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pAC-crRNA-Cm

Plasmid

#158711

Purpose

Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNA

Depositor

Yi-Cheng Sun

Article

Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

Insert

eforCP (for crRNA cloning)

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

TetO

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B8-TEF(136)

Plasmid

#44380

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-TEF(136) promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-TEF(136)

Available Since

April 17, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pHR_EF1a-->Gss52SR4-LaM4-P2A-NLSIFP

Plasmid

#162229

Purpose

Lentiviral vector for the expression of Gss52SR4-LaM4-P2A-NLSIFP in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

LaG17 GFP nanobody SynNotch tTA

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pFlag-CMV2-WBP2

Plasmid

#27478

Depositor

Marius Sudol

Article

Chen et al J Biol Chem. 1997 Jul 4. 272(27):17070-7

Insert

WW domain binding protein-2 (WBP2 Human)

Tags

Flag

Expression

Mammalian

Available Since

March 15, 2011

Availability

Academic Institutions and Nonprofits only
pVKG1-Puro

Plasmid

#108670

Purpose

Puromycin resistant cassette for knock-in by VIKING method

Depositor

Shigeo Sugano

Article

Sawatsubashi et al Sci Rep. 2018 Jan 12;8(1):593. doi: 10.1

Insert

PURO

Use

CRISPR and Synthetic Biology

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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p221z-dCAS9p-t35s

Plasmid

#118387

Purpose

Entry clone containing dCAS9p and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

Insert

dCAS9p

Use

CRISPR

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pUPD3

Plasmid

#118043

Purpose

GoldenBraid2.0 Universal Part Domesticator #3

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Type

Empty backbone

Use

Synthetic Biology; Domestication of dna parts for…

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pLCS108

Plasmid

#209329

Purpose

Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal mCherry fusion protein.

Depositor

Lucia Strader

Article

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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Sox17 GFP

Plasmid

#23334

Depositor

Sean Morrison

Article

Kim et al Cell. 2007 Aug 10. 130(3):470-83.

Insert

Sex determining region Y-box containing gene 17 (Sox17 Mouse)

Use

Mouse Targeting

Mutation

None

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV-CamKII-DIO-mCherry

Plasmid

#190241

Purpose

Expresses mCherry flanked by DIO

Depositor

Young J Yoon

Article

Nasrallah et al Proc Natl Acad Sci U S A. 2022 Aug 9;119

Insert

mCherry

Use

AAV

Tags

N/A

Promoter

mouse CamKII alpha

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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OgeuIscB ωRNA-v13

Plasmid

#219634

Purpose

human U6-driven expression of OgeuIscB omegaRNA-v13 (ωRNA-v13) scaffold with BsmBI for guide cloning

Depositor

Weixin Tang

Article

Yan et al Nat Chem Biol. 2024 Jul 8. doi: 10.1038/

Insert

OgeuIscB-ωRNA-v13 scaffold with BsmBI for guide cloning

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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Zic4-3505:GFP

Plasmid

#193001

Purpose

Plasmid to generate a transgenic Hydra Zic4 reporter line

Depositor

Brigitte Galliot

Article

Vogg et al bioRxiv 2021.12.22.47383

Insert

Hydra Zic4 promoter

Use

Hydra transgenesis vector

Tags

GFP

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pFBNb-cALFA

Plasmid

#159990

Purpose

For the protein expression with ALFA tag at C-terminus in insect cells

Depositor

Motoyuki Hattori

Article

Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

Type

Empty backbone

Tags

HRV3C protease cleavage site-ALFA tag-8histidine

Expression

Insect

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-Syx1

Plasmid

#35706

Depositor

Arie Horowitz

Article

Liu et al Mol Biol Cell. 2006 Apr;17(4):1880-7. Ep

Insert

Syx1 (Plekhg5 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

R205K

Promoter

CMV

Available Since

June 22, 2012

Availability

Academic Institutions and Nonprofits only
pLRD16

Plasmid

#208268

Purpose

Production of syn-stemar-13-ene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
osCPS4
osKSL8

Expression

Bacterial

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pOGG026

Plasmid

#113992

Purpose

Level 1 golden gate cloning vector for low copy number stable environmental gene expression

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Type

Empty backbone

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2-GFP1-9

Plasmid

#124433

Purpose

Expresses GFP1-9 in zebrafish

Depositor

Xiaokun Shu

Article

Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

Insert

GFP1-9

Use

Zebrafish

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pCAS9-mCherry-TUBB

Plasmid

#66942

Purpose

CRISPaint target selector TUBB

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

gRNA TUBB

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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p413TEF-PKM2

Plasmid

#34608

Depositor

Markus Ralser

Article

Bluemlein et al Oncotarget. 2011 May;2(5):393-400.

Insert

PKM2 (PKM Human)

Expression

Yeast

Promoter

TEF

Available Since

Jan. 11, 2012

Availability

Academic Institutions and Nonprofits only

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ROPY (sYFP2-OP18-mScI-R125I)

Plasmid

#128002

Purpose

Improved redshifted FRET-readout of Stathmin/OP18 interaction with α-tubulin

Depositor

Dorus Gadella

Article

Van Geel et al Sci Rep. 2020 Apr 7;10(1):6034. doi: 10.

Insert

sYFP2-OP18-mScI-R125I

Expression

Mammalian

Mutation

R125I in mScarlet-I

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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