162,699 results

1. Tn7 helper plasmid

   Plasmid

   #141161

   Purpose

   Temperature-sensitive plasmid carries tnsABCD genes that encode transposase biochemical machinery under an arabinose-inducible pBAD promoter

   Depositor

   Shimon Bershtein

   Article

   Jasinska et al Nat Ecol Evol. 2020 Feb 24. pii: 10.1038

   Insert

   Tn7 transposon machinery
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Arabinose-inducible pBAD

   Available since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT/TO-GAlphaQ-RLuc8

   Plasmid

   #140982

   Purpose

   Encodes a G alpha subunit (GNAQ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GAlphaQ-RLuc8 (GNAQ Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA3.1(+)-NEMOs

   Plasmid

   #189931

   Purpose

   Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

   Depositor

   Yubin Zhou

   Article

   Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

   Insert

   NEMOs
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE Flag HA MPG

   Plasmid

   #237804

   Purpose

   Lentiviral vector expressing WT MPG

   Depositor

   David Pellman

   Article

   Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

   Insert

   MPG (MPG Human)
   

   Use

   Tags

   Flag 3HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA3_cAMPFIRE-M

   Plasmid

   #182280

   Purpose

   Mammalian expression of the cAMP sensor cAMPFIRE-M under a CMV promotor.

   Depositor

   Haining Zhong

   Article

   Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

   Insert

   cAMPFIRE-M (cAMP sensor)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDG459

   Plasmid

   #100901

   Purpose

   SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

   Depositor

   Paul Thomas

   Article

   Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

   Inserts

   humanized CRISPR associated protein 9
   U6-gRNA scaffold 1
   U6-gRNA scaffold 2
   

   Use

   CRISPR and Mouse Targeting

   Tags

   3xFLAG and T2A-PuroR

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLIB

   Plasmid

   #80610

   Purpose

   pLIB - Library vector (biGBac system for expression of protein complexes in insect cells)

   Depositor

   Jan-Michael Peters

   Article

   Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti_EF1a_EV

   Plasmid

   #183116

   Purpose

   Empty vector control for pLenti_EF1a constructs

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   Small multiple cloning site
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHAGE-ERBB2

   Plasmid

   #116734

   Purpose

   Lentiviral expression of ERBB2

   Depositor

   Kenneth Scott, Gordon Mills

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   ERBB2 (ERBB2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pETM6

    Plasmid

    #49795

    Purpose

    ePathBrick vector for pathway engineering in E. coli.

    Depositor

    Mattheos Koffas

    Article

    Xu et al ACS Synth Biol. 2012 Jul 20;1(7):256-66.

    Type

    Empty backbone

    Use

    Tags

    S-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE-DDX3X

    Plasmid

    #116730

    Purpose

    Lentiviral expression of DDX3X

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    DDX3X (DDX3X Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. mCherry-OGG1

    Plasmid

    #215135

    Purpose

    Expresses mCherry-OGG1 in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    OGG1 (OGG1 Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCM157

    Plasmid

    #45863

    Depositor

    Mary Lidstrom

    Article

    Marx et al Biotechniques. 2002 Nov;33(5):1062-7.

    Insert

    cre recombinase
    

    Use

    Cre/Lox

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRISPRi_Mxi1_yl

    Plasmid

    #91248

    Purpose

    CRISPR-dCas9-Mxi1 vector for Yarrowia lipolytica, expressing dCas9-Mxi1 and AvrII site for sgRNA insertion

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.

    Inserts

    Codon optimized dCas9-Mxi1
    sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SCR1'-tRNA and UAS1B8-TEF(136)

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. htrpC6-YFP

    Plasmid

    #21084

    Depositor

    Craig Montell

    Article

    Kwon et al Mol Cell. 2007 Feb 23. 25(4):491-503.

    Insert

    TrpC6 (TRPC6 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBbB8k-csg-NbGFP

    Plasmid

    #166858

    Purpose

    Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a GFP-specific nanobody via a flexible linker. Derived from https://www.addgene.org/35363/.

    Depositor

    Neel Joshi

    Article

    Birnbaum et al Adv Sci (Weinh). 2021 May 3;8(11):200469

    Insert

    csgBACEFG, with GFP-specific nanobody fused to CsgA via flexible linker
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. mPiezo1-IRES-eGFP

    Plasmid

    #80925

    Purpose

    expresses mouse Piezo1 with GFP

    Depositor

    Ardem Patapoutian

    Article

    Coste et al Science. 2010 Oct 1;330(6000):55-60. doi

    Insert

    mouse Piezo1 CDS (Piezo1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR223-EBNA2

    Plasmid

    #195815

    Purpose

    Gateway Entry Clone of Epstein-Barr virus EBNA2

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    EBNA2 (EBNA-2 Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. INCTbiosyn-pUB-HspINTphiC31

    Plasmid

    #127518

    Purpose

    Plasmid encodes H. sapiens codon optimized Integrase phiC31.

    Depositor

    Martin Bonamino

    Article

    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

    Insert

    Integrase phiC31 coding sequence codon optimized for H. sapiens expression.
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCSDest-HA-TMPRSS2

    Plasmid

    #154963

    Purpose

    TMPRSS2 with N-terminal HA

    Depositor

    Utpal Pajvani

    Article

    Bartolome et al Sci Rep. 2021 May 7;11(1):9803. doi: 10.

    Insert

    HA-TMPRSS2 (TMPRSS2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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