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We narrowed to 3,141 results for: NAK

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Showing: 2901 - 2950 of 3141 results

  1. pJC2527 - pET28-SKP1(A2P, ∆38-43, ∆71-82)-StrepII-FBXO31(∆1-65)

    Plasmid
    #250252
    Purpose
    Recombinant production of SKP1(A2P, ∆38-43, ∆71-82) and 6His-Smt3-StrepII-FBXO31(∆1-65).
    Depositor
    Jacob Corn
    Article
    Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:
    Inserts
    SKP1 (SKP1 Human)
    FBXO31 (FBXO31 Human)
    Tags
    6xHis-Smt3(SUMO)-StrepII
    Expression
    Bacterial
    Mutation
    A2P, ∆38-43, ∆71-82 and ∆1-65
    Promoter
    T7
    Available Since
    Jan. 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    250252-plasmid-map-sequence-id-498556

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  2. pαH-S-GSAS-2P-N317P

    Plasmid
    #196378
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein with mutation N317P with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987
    Depositor
    Priyamvada Acharya
    Article
    Manrique et al iScience. 2023 Jan 20;26(1):105855. doi:
    Insert
    S-GSAS-2P-N317P (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV 3C cleavage site (before tags) C terminal on …
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available Since
    March 24, 2023
    Availability
    Academic Institutions and Nonprofits only

  20. pSLIK-NFLAG-hEWSR1G511A-CMyc

    Plasmid
    #50734
    Purpose
    Produces lentivirus expressing human EWSR1G511A mutant with FLAG tag at its N-terminus and Myc tag at its C-terminus by co-transfection with psPAX2 and pMD2G
    Depositor
    Zissimos Mourelatos
    Article
    Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91
    Insert
    EWSR1 G511A (EWSR1 Human)
    Use
    Lentiviral
    Tags
    FLAG and Myc
    Mutation
    Changed Gly 511 to Ala
    Promoter
    TRE
    Available Since
    Jan. 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50734-plasmid-map-sequence-id-481096

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  25. pSLIK-NFLAG-hEWSR1P552L-CMyc

    Plasmid
    #50736
    Purpose
    Produces lentivirus expressing human EWSR1P552L mutant with FLAG tag at its N-terminus and Myc tag at its C-terminus by co-transfection with psPAX2 and pMD2G
    Depositor
    Zissimos Mourelatos
    Article
    Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91
    Insert
    EWSR1 P552L (EWSR1 Human)
    Use
    Lentiviral
    Tags
    FLAG and Myc
    Mutation
    Changed Pro 552 to Leu
    Promoter
    TRE
    Available Since
    Jan. 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  30. pJC2747 - pET28-SKP1(A2P, ∆38-43, ∆71-82)-FBXO31(∆1-65, D334N)

    Plasmid
    #250278
    Purpose
    Recombinant production of SKP1(A2P, ∆38-43, ∆71-82) and 6His-Smt3-StrepII-FBXO31(∆1-65, D334N).
    Depositor
    Jacob Corn
    Article
    Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:
    Inserts
    SKP1 (SKP1 Human)
    FBXO31 (FBXO31 Human)
    Tags
    6xHis-Smt3(SUMO)-StrepII
    Expression
    Bacterial
    Mutation
    A2P, ∆38-43, ∆71-82 and ∆1-65, D334N
    Promoter
    T7
    Available Since
    Jan. 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    250278-plasmid-map-sequence-id-498555

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  33. pMT646 human L1 ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro

    Plasmid
    #213026
    Purpose
    Expresses full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence) with ORF2-3xFlag
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    213026-plasmid-map-sequence-id-422069

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  34. pRT006.3 L1 dual-luciferase AI retrotransposition reporter

    Plasmid
    #213031
    Purpose
    Bi-directional luciferase antisense intron (firefly fluc AI) human LINE-1 retrotransposition reporter (ORFeus-Hs sequence) for sleeping beauty integration
    Depositor
    Donna Romero, Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Tet-On Human LINE-1 (ORFeus-Hs) Firefly + Renilla Luciferase Antisense Intron dual retrotransposition marker
    Use
    Luciferase
    Tags
    Firefly luciferase antisense intron (3' UTR)
    Expression
    Mammalian
    Promoter
    pTRE bidirectional tet-on
    Available Since
    July 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    213031-plasmid-map-sequence-id-422025

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  36. pLD564 human L1 ORF2p-3xFlag (L1RP, CMV promoter) in pCEP4 Puro

    Plasmid
    #213030
    Purpose
    Expresses full length human LINE-1 in human cells (native L1RP sequence), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (L1RP Sequence) with 5' UTR and ORF2-3xFlag
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    213030-plasmid-map-sequence-id-422062

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  40. pMT870 human L1 RT- (ORF2p D702Y) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid
    #213027
    Purpose
    Expresses reverse transcriptase dead (RT- ORF2p D702Y) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence, RT- D702Y) with ORF2-3xFlag
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    D702Y (RT- reverse transcriptase catalytic dead)
    Promoter
    CMV
    Available Since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    213027-plasmid-map-sequence-id-452358

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  41. pMT647 human L1 ORF2p-3xFlag only (ORFeus-Hs, CMV promoter, monocistronic construct) in pCEP4 Puro (derivative of pMT646)

    Plasmid
    #213029
    Purpose
    Expresses human LINE-1 ORF2 only in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 ORF2p only (monocistronic, codon optimized ORFeus-Hs sequence) with ORF2-3xFlag
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    213029-plasmid-map-sequence-id-422050

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  42. pMT1093 human L1 EN- (ORF2p E43S, D145N) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid
    #213028
    Purpose
    Expresses endonuclease dead (EN- ORF2p E43S, D145N) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence, EN- E43S D145N) with ORF2-3xFlag
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    E43S D145N double mutant EN- endonuclease catalyt…
    Promoter
    CMV
    Available Since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    213028-plasmid-map-sequence-id-422046

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  48. pPB-C6F/TdTomato

    Plasmid
    #140826
    Purpose
    OCT4, SOX2, KLF4, C-MYC, KLF2, NANOG (C6F) and tdTomato under the control of TRE (Tet-ON)
    Depositor
    Hideyuki Okano
    Article
    Kisa et al Stem Cell Reports. 2017 Dec 12;9(6):1825
    Inserts
    OCT4 (POU5F1 Human)
    SOX2 (SOX2 Human)
    KLF4 (KLF4 Human)
    C-MYC (MYC Human)
    KLF2 (KLF2 Human)
    NANOG (NANOG Human)
    tdTomato
    Expression
    Mammalian
    Mutation
    L104P- please see depositor comments
    Available Since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    140826-plasmid-map-sequence-id-307041

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Showing: 2901 - 2950 of 3141 results