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Showing: 2951 - 3000 of 22161 results

  7. pRSET a sfGCaMP6s-T78H

    Plasmid
    #100023
    Purpose
    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant containing T78H mutation
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    sfGCaMP6s-T78H
    Use
    Tags
    6x HIS tag and Xpress_EK tag
    Expression
    Bacterial
    Mutation
    cpEGFP replaced with superfolder cpGFP and T78H m…
    Promoter
    T7
    Available Since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100023-plasmid-map-sequence-id-194290

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  16. pcDNA3.1(+)-FluoSTEP-EKAR

    Plasmid
    #181858
    Purpose
    Genetically encoded FRET-based sensor for monitoring ERK activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.
    Depositor
    Jin Zhang
    Article
    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea
    Insert
    FluoSTEP-EKAR
    Use
    Tags
    mRuby2 and sfGFP(1-10)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    June 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    181858-plasmid-map-sequence-id-363410

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  19. pRSET a sfMatryoshCaMP6s

    Plasmid
    #100020
    Purpose
    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting superfolder cpGFP.
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    sfMatryoshCaMP6s
    Use
    Tags
    6x HIS tag and Xpress_EK tag
    Expression
    Bacterial
    Mutation
    cpEGFP replaced with superfolder cpGFP
    Promoter
    T7
    Available Since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100020-plasmid-map-sequence-id-194297

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  22. pMiNGo11-3

    Plasmid
    #126058
    Purpose
    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell
    Depositor
    Ralf-Bernd Klösgen
    Article
    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs
    Inserts
    mitochondria Rieske iron sulphur protein (1-100 aa)
    ccdB cassette (BsaI)
    GFP11x3 (5 aa linker)
    Use
    Binary
    Tags
    GFP1-10
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    126058-plasmid-map-sequence-id-254993

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  23. pMiNGo11-7

    Plasmid
    #126057
    Purpose
    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell
    Depositor
    Ralf-Bernd Klösgen
    Article
    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs
    Inserts
    mitochondria Rieske iron sulphur protein (1-100 aa)
    ccdB cassette (BsaI)
    GFP11x7 (5 aa linker)
    Use
    Binary
    Tags
    GFP1-10
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    126057-plasmid-map-sequence-id-246973

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  24. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_cpaA- Pconstitutive-sgRNA(Sth3)_blaA

    Plasmid
    #133347
    Purpose
    expression of two sgRNA from Streptococcus thermophilus #3 each express from its own constitutive promoter; here first one targets cpaA and second one targets blaA (from Caulobacter crescentus)
    Depositor
    Michael Laub
    Article
    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:
    Insert
    sgRNA_cpaA and sgRNA_blaA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    constitutive
    Available Since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    133347-plasmid-map-sequence-id-269540

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  26. pL6puro-JeT-pmRevErbA-miRFP713-NLS-Pest

    Plasmid
    #240110
    Purpose
    A lentiviral (infrared) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by synthtic JeT promoter.
    Depositor
    Achim Kramer
    Article
    Gabriel et al bioRxiv 2025.06.30.661926
    Insert
    murine Nr1d1 promoter+miRFP713-NLS-Pest-Reporter (Nr1d1 Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    added JeT Promoter
    Promoter
    Available Since
    Sept. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    240110-plasmid-map-sequence-id-485468

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  27. pL6puro-pSV40(3)-pmRevErbA-Venus-NLS-Pest

    Plasmid
    #240114
    Purpose
    A lentiviral (yellow) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by SV40 promoter fragment.
    Depositor
    Achim Kramer
    Article
    Gabriel et al bioRxiv 2025.06.30.661926
    Insert
    murine Nr1d1 promoter+Venus-NLS-Pest-Reporter (Nr1d1 Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    added SV40-Enhancer
    Promoter
    Available Since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    240114-plasmid-map-sequence-id-481774

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  28. pL6puro-pSV40(4)-pmRevErbA-Venus-NLS-Pest

    Plasmid
    #240115
    Purpose
    A lentiviral (yellow) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by SV40 promoter.
    Depositor
    Achim Kramer
    Article
    Gabriel et al bioRxiv 2025.06.30.661926
    Insert
    murine Nr1d1 promoter+Venus-NLS-Pest-Reporter (Nr1d1 Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    added SV40-Enhancer+Ori
    Promoter
    Available Since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    240115-plasmid-map-sequence-id-481765

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  29. pL6puro-pSV40(2)-pmRevErbA-Venus-NLS-Pest

    Plasmid
    #240113
    Purpose
    A lentiviral (yellow) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by SV40 promoter.
    Depositor
    Achim Kramer
    Article
    Gabriel et al bioRxiv 2025.06.30.661926
    Insert
    murine Nr1d1 promoter+Venus-NLS-Pest-Reporter (Nr1d1 Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    added SV40-Enhancer+Ori
    Promoter
    Available Since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    240113-plasmid-map-sequence-id-481756

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  30. pL6puro-pSV40(3)-pmRevErbA-mScaI3-NLS-Pest

    Plasmid
    #240117
    Purpose
    A lentiviral (red) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by SV40 promoter fragment.
    Depositor
    Achim Kramer
    Article
    Gabriel et al bioRxiv 2025.06.30.661926
    Insert
    murine Nr1d1 promoter+mScarlet-I3-NLS-Pest-Reporter (Nr1d1 Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    added SV40-Enhancer
    Promoter
    Available Since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    240117-plasmid-map-sequence-id-481757

