We narrowed to 36,691 results for: KAN

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pRSFDuet-1_VNp-Uricase

Plasmid

#182401

Purpose

Bacterial expression of Vesicle Nucleating peptide-uricase fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-uricase

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HsUpf1-siRNAres_P

Plasmid

#147196

Purpose

Mammalian Expression of HsUpf1-siRNAres

Depositor

Elisa Izaurralde

Insert

HsUpf1-siRNAres (UPF1 Human)

Expression

Mammalian

Mutation

5 non silent mutations A69S, E167G, K282R, A442G…

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-Rab9aQ66L

Plasmid

#169072

Purpose

Express human constitutively active form of Rab9a(Q66L) in mammalian cells

Depositor

Gia Voeltz

Article

Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

Insert

Rab9a (RAB9A Human)

Tags

acGFP1

Expression

Mammalian

Mutation

Q66L

Promoter

CMV

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pXD70LacZ6-LacI-Ptac(LacO)-LacZ

Plasmid

#191621

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), IPTG-inducible promoter-lacZ, engineered from pMAL-c2X

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

lacZ

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pXD71Cas10.0-Pct5.1-crRNA(AarI)

Plasmid

#191633

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(nontargeting spacer), entry plasmid for crRNA cloning

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

CymR

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pDL024_Tub-rb_IgG_LC

Plasmid

#193621

Purpose

α-Tubulin light chain variable region + rabbit light chain constant region + exogenous N-term signal peptide. For co-expression with pDL023 in HEK293 cells.

Depositor

Jennifer DeLuca

Article

DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

Insert

Anti-Tubulin scFv light variable + rabbit constant chain

Tags

signal peptide

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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CapChR1_pmCherry-N1

Plasmid

#188031

Purpose

Expresses CapChR1-mCherry in mammalian cells

Depositor

Peter Hegemann

Article

Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

Insert

CapChR1-mCherry

Tags

mCherry

Expression

Mammalian

Mutation

S63D, L132C, T159C and N258E mutations in CrChR2

Promoter

CMV

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR221-APP751

Plasmid

#193772

Purpose

Entry vector for human APP isoform having 751 amino acid residues

Depositor

Wade Harper

Article

Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

Insert

APP (APP Human)

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCR-U6-gRNA-miniCMV-TdTomato

Plasmid

#192656

Purpose

A plasmid encoding miniCMV sgRNA and miniCMV-promoter-TdTomato

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

TdTomato

Expression

Mammalian

Promoter

U6, miniCMV

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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4xmts-G-Ca-FLITS

Plasmid

#191462

Purpose

Genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change

Depositor

Dorus Gadella

Article

van der Linden et al eLife 14:RP105086; doi: https://doi.org/

Insert

G-Ca-FLITS

Tags

4xmts

Expression

Mammalian

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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Tubulin-mhYFP

Plasmid

#186525

Purpose

Localization of monomeric hyperfolder YFP fluorescent protein to tubulin

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Monomeric hyperfolder YFP

Tags

Tubulin

Expression

Mammalian

Mutation

hfYFP-S147P/L195M/V206K

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pSLS.436

Plasmid

#184953

Purpose

Express -Eco1 RT and ncRNA

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1: ncRNA(wt) and RT

Expression

Bacterial

Promoter

T7

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsYTHDF3_AB

Plasmid

#148313

Purpose

Mammalian Expression of HsYTHDF3

Depositor

Elisa Izaurralde

Insert

HsYTHDF3 (YTHDF3 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pBTK1009 (pSL9)

Plasmid

#191004

Purpose

Expresses E2 Crimson on a broad host range pBBR1 origin plasmid

Depositor

Jeffrey Barrick

Article

Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

Insert

E2-Crimson

Use

Synthetic Biology

Expression

Bacterial

Promoter

CP25

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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mcherry-p62 delta PB1 silent

Plasmid

#187985

Purpose

human p62 lacking PB1 domain (=aa1-102) with a silent mutation to get resistance for sip62 #5 (Dharmacon). Internal Reference: SMC552

Depositor

Sascha Martens

Article

Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

Insert

p62 (NUP62 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Lacking PB1 domain (aa1-102) with a silent mutati…

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pET28-MKIEE-NLS-RfxCas13d-NLS-HA-6His

Plasmid

#171587

Purpose

Bacterial expression of NLS-RfxCas13d-NLS 6His

Depositor

Neville Sanjana

Article

Mendez-Mancilla et al Cell Chem Biol. 2022 Feb 17;29(2):321-32

Insert

MKIEE-NLS-RfxCas13d-NLS-HA-6His

Tags

HA-6HIS and MKIEE

Expression

Bacterial

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pTub-alpha1-EB3-NeonGreen-T2A-iLifeact-mCherry

Plasmid

#175261

Purpose

labelling of microtubule plus end and F-actin structures

Depositor

Frank Bradke, Sebastián Dupraz

Article

Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

Insert

EB3-NeonGreen-T2A-iLifeact-mCherry (MAPRE3 Human)

Tags

mCherry (on iLifeact) and mNeonGreen (on EB3)

Expression

Mammalian

Mutation

None (wt)

Promoter

pTub-alpha1

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pSLS.405

Plasmid

#187203

Purpose

Expresses wild-type retron Eco1 ncRNA + Eco1 RT, and Cas1 + Cas2

Depositor

Seth Shipman

Article

Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

Inserts

Retron Eco1 ncRNA (wildtype)
Cas1 + Cas2

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7/Lac

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-SRGAP1

Plasmid

#187269

Purpose

Expression of SRGAP1-EGFP

Depositor

Alpha Yap

Article

Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

Insert

SRGAP1 (SRGAP1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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Bxb1_miRFP703

Plasmid

#183759

Purpose

Bxb1-specific donor plasmid for intergrating a transcription unit for expression of membrane-localised miRFP703

Depositor

Richard Davis

Article

Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

Insert

miRFP703

Tags

Lck lipidation signal

Expression

Mammalian

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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