We narrowed to 23,204 results for: ESC

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Str-Ii_SBP-EGFP-Golgin84

Plasmid

#65303

Purpose

synchronize trafficking of Golgin-84 from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Golgin-84 (GOLGA5 Human)

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pLV-UbC-VP64-dCas9-VP64

Plasmid

#232179

Purpose

Lentiviral cassette that expresses 2xVP64-dCas9 fusion in mammalian cells

Depositor

Charles Gersbach

Article

Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

Insert

VP64-dCas9-VP64

Use

Lentiviral

Expression

Mammalian

Mutation

D10A, H840A

Promoter

hUbC

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCDH-CMV-V5-RFP-LATS1

Plasmid

#235689

Purpose

Express RFP tagged LATS1 gene in mammalian cells using the CMV promoter

Depositor

Duojia Pan

Article

Wang et al Cell. 2022 Nov 10;185(23):4376-4393.e18.

Insert

LATS1 (LATS1 Human)

Use

Lentiviral

Tags

V5-RFP

Expression

Mammalian

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.hSyn.iGluSnFr.WPRE.SV40

Plasmid

#98929

Purpose

AAV expression of glutamate sensor from human synapsin promoter. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

Has Service

AAV1, AAV5, and AAV9

Insert

iGluSnFr

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-(n1)StayGold(c4)-P2A-mCherry

Plasmid

#229603

Purpose

Expresses (n1)StayGold(c4) and mCherry in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

Insert

(n1)StayGold(c4)

Use

AAV

Expression

Mammalian

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-DIO eHcKCR1 3.0-oScarlett-KV2.1

Plasmid

#195198

Purpose

all-optical silencing method

Depositor

Karl Deisseroth

Article

Fan et al Cell. 2023 Feb 2;186(3):543-559.e19. doi

Insert

eHcKCR1 3.0-oScarlet-Kv2.1

Use

AAV

Promoter

CaMKIIa

Available Since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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TPC2-mCherry

Plasmid

#135183

Purpose

Expresses TPC2 with C-terminus mCherry tag in mammalian cells

Depositor

Antony Galione

Article

Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

Insert

TPC2 (Tpcn2 Mouse)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pLPC-LaminA

Plasmid

#69059

Purpose

encodes the LaminA-CS protein

Depositor

Gerardo Ferbeyre

Article

Moiseeva et al Cell Cycle. 2015 Aug 3;14(15):2408-21. d

Insert

LaminA-CS (LMNA Human)

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only
LENTICRISPR-TYMS_sgRNA1

Plasmid

#201628

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

TYMS (TYMS Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-CMV-V5-RFP-SLMAP (isoform r)

Plasmid

#235685

Purpose

Express RFP tagged SLMAP gene in mammalian cells using the CMV promoter

Depositor

Duojia Pan

Article

Wang et al Cell. 2022 Nov 10;185(23):4376-4393.e18.

Insert

SLMAP (SLMAP Human)

Use

Lentiviral

Tags

V5-RFP

Expression

Mammalian

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pSP64T acvr1ba*

Plasmid

#185409

Purpose

Constitutively active form of acvr1ba (Nodal receptor). For in vitro transcription (SP6)

Depositor

Nicolas David

Article

Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

Insert

acvr1ba (acvr1ba Zebrafish)

Use

In vitro synthesis of mrna

Expression

Mammalian

Mutation

changed Threonine 206 to Aspartic acid

Available Since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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pGreenFire_blastocidin

Plasmid

#174103

Purpose

pGreenFire MPRA plasmid for cloning

Depositor

Paul Khavari

Article

Guo et al Nat Genet. 2023 Nov;55(11):1876-1891. do

Type

Empty backbone

Use

Lentiviral

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).iGlucoSnFR2.mIRFP670nano3

Plasmid

#244072

Purpose

Cytosolic expression of green glucose sensor with non-responsive mIRFP

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mIRFP670nano3

Use

AAV

Tags

mIRFP670nano3

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLM-mCherry-Klf4

Plasmid

#23243

Depositor

Michel Sadelain

Article

Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

Insert

mCherry_2A_KLF4 (KLF4 synthetic fluorescent protein, Human)

Use

Lentiviral

Available Since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only

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TPC1-mCherry

Plasmid

#135182

Purpose

Expresses TPC1 with C-terminus mCherry tag in mammalian cells

Depositor

Antony Galione

Article

Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

Insert

TPC1 (Tpcn1 Mouse)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ULK2

Plasmid

#97075

Purpose

Expresses ULK2 in mammalian cells

Depositor

Pasquale Vito

Article

Scudiero et al Cell Death Dis. 2017 Feb 23;8(2):e2627.

Insert

ULK2 (ULK2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-GTSE1

Plasmid

#224719

Purpose

For expression of HA-tagged GTSE1 protein

Depositor

Michele Pagano

Article

Garcia-Vazquez et al bioRxiv [Preprint]. 2025 Jan 23:2024.06.

Insert

GTSE1 (GTSE1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pMito-LSSmKate1-N1

Plasmid

#31878

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

human cytochrome C oxidase subunit VIII (COX8A Human)

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

Available Since

Sept. 9, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV-Myc CDC4 WT*

Plasmid

#16652

Depositor

Bert Vogelstein

Article

Rajagopalan et al Nature. 2004 Mar 4. 428(6978):77-81.

Insert

CDC4 (FBXW7 Human)

Tags

myc

Expression

Mammalian

Mutation

*Note that this insert is only WT relative to the…

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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FSW-ALDH2-T2A-EGFP

Plasmid

#197036

Purpose

Express ALDH2 from hSyn1 promoter, co-expressed with EGFP

Depositor

Ronald Hart

Article

Sun et al Aging Cell. 2023 Feb;22(2):e13772. doi:

Insert

ALDH2 (ALDH2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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