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  31. pL6puro-pSV40(4)-pmRevErbA-mScaI3-NLS-Pest

    Plasmid
    #240118
    Purpose
    A lentiviral (red) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by SV40 promoter.
    Depositor
    Achim Kramer
    Article
    Gabriel et al bioRxiv 2025.06.30.661926
    Insert
    murine Nr1d1 promoter+mScarlet-I3-NLS-Pest-Reporter (Nr1d1 Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    added SV40-Enhancer+Ori
    Promoter
    Available Since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    240118-plasmid-map-sequence-id-481753

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  32. pL6puro-JeT-pmRevErbA-mClover3-NLS-Pest

    Plasmid
    #240109
    Purpose
    A lentiviral (yellow-green) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by synthtic JeT promoter.
    Depositor
    Achim Kramer
    Article
    Gabriel et al bioRxiv 2025.06.30.661926
    Insert
    murine Nr1d1 promoter+mClover3-NLS-Pest-Reporter (Nr1d1 Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    added JeT Promoter
    Promoter
    Available Since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    240109-plasmid-map-sequence-id-481752

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  33. pET30a-GABAT

    Plasmid
    #239572
    Purpose
    Expresses E. coli GABA transaminase (GABA-T/gabT) for recombinant protein purification. Contains a His6-tag on its C-terminus for Ni2+ affinity chromatography.
    Depositor
    William Plaxton
    Article
    Benidickson et al Biol Methods Protoc. 2025 Jun 20;10(1):b
    Insert
    4-aminobutyrate aminotransferase (gabT E. coli)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    July 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239572-plasmid-map-sequence-id-479014

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  34. pET30a-SSDH

    Plasmid
    #239573
    Purpose
    Expresses E. coli succinate semialdehyde dehydrogenase (SSDH/gabD) for recombinant protein purification. Contains a His6-tag on its C-terminus for Ni2+ affinity chromatography.
    Depositor
    William Plaxton
    Article
    Benidickson et al Biol Methods Protoc. 2025 Jun 20;10(1):b
    Insert
    Succinate-semialdehyde dehydrogenase (gabD E. coli)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    June 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    239573-plasmid-map-sequence-id-476594

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  36. pAAV GFP

    Plasmid
    #213685
    Purpose
    Expresses Green Fluorescent Protein (GFP) with an N-terminal twin-Strep tag
    Depositor
    Thomas Kukar
    Article
    Root et al Cell Rep. 2024 Nov 19;43(12):114985. doi
    Insert
    Twin-Strep GFP
    Use
    AAV
    Tags
    Twin-Strep
    Expression
    Mutation
    Promoter
    cytomegalovirus enhancer/chicken β-actin promoter
    Available Since
    Dec. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    213685-plasmid-map-sequence-id-455753

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  37. pDM097

    Plasmid
    #216819
    Purpose
    Aspergillus nidulans codon-adjusted mStayGold fluorescent protein, includes linker for N-terminal or internal tagging.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Insert
    mStayGold
    Use
    Tags
    FLAG-(SGGS)x2-XTEN16-(GGGGS)x3 and c4-(GGGGS)x2-X…
    Expression
    Bacterial
    Mutation
    Aspergillus nidulans codon-adjusted
    Promoter
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216819-plasmid-map-sequence-id-430144

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  44. pDY279

    Plasmid
    #182959
    Purpose
    Str resistant,CoE1* ori. CRISPR/Cas9 gRNA under constitutive J23119 promoter. gRNA targeting E. coli ptsI codon 2~9. HRT has synonymous mutations in ptsI. (for 1st round editing)
    Depositor
    Shelley Copley
    Article
    Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12
    Insert
    gRNA (ttcaggcattttagcatccc), homologous repair template for ptsI
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    182959-plasmid-map-sequence-id-365361

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  45. pDY281

    Plasmid
    #182960
    Purpose
    Amp resistant, CoE1* ori. CRISPR/Cas9 induced by AHTc, gRNA under constitutive J23119 promoter. gRNA targeting E. coli ptsI codon 2~9. HRT has synonymous mutations of ptsI. (for 2nd round editing)
    Depositor
    Shelley Copley
    Article
    Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12
    Insert
    gRNA (acctggtgaagccaatattc), homologous repair template for ptsI
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    182960-plasmid-map-sequence-id-365357

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  47. pASPIre4

    Plasmid
    #196656
    Purpose
    Derivative of pASPIre3. Contains SpeI restriction site within the CDS of bxb1 to enable diversification of the 5’-UTR and codons 2-16.
    Depositor
    Markus Jeschek
    Article
    Hollerer et al Nucleic Acids Res. 2023 Mar 21;51(5):237
    Insert
    Bxb1-sfGFP fusion controlled by rhamnose promoter; attB/attP-flanked discriminator; exchangable 5'-UTR and CDS (codons 1-16)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    SpeI site in Bxb1 CDS
    Promoter
    rhamnose-inducible promoter
    Available Since
    Aug. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    196656-plasmid-map-sequence-id-403168

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Showing: 2951 - 3000 of 22161 results