165,971 results

1. pcDNA3-Myc-AIM2

   Plasmid

   #73958

   Purpose

   Express c-Myc-tagged human AIM2 in mammalian cells

   Depositor

   Christian Stehlik

   Article

   Khare et al Nat Immunol. 2014 Apr;15(4):343-53. doi:

   Insert

   absent in melanoma 2 (AIM2) (AIM2 Human)
   

   Tags

   c-myc

   Expression

   Mammalian

   Promoter

   CMV iE

   Available Since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. qTAG-C-mScarlet-Blast

   Plasmid

   #207711

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-2A-Blast cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. Fibronectin-human-EDB in pMAX

   Plasmid

   #120403

   Purpose

   enables eukaryotic expression of human EDB (or EIIIB) containing fibronectin based on plasma fibronectin sequence

   Depositor

   Inaam Nakchbandi

   Article

   Kraft et al J Mol Med (Berl). 2016 May;94(5):567-81.

   Insert

   EDB-fibronectin (FN1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJWV102-PL-dCas9

   Plasmid

   #85588

   Purpose

   Integrate plasmid of Streptococcus pneumoniae, for chromosome integration of IPTG-inducible dCas9sp

   Depositor

   Jan-Willem Veening

   Article

   Liu et al Mol Syst Biol. 2017 May 10;13(5):931. do

   Insert

   dCas9sp
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   PLsp

   Available Since

   Jan. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJRH-1365 LV EF1a-CD28 IRES-EGFP

   Plasmid

   #201921

   Purpose

   2nd generation lentiviral transfer plasmid for engineering cells to express human CD28

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

   Insert

   CD28 (CD28 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-cMSP1E3D1

   Plasmid

   #199772

   Purpose

   Expresses split-intein fused MSP1E3D1 for circularization during expression (external His-Tag)

   Depositor

   Franz Hagn

   Article

   Daniilidis et al J Mol Biol. 2022 Dec 30;434(24):167861.

   Insert

   cMSP1E3D1
   

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   Extended cMSP1D1; external His-Tags flanking the …

   Available Since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-cofilin WT

   Plasmid

   #78295

   Purpose

   Mammalian expression of mouse cofilin fused to EGFP

   Depositor

   Michael Frotscher

   Article

   Chai et al Development. 2016 Mar 15;143(6):1029-40.

   Insert

   cofilin 1 (Cfl1 Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. PSMA4_GFP_Cterm

   Plasmid

   #221514

   Purpose

   Fluorescently tagged Psma4-GFP

   Depositor

   Alessandro Ori

   Insert

   PSMA4 (PSMA4 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. p2RZ

   Plasmid

   #27664

   Depositor

   Graeme Conn

   Article

   Walker et al Nucleic Acids Res. 2003 Aug 1. 31(15):e8

   Type

   Empty backbone

   Use

   Rna transcription

   Tags

   HDV Ribozyme

   Available Since

   April 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-hEF1A-MG-U6-sgRNA

    Plasmid

    #175505

    Purpose

    All-in-one piggyBac transposon destination vector for hEF1a expression of Mega Gate cloned elements and hU6 expression of Golden Gate cloned guide RNAs

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    hEF1a, U6

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-Limk1

    Plasmid

    #163414

    Purpose

    Expresses LIMK1 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Limk1 (Limk1 Mouse)
    

    Expression

    Mammalian

    Available Since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-cofilin-S3A

    Plasmid

    #78296

    Purpose

    Mammalian expression of mouse cofilin S3A (nonphosphorylatable form) fused to EGFP

    Depositor

    Michael Frotscher

    Article

    Chai et al Development. 2016 Mar 15;143(6):1029-40.

    Insert

    cofilin 1 (Cfl1 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S3A

    Promoter

    CAG

    Available Since

    May 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. sABE v3.22_N

    Plasmid

    #209553

    Purpose

    pCMV-bpSV40 NLS-TadA8e(2-76)-2xFRB-BGHpA

    Depositor

    Xue Gao

    Article

    Zeng et al Nat Commun. 2023 Sep 11;14(1):5573. doi:

    Insert

    TadA-8e(2-76)-2xFRB
    

    Expression

    Mammalian

    Promoter

    pCMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. sABE v3.22_C

    Plasmid

    #209554

    Purpose

    pCMV-SV40_NLS-2xFKBP3-TadA-8e(77-167)-SpCas9(D10A)-SV40_NLS-BGHpA

    Depositor

    Xue Gao

    Article

    Zeng et al Nat Commun. 2023 Sep 11;14(1):5573. doi:

    Insert

    2xFKBP-TadA-8e(77-167)-SpCas9(D10A)
    

    Expression

    Mammalian

    Promoter

    pCMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSV Sport PPAR gamma 2

    Plasmid

    #8862

    Depositor

    Bruce Spiegelman

    Article

    Tontonoz et al Genes Dev. 1994 May 15. 8(10):1224-34.

    Insert

    PPAR gamma (Pparg Mouse)
    

    Expression

    Mammalian

    Available Since

    Feb. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGTetRnls

    Plasmid

    #26599

    Depositor

    Peter Andrews

    Article

    Zafarana et al Stem Cells. 2009 Apr . 27(4):776-82.

    Insert

    Tetracycline repressor (TetR E.Coli)
    

    Tags

    nls

    Expression

    Mammalian

    Available Since

    Nov. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBBR2k-GFPuv

    Plasmid

    #106383

    Purpose

    Anhydrotetracycline-inducible tet promoter expressing gfpuv on BBR1 origin

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Insert

    GFPuv
    

    Expression

    Bacterial

    Promoter

    tet

    Available Since

    March 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. mEmerald-Vinexin-C-14

    Plasmid

    #54305

    Purpose

    Localization: Focal Adhesions, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Vinexin (SORBS3 Human)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

19. AAV-GfaABC1D-lck-smV5-4x6T-WPRE

    Plasmid

    #196416

    Purpose

    Astrocytic expression of membrane-targeted V5 spaghetti monster reporter in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

    Depositor

    Stanley Thomas Carmichael

    Article

    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

    Insert

    Lck-smV5
    

    Use

    AAV; Astrocyte-selective

    Tags

    Lck

    Expression

    Mammalian

    Promoter

    CAG and GfaABC1D

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. His-CL7_MBP_1xNLS_iGeoCas9(C2)_2xNLS(star)

    Plasmid

    #222991

    Purpose

    Bacterial expression of iGeoCas9(C2) construct

    Depositor

    Jennifer Doudna

    Article

    Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

    Insert

    His-CL7_MBP_1xNLS_iGeoCas9(C2)_2xNLS(star)
    

    Use

    CRISPR

    Tags

    CL7, His, and MBP

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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