mK-Ex2]-BS(Pe)

Use

Synthetic Biology

Expression

Mammalian

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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pNHT7::RPL15::hGluc

Plasmid

#186768

Purpose

An mRNA production vector encoding human codon-optimized gaussia luciferase (hGluc) with RPL15 5' and 3' UTRs.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Gaussia luciferase

Promoter

T7

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pVRa16_3622

Plasmid

#49670

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF16_3622

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available Since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pSG5-ERRbeta-delta10

Plasmid

#52187

Purpose

Expresses human ERRbeta-delta10 splice variant in mammalian cells

Depositor

Rebecca Riggins

Article

Heckler et al Cell Cycle. 2014 Dec 12:0.

Insert

Estrogen-related receptor beta (ESRRB Human)

Expression

Mammalian

Mutation

wild type sequence; codon optimized to exclude Ec…

Promoter

SV40

Available Since

April 24, 2014

Availability

Academic Institutions and Nonprofits only
pCXLE-dCas9VPP300-T2A-GFP-shP53

Plasmid

#102896

Purpose

EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-P300core followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

dCas9VPP300-T2A-GFP-shP53

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Promoter

CAG

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pLN520 (SSX1p-[mK-Ex1]-[miR1-Mv1 intron]-[mK-Ex2]-BS(Pe))

Plasmid

#105207

Purpose

Module 1 - SSX1 promoter drives self-inhibiting mKate2 expression (Version 1 intron - see PMID: 29056342 for detailed information)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

SSX1p-[mK-Ex1]-[miR1-Mv1 intron]-[mK-Ex2]-BS(Pe)

Use

Synthetic Biology

Expression

Mammalian

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pC1300_pUB10_pco-nCASphi_E9t_V2_pUB10_E9t_ribozyme_AtPDS3_gRNA10

Plasmid

#197981

Purpose

T-DNA binary vector to express pco-nCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by UBQ10 gene promoter, flanked by ribozymes.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Inserts

pco-nCasphi-2
AtPDS3 gRNA10

Use

CRISPR

Expression

Plant

Promoter

pUBQ10

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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Sox17(2A-phiC31o)

Plasmid

#140762

Purpose

Targeting vector to Sox17 gene locus for induction of P2A-fused phiC31o, with PGK-EM7-puroR drug resistant cassette flanked by FRT(F5) sequence.

Depositor

Hideyuki Okano

Article

Serizawa et al Development. 2019 Nov 4;146(21). pii: de

Insert

phiC31o

Use

Mouse Targeting

Promoter

Endogenous Sox17

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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GB1-PRD(800-868)-strep

Plasmid

#180023

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(800-868)-Strep.

Depositor

Lalit Deshmukh

Article

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

GB1-His6-TEV-ALIX(800-868)-Strep (PDCD6IP Human)

Tags

GB1-His6-TEV and Strep tag (WSHPQFEK)

Expression

Bacterial

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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shNg Lenti FHRC3J1UGW

Plasmid

#92233

Purpose

lentiviral expression of EGFP and Ng shRNA

Depositor

Weifeng Xu

Article

Hwang et al Biol Psychiatry. 2021 Feb 1;89(3):256-26

Insert

shRNA targeting Ng

Use

Lentiviral

Expression

Mammalian

Promoter

H1

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pNHT7::RPL15::sNluc2

Plasmid

#186763

Purpose

An improved mRNA product vector encoding a codon-optimized nanoluciferase (sNluc2) with RPL15 5' and 3' UTRs.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Promoter

T7

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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(B) HYG marker(R) (SBE264)

Plasmid

#195013

Purpose

hygromycin (HYG) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tGPD

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

pXYL+HYG+tGPD

Expression

Bacterial

Promoter

pXYL

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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(B) BLE marker(R) (SBE262)

Plasmid

#195012

Purpose

bleomycin (BLE) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tGPD

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

pXYL+BLE+tGPD

Expression

Bacterial

Promoter

pXYL

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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ET1-human LRRC33

Plasmid

#111603

Purpose

Express human LRRC33 in 293T cells

Depositor

Timothy Springer

Article

Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

Insert

human LRRC33 (NRROS Human)

Tags

PreScission/TEV/ProteinC/6xHIS/SBP/

Expression

Mammalian

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs_SFXN1

Plasmid

#110635

Purpose

Retroviral expression of SFXN1 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Science. 2018 Nov 16;362(6416). pii: 362

Insert

SFXN1 (SFXN1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

codon-optimized cDNA (no amino acid mutations)

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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R4pGWB601_UBQ10p-BirA(R118G)-YFP-NLS

Plasmid

#127365

Purpose

Binary vector for expressing nuclear BirA* (R118G)-YFP under the UBQ10 promoter in plants

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

BirA* (R118G mutant)

Tags

GS linker, NLS, V5, and YFP

Expression

Plant

Mutation

R118G

Promoter

Arabidopsis UBQ10 promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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CoV2-N-D377Y

Plasmid

#177957

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (D377Y). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (D377Y) (N )

Expression

Mammalian

Mutation

D377Y

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLN189 (SSX1p-[mK-Ex1]-[miR1-Mv2 intron]-[mK-Ex2]-BS(Pe))

Plasmid

#105209

Purpose

Module 1 - SSX1 promoter drives self-inhibiting mKate2 expression (Version 2 intron - see PMID: 29056342 for detailed information)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

SSX1p-[mK-Ex1]-[miR1-Mv2 intron]-[mK-Ex2]-BS(Pe)

Use

Synthetic Biology

Expression

Mammalian

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti_dCas9-2xAM_hIRF-1

Plasmid

#92221

Purpose

Lentiviral plasmid expressing dCas9-2xAM and gRNA for human IRF-1 promoter

Depositor

Hodaka Fujii

Article

Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

Inserts

Sp-dCas9-2xAM tag
gRNA targeting human IRF-1 promoter

Use

CRISPR and Lentiviral

Tags

2xAM tag

Expression

Mammalian

Mutation

human codon-optimized, D10A + H840A

Promoter

CBh and U6

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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