We narrowed to 14,584 results for: pET

1. pET21-pelB-GST-3

   Plasmid

   #228985

   Purpose

   For bacterial expression of anti-GST nanobody GST-3, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   GST-3
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET21-pelB-LaMIgG-14

   Plasmid

   #228994

   Purpose

   For bacterial expression of anti-mouse IgG nanobody LaMIgG-14, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaMIgG-14
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET21-pelB-LaG94-2

   Plasmid

   #228962

   Purpose

   For bacterial expression of anti-GFP nanobody LaG94-2, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaG94-2
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET21-pelB-LaG94-5

   Plasmid

   #228965

   Purpose

   For bacterial expression of anti-GFP nanobody LaG94-5, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaG94-5
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET21-pelB-LaG94-1

   Plasmid

   #228961

   Purpose

   For bacterial expression of anti-GFP nanobody LaG94-1, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaG94-1
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET21-pelB-LaMIgG-9

   Plasmid

   #228993

   Purpose

   For bacterial expression of anti-mouse IgG nanobody LaMIgG-9, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaMIgG-9
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET21-pelB-LaTdT-1

   Plasmid

   #228974

   Purpose

   For bacterial expression of anti-tdTomato nanobody LaTdT-1, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaTdT-1
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET21-pelB-LaRIgG-7

   Plasmid

   #228997

   Purpose

   For bacterial expression of anti-rabbit IgG nanobody LaRIgG-7, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaRIgG-7
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET21-pelB-LaTdT-44

   Plasmid

   #228981

   Purpose

   For bacterial expression of anti-tdTomato nanobody LaTdT-44, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaTdT-44
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET21-pelB-LaTdT-45

    Plasmid

    #228982

    Purpose

    For bacterial expression of anti-tdTomato nanobody LaTdT-45, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaTdT-45
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET21-pelB-LaMIgG-8

    Plasmid

    #228992

    Purpose

    For bacterial expression of anti-mouse IgG nanobody LaMIgG-8, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaMIgG-8
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET21-pelB-LaTdT-10

    Plasmid

    #228978

    Purpose

    For bacterial expression of anti-tdTomato nanobody LaTdT-10, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaTdT-10
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET21-pelB-LaRIgG-1

    Plasmid

    #228995

    Purpose

    For bacterial expression of anti-rabbit IgG nanobody LaRIgG-1, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaRIgG-1
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET21-pelB-GST-8

    Plasmid

    #228990

    Purpose

    For bacterial expression of anti-GST nanobody GST-8, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    GST-8
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET21-pelB-LaG94-4

    Plasmid

    #228964

    Purpose

    For bacterial expression of anti-GFP nanobody LaG94-4, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaG94-4
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET21-pelB-LaMIgG-5

    Plasmid

    #228991

    Purpose

    For bacterial expression of anti-mouse IgG nanobody LaMIgG-5, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaMIgG-5
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET21-pelB-GST-5

    Plasmid

    #228987

    Purpose

    For bacterial expression of anti-GST nanobody GST-5, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    GST-5
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET21-pelB-GST-6

    Plasmid

    #228988

    Purpose

    For bacterial expression of anti-GST nanobody GST-6, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    GST-6
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET21-pelB-LaTdT-8

    Plasmid

    #228976

    Purpose

    For bacterial expression of anti-tdTomato nanobody LaTdT-8, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaTdT-8
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET21-pelB-LaTdT-9

    Plasmid

    #228977

    Purpose

    For bacterial expression of anti-tdTomato nanobody LaTdT-9, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaTdT-9
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET21-pelB-LaTdT-39

    Plasmid

    #228980

    Purpose

    For bacterial expression of anti-tdTomato nanobody LaTdT-39, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaTdT-39
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET28b-SUMO-SeKaiA

    Plasmid

    #229218

    Purpose

    For expression of S. elongatus KaiA protein

    Depositor

    Andy LiWang

    Insert

    kaiA (kaiA S. elongatus)
    

    Use

    Tags

    FLAG and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET-MhCINS-6xHis

    Plasmid

    #194304

    Purpose

    Bacterial expression of MhCINS protein with a C terminal His-tag and transit peptide removed at N terminal

    Depositor

    Richard Saunders

    Article

    Liu et al J Ecology 2024; 00, 1–20. doi: 10.1111/1

    Insert

    MhCINS
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET-6xHis-GFP(-12)

    Plasmid

    #219420

    Purpose

    Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

    Depositor

    Allie Obermeyer

    Article

    Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

    Insert

    GFP(-12)
    

    Use

    Tags

    MGHHHHHGG

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET20b-mPTER-R204A

    Plasmid

    #222196

    Purpose

    Bacterial expression of mouse PTER-R204A protein

    Depositor

    Jonathan Long

    Article

    Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

    Insert

    PTER (Pter Mouse)
    

    Use

    Tags

    6xHis and Strep

    Expression

    Bacterial

    Mutation

    R204A

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pETM6-T7-VHH-GFPminimizer

    Plasmid

    #202488

    Purpose

    Expresses the nanobody GFP minimizer with T7 induction

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    nanobody GFP minimizer
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pETM6-T7-VHH-C9BoNT-A

    Plasmid

    #202490

    Purpose

    Expresses the nanobody C9 BoNT/A with T7 induction

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    nanobody C9 BoNT/A
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pETM6-Amp-T7-GFPuv

    Plasmid

    #202463

    Purpose

    Expresses GFPuv with T7 induction

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    GFPuv
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET-Cas9-FCPF

    Plasmid

    #220132

    Purpose

    Expresses Cas9-FCPF in E.coli

    Depositor

    Xinlai Cheng

    Article

    Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

    Insert

    Cas9-FCPF (cas9 S. pyogenes)
    

    Use

    Tags

    His and NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET28-acatenin.22-906

    Plasmid

    #186460

    Purpose

    Expresses human alpha-catenin (residues 22-906) with an N-terminal His tag and a PRescission Protease cleavage site

    Depositor

    Tina Izard

    Article

    Rangarajan et al Commun Biol. 2023 Mar 16;6(1):276. doi:

    Insert

    CTNNA1 (CTNNA1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET28a-CsHPAD_beta-gamma

    Plasmid

    #226981

    Purpose

    Expressing Clostridium scatologenes hydroxyphenylacetate decarboxylase (HPAD) beta/gamma subunits in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    CsHPAD_beta-gamma
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET28a-OuIAD(W392A)

    Plasmid

    #226980

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) W392A variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(W392A)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    W392A

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET28a-OuIAD(W392F)

    Plasmid

    #226979

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) W392F variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(W392F)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    W392F

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET28a-OuIAD(F401A)

    Plasmid

    #226978

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) F401A variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(F401A)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    F401A

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET28a-OuIAD(L616E)

    Plasmid

    #226977

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) L616E variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(L616E)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    L616E

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET28a-OuIAD(R226K)

    Plasmid

    #226976

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) R226K variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(R226K)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    R226K

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET28a-OuIAD(R226M)

    Plasmid

    #226975

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) R226M variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(R226M)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    R226M

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET28a-OuIAD(R226E)

    Plasmid

    #226974

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) R226E variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(R226E)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    R226E

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET28a-OuIAD(H514E)

    Plasmid

    #226972

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) H514E variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(H514E)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    H514E

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET28a-OuIAD(H514A)

    Plasmid

    #226971

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) H514A variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(H514A)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    H514A

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge