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OCT4-eGFP-2A-Puro

Plasmid

#31939

Depositor

Rudolf Jaenisch

Publication

Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

Inserts

OCT4 (POU5F1 Human)
eGFP (eGFP )

Use

Targeting donor

Tags

eGFP

Available since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pET_RTX

Plasmid

#102787

Purpose

Expresses the Reverse Transcriptase gene

Depositor

Andrew Ellington

Publication

Ellefson et al Science. 2016 Jun 24;352(6293):1590-3. d

Insert

reverse transcription xenopolymerase

Expression

Bacterial

Promoter

T7 promoter + lac operator

Available since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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APEX2-GBP

Plasmid

#67651

Purpose

Mammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody).

Depositor

Rob Parton

Publication

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

APEX2-GBP

Expression

Mammalian

Promoter

CMV

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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FU-tet-o-hOct4

Plasmid

#19778

Depositor

Konrad Hochedlinger

Publication

Maherali et al Cell Stem Cell. 2008 Sep 11. 3(3):340-5.

Insert

Oct4 (POU5F1 Human)

Use

Lentiviral

Expression

Mammalian

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pT7-PE7 for IVT

Plasmid

#214813

Purpose

Template for in vitro transcription of PE7

Depositor

Brittany Adamson

Publication

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7

Use

Template for in vitro transcription

Tags

SV40 bpNLS and c-Myc NLS

Promoter

T7 (inactivated)

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-cFos-tTA-pA

Plasmid

#66794

Purpose

Expresses TetActivator protein under the control of the c-fos promoter for use in TetTaging

Depositor

William Wisden

Publication

Zhang et al Nat Neurosci. 2015 Apr;18(4):553-61. doi

Insert

cfos promoter linked to the tet activator gene

Use

AAV

Tags

no

Expression

Mammalian

Available since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pAAV-GFAP104-mCherry

Plasmid

#58909

Purpose

AAV-mediated expression of mCherry under the GFAP promoter

Depositor

Edward Boyden

Publication

Perea et al Nat Commun. 2014;5:3262. doi: 10.1038/nc

Has service

AAV5

Insert

mCherry

Use

AAV

Expression

Mammalian

Promoter

GFAP

Available since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pHR CD19-FcGamma CAR

Plasmid

#113014

Purpose

for production of lentivirus encoding the CD19 CAR-Pfcgamma

Depositor

Ron Vale

Publication

Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

Insert

CD19 CAR-P intracellular GFP (CD19 Mouse, Synthetic, Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-hDlx-GiDREADD-dTomato-Fishell-5

Plasmid

#83896

Purpose

Gi-DREADD (P2A) nuclear dTomato expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer element

Depositor

Gordon Fishell

Publication

Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

Has service

AAV1, AAV9, and AAV Retrograde

Insert

hM4Di

Use

AAV

Expression

Mammalian

Promoter

hDlx

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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psfaRb-Tn5

Plasmid

#183638

Purpose

Expresses anti-rabbit nanobody fused with Tn5

Depositor

Goncalo Castelo-Branco

Publication

Bartosovic et al Nat Biotechnol. 2023 Jun;41(6):794-805.

Insert

anti rabbit secondary nanobody fused to Tn5

Expression

Bacterial

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA

Plasmid

#50943

Purpose

Bicistronic vector expressing mRuby2 and GCaMP6f from a single open reading frame.

Depositor

Tobias Bonhoeffer, Mark Huebener, Tobias Rose

Publication

Rose et al Science. 2016 Jun 10;352(6291):1319-22.

Insert

mRuby2-P2A-GCaMP6f

Use

AAV

Promoter

hSyn1

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

1 Data Hub Report

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pDonor_mU6

Plasmid

#69350

Purpose

sgRNA scaffold and mouse U6 promoