We narrowed to 54,169 results for: plasmid

1. Dual opposing T7 promoters reporter plasmid

   Plasmid

   #156457

   Purpose

   A mutational reporter plasmid that can gain Kanamycin resistance upon mutation. Contains two T7 promoters flanking the kanamycin resistance gene.

   Depositor

   Matthew D. Shoulders

   Article

   Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

   Insert

   NeoR/KanR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   ATG start codon mutated to ACG

   Promoter

   Em7

   Available Since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PGK+ plasmid with onboard mCherry cassette

   Plasmid

   #169745

   Purpose

   PGK+ plasmid that also encodes separate mCherry cassette. mCherry is used to monitor plasmid dosage.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   eGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK-tetO2

   Available Since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. BcLOV4 fusion tools screening plasmid #1

   Plasmid

   #174511

   Purpose

   Cloning plasmid for creating BcLOV4 optogenetic tool fusions. Expresses [BamHI]-BcLOV4-[EcoRI]-GGGSx2-mCherry-[XhoI]-STOP in a pcDNA3.1 backbone.

   Depositor

   Brian Chow

   Article

   Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

   Insert

   [BamHI]-BcLOV4-[EcoRI]-GGGSx2-mCherry-[XhoI]-STOP
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. BcLOV4 fusion tools screening plasmid #2

   Plasmid

   #174512

   Purpose

   Cloning plasmid for creating BcLOV4 optogenetic tool fusions. Expresses [BamHI]-mCherry-[XhoI]-GGGSx2-BcLOV4-[EcoRI]-GGGS-3xFLAG-STOP in a pcDNA3.1 backbone.

   Depositor

   Brian Chow

   Article

   Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

   Insert

   plasmid B [BamHI]-mCherry-[XhoI]-GGGSx2-BcLOV4-[EcoRI]-GGGS-3xFLAG-STOP
   

   Use

   Tags

   3xFLAG and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Equalizer-H plasmid with onboard mCherry cassette

   Plasmid

   #169733

   Purpose

   Equalizer-H plasmid that encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   tetR-P2A-eGFP-miR(FF3) target-miR(FF3)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-tetO2

   Available Since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CMV+ plasmid with onboard mCherry cassette

   Plasmid

   #169741

   Purpose

   CMV+ plasmid that also encodes a separate mCherry cassette. mCherry is used to monitor plasmid dosage.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   eGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV-tetO2

   Available Since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLK88 - Edit-directing plasmid base

   Plasmid

   #98814

   Purpose

   Edit-directing plasmid base

   Depositor

   Leonid Kruglyak

   Insert

   CAN1.y gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   July 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Gr4-T2A-QF2 HDR plasmid

   Plasmid

   #140944

   Purpose

   Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti Gr4 gene

   Depositor

   Leslie Vosshall

   Article

   Jove et al Neuron. 2020 Dec 23;108(6):1163-1180.e12

   Insert

   Gr4-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Gr4-right-HDR-arm
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. RPS4X sgRNA-3 lentiCRISPR v2-Blast plasmid

   Plasmid

   #192233

   Purpose

   lentiviral vector expressing sgRNA-3 targeting eIF3 binding site of RPS4X 5'UTR

   Depositor

   Dieter Wolf

   Article

   Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

   Insert

   sgRNA-3 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. RPS15A sgRNA-5 lentiCRISPR v2-Blast plasmid

    Plasmid

    #192230

    Purpose

    lentiviral vector expressing sgRNA-5 targeting eIF3 binding site of RPS15A 5'UTR

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-5 targeting eIF3 binding site of RPS15A 5'UTR (RPS15A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. RPS4X sgRNA-4 lentiCRISPR v2-Blast plasmid

    Plasmid

    #192234

    Purpose

    lentiviral vector expressing sgRNA-4 targeting eIF3 binding site of RPS4X 5'UTR

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-4 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. RPS15A sgRNA-4 lentiCRISPR v2-Blast plasmid

    Plasmid

    #192229

    Purpose

    lentiviral vector expressing sgRNA-4 targeting eIF3 binding site of RPS15A 5'UTR

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-4 targeting eIF3 binding site of RPS15A 5'UTR (RPS15A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. fru-T2A-QF2 HDR plasmid

    Plasmid

    #141099

    Purpose

    Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti fru gene

    Depositor

    Leslie Vosshall

    Article

    Basrur et al Elife. 2020 Dec 7;9. pii: 63982. doi: 10

    Insert

    fru-left-HDR-arm; T2A-QF2-hsp70; 3xP3-dsRed-SV40; fru-right-HDR-arm
    

    Use

    Donor template

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDY153: Flag(SUZ12) WT HDR plasmid

    Plasmid

    #170793

    Purpose

    WT homology directed repair plasmid for performing genome-editing on the SUZ12 Locus

    Depositor

    Thomas Cech

    Article

    Youmans et al Mol Cell. 2021 Feb 4;81(3):488-501.e9. d

    Insert

    SUZ12 (SUZ12 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Equalizer-M plasmid with onboard mCherry cassette

    Plasmid

    #169734

    Purpose

    Equalizer-M plasmid that encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-tetO2

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Equalizer-L plasmid with onboard mCherry cassette

    Plasmid

    #169735

    Purpose

    Equalizer-L plasmid that encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    miR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-tetO2

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Ir7f-T2A-QF2 HDR plasmid

    Plasmid

    #140942

    Purpose

    Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti Ir7f gene

    Depositor

    Leslie Vosshall

    Article

    Jove et al Neuron. 2020 Dec 23;108(6):1163-1180.e12

    Insert

    Ir7f-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir7f-right-HDR-arm
    

    Use

    Tags

    Expression

    Mutation

    4 point mutations (annotated on plasmid map) were…

    Promoter

    Available Since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Ir7a-T2A-QF2 HDR plasmid

    Plasmid

    #140943

    Purpose

    Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti Ir7a gene

    Depositor

    Leslie Vosshall

    Article

    Jove et al Neuron. 2020 Dec 23;108(6):1163-1180.e12

    Insert

    Ir7a-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir7a-right-HDR-arm
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. B52 (empty plasmid backbone to express 2 sgRNAs)

    Plasmid

    #100708

    Purpose

    Empty plasmid backbone to express 2 sgRNAs (use BbsI and BsmBI for cloning)

    Depositor

    Alberto Ciccia

    Article

    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoters

    Available Since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. WT1-2A-eGFP PGK PuroR Donor Plasmid

    Plasmid

    #82333

    Purpose

    WT1 targeting donor

    Depositor

    Sean Palecek

    Article

    Bao et al Nat Biomed Eng. 2016;1. pii: 0003. doi:

    Inserts

    WT1 5' Arm (WT1 Human)
    WT1 3' Arm (WT1 )
    

    Use

    CRISPR and TALEN

    Tags

    2A-eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge