We narrowed to 18,674 results for: Met;
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pETDUET-GST AO7REwt
Plasmid#213935PurposeGST tagged AO7 encoding aa 126-258 for expression in bacteriaDepositorInsertAO7 aa 126-258 (RNF25 Human)
TagsGSTExpressionBacterialMutationAO7 cloned into the first multiple cloning site o…PromoterT7 promoter-1Available SinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
mVenus-IQ6 R6A
Plasmid#198202PurposeFRET acceptor: Mutated IQ6 domain of Moysin Va tagged with N-terminal mVenus (lower binding affinity)DepositorAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-TKFC in pLJM60
Plasmid#154206PurposeLentiviral vector for stable expression of TKFCDepositorAvailable SinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-STChRger2-TS-EYFP
Plasmid#129393PurposeSoma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.DepositorInsertsoma targeted ChRger2
UseAAVTagsKv2.1-TS-EYFPExpressionMammalianPromoterCaMKIIaAvailable SinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
U6-CjCas9-MS2gRNA-Backbone
Plasmid#210711PurposeThis plasmid express MS2 loop containing Cj Cas9 gRNADepositorInsertMS2 loop containing Cj Cas9 gRNA
UseCRISPRExpressionMammalianPromoterU6Available SinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ITR-EcYtR-mCherry
Plasmid#217362PurposeExpresses E. coli tyrosine tRNA and a wild-type mCherry reporter; can be packaged into AAVDepositorInsertE. coli tyrosine tRNA
UseAAVExpressionMammalianPromoterU6Available SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
KIF5C(1-560)-2xmCherry-EF(C)
Plasmid#61664PurposeTandem mCherry-labeled, truncated kinesin-1 fused to EF Hand linkerDepositorInsertKIF5C(1-560)-2xmCherry-EF(C) (Kif5c Rat)
ExpressionMammalianAvailable SinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-YFP-DEPTOR
Plasmid#73390PurposeExpresses HA and YFP tagged DEPTORDepositorInsertDEP Domain Containing MTOR-Interacting Protein (DEPTOR Human)
TagsHA and YFPExpressionMammalianPromoterCMVAvailable SinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-AcKRS-CloDF
Plasmid#127415PurposeMutant of mmPylRS designed for incorporation of non-canonical amino acids (acyl-lysine derivatives) in to M13 bacteriophageDepositorInsertPyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
ExpressionBacterialPromoteraraBADAvailable SinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-surrogate-reporter
Plasmid#65624PurposeFluorescent reporter to monitor the activty of Bxb1; If Bxb1 and attB donor plasmid containing GFP is present in the cell, recombination of the donor into the reporter leads to expression of GFP.DepositorInsertattP
UseBxb1ExpressionMammalianAvailable SinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RIPK3[V458A][Q459A][V460A][G461A]-FLAG
Plasmid#199328Purposemutated RHIM motive (interaction inhibited)DepositorInsertReceptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)
TagsFLAGExpressionMammalianMutationchanged V458, Q459,V460 and G461 to alanineAvailable SinceJune 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET23a-His-hRanGAP1tail
Plasmid#53139PurposeE.coli expression of Cterminal domain of human RanGAP1 with Nterminal His-tagDepositorInsertRanGAP1 (RANGAP1 Human)
TagsHisExpressionBacterialMutationC-terminal fragment (amino acids 398-587)PromoterT7Available SinceMay 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CBX1mut-PA-mCherry
Plasmid#138251PurposeTriple-mutant CBX1 chromodomain (residues 20-73) tagged with photoactivatable mCherry (PAmCherry). Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1.DepositorInsertCBX1mut-PAmCherry
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
CIBN-GFP-TMD
Plasmid#79571Purposeexpression of CIBN domain fused to GFP tagged mouse Syntaxin1 TMD domain (transmembrane domain and polybasic region)DepositorInsertsyntaxin 1A (Stx1a Mouse)
TagsCIBN and EGFPExpressionMammalianMutationaa 249_288 (C-terminal region (polybasic stretch …PromoterCMVAvailable SinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn_SpikeyGi2_kv2.1
Plasmid#195313PurposeGenetically-encoded voltage indicator SpikeyGi2, soma and AIS targeted, in AAV vector under the neuronal-specific promoter hSynDepositorInsertSpikeyGi2
UseAAVPromoterhSynAvailable SinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCarCBADE_ProB(I69E)A
Plasmid#117756PurposeProB(I69E)A and codon optimized CarCBADE enzymes (in that order) in pBbaA5k backboneDepositorInsertGlutamate kinase (ProA), mutant pyrroline-5-carboxylate synthase (ProBI69E) from E. coli and codon optimised CarCBADE operon
ExpressionBacterialPromoterPLac(UV5)Available SinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV hSyn hPGK1-mRuby
Plasmid#220911PurposeNeuronal specific expression of the fusion construct PGK1-mRubyDepositorAvailable SinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSB97 - pL2_pVS1_2x35S::fLUC-I::tNOS
Plasmid#123189Purposebinary plant vector for transient fLUC (with intron) expressionDepositorInsert2x35S::fLUC-I::tNOS
UseLuciferaseExpressionPlantAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
CRY2-OCRL(D523G)
Plasmid#79567Purposeexpression of CRY2PHR fused to phosphatase dead mutant of OCRLDepositorInsertOCRL (OCRL Human)
TagsCRY2 (photolyase homology region domain of Arabid…ExpressionMammalianMutationaa 234_539 and phosphatase mutation, D523GPromoterCMVAvailable SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only
