159,557 results

1. mEos4b-CenpA-C-22

   Plasmid

   #62775

   Purpose

   Localization: Nucleus, C1 Cloning Vector, Excitation:505 / 569, Emission:516 / 581

   Depositor

   Michael Davidson, Loren Looger

   Insert

   CenpA (CENPA Human)
   

   Use

   Tags

   mEos4b

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   April 1, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. mCherry-FKBP-SAC1deltaTMD

   Plasmid

   #108132

   Purpose

   Recruitable cytosolic SAC1

   Depositor

   Gerry Hammond

   Article

   Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

   Insert

   mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L(1-521) (SACM1L Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   SACM1L(1-521)

   Promoter

   CMV

   Available since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGL3-NurRE3

   Plasmid

   #208253

   Purpose

   pGL3 promoter luciferase reporter vector containing 3 copies of the NurRE POMC promoter DNA response element (5′-GATCGTGATATTTACCTCCAAATGCCA- 3′) used for luciferase reporter assays in mammalian cells

   Depositor

   Douglas Kojetin

   Article

   Munoz-Tello et al J Med Chem. 2020 Dec 24;63(24):15639-156

   Insert

   3X Nur response element LUC
   

   Use

   Tags

   No

   Expression

   Mammalian

   Mutation

   Promoter

   SV40 early promoter

   Available since

   Feb. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET_sfCherry(1-10)_32aalinker_sfCherry(11)

   Plasmid

   #83030

   Purpose

   Bacterial expression vector for split sfCherry screening

   Depositor

   Bo Huang

   Article

   Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

   Insert

   sfCherry(1-10)_32aalinker_sfCherry(11)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEJS477-pHAGE-TO-Spy dCas9 3XmCherry-SgRNA/Telomere-All-in-one

   Plasmid

   #85717

   Purpose

   Expresses dSpyCas9-3XmCherry and telomere-targeting Spy sgRNA

   Depositor

   Erik Sontheimer

   Article

   Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

   Inserts

   Sp dCas9
   telomere sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   3XmCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TO and U6

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pYHY3

   Plasmid

   #216109

   Purpose

   aTC-inducible expression of secretory BT3630 SP-NanoLuc via P1TDP-A21 promoter-RBS pair in Bacteroides species.

   Depositor

   Shannon Sirk

   Article

   Yeh et al Nat Commun. 2024 Nov 11;15(1):9741. doi:

   Insert

   NanoLuc
   

   Use

   Synthetic Biology; E. coli-bacteroides shuttle ve…

   Tags

   3xFLAG tag and BT3630 signal peptide

   Expression

   Bacterial

   Mutation

   Promoter

   P1TDP-A21

   Available since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDONR221_SLC2A2

   Plasmid

   #132126

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC2A2 (SLC2A2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pcDNA3.2 TDP-43 NLS1 YFP

   Plasmid

   #84912

   Purpose

   Mammalian expresion of TDP-43 NLS1 YFP

   Depositor

   Aaron Gitler

   Article

   Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   K82A, R83A, K84A

   Promoter

   CMV

   Available since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MS2-Snapf

   Plasmid

   #65860

   Purpose

   Expresses RNA-binding protein MS2 with fast SNAP fusion tag

   Depositor

   Aaron Hoskins

   Article

   Carrocci et al Analyst. 2014 Jan 7;139(1):44-7. doi: 10

   Inserts

   MS2
   Fast SNAP
   

   Use

   Tags

   HA and NLS

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Sept. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

10. dCas9-CBP core

    Plasmid

    #179543

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. Pyogenes dCas9 with c-terminal human CBP core (aa 1084-1701) (CREBBP Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pC0042-PguCas13b crRNA backbone

    Plasmid

    #103853

    Purpose

    For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    U6-PguCas13b DR-BbsI-BbsI-polyT
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pWXC077

    Plasmid

    #222960

    Purpose

    C. elegans codon-optimized mScarlet3 with 3 introns cloned into pPD95.75 backbone

    Depositor

    Oliver Hobert

    Article

    Cao et al Genetics. 2024 Oct 7;228(2):iyae126. doi

    Insert

    mScarlet3
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCiPS

    Plasmid

    #60011

    Purpose

    Foamy virus helper plasmid expressing Pol, driven by a CMV promoter. Ampicillin resistant.

    Depositor

    David Russell

    Article

    Trobridge et al Mol Ther. 2002 Sep;6(3):321-8.

    Insert

    Pol
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ELONGIN BC (XX01TCEB1A-c001)

    Plasmid

    #110274

    Purpose

    Elongin B and Elongin C co-expression plasmid for structure determination; Full length Elongin B & amino acids 17-112 of Elogin C

    Depositor

    Nicola Burgess-Brown

    Insert

    Elongin BC (ELOC, ELOB Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Elongin B is full length. Elongin C contains a.a…

    Promoter

    T7

    Available since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3-smURFP-StagRFP-EKAR-EV

    Plasmid

    #173019

    Purpose

    Red/far-red FRET-based ERK activity reporter for multiplexed imaging applications.

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

    Insert

    smURFP-StagRFP-EKAR-EV
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMT-flp

    Plasmid

    #67274

    Purpose

    Conjugally transferable Flp recombinase plasmid for unmarked mutagenesis

    Depositor

    Mark Liles

    Article

    Hossain et al Biotechnol Rep (Amst). 2015 Aug 28;8:24-

    Insert

    tetA, flp, repA101, oriR101
    

    Use

    Flp recombinase plasmid, temperature sensitive

    Tags

    His-SUMO (flp)

    Expression

    Mutation

    Promoter

    tetA promoter of plasmid pACYC184 origin

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-SMVP-Cas9N

    Plasmid

    #80930

    Purpose

    Expresses Cas9N in mammalian cells.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9N
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-PKA-SPARK

    Plasmid

    #106920

    Purpose

    Express PKA-SPARK, an activity reporter of protein kinase A in mammalian cells.

    Depositor

    Xiaokun Shu

    Article

    Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

    Insert

    PKA-SPARK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAJM.677

    Plasmid

    #108530

    Purpose

    AraCAM + AraE + PBAD-YFP Reporter. Ara sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    AraCAM + AraE + PBAD-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    AraC: L133F E165L E169G C280L

    Promoter

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTECH-MmAcK3RS(IPYE)

    Plasmid

    #104071

    Purpose

    Expression plasmid encoding MmAcK3RS(IPYE) and pylT

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Plpp MmAcK3RS(IPYE); PproK pyl
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Contains activating mutations V31I, T56P, H62Y, A…

    Promoter

    Plpp and PproK

    Available since

    Dec. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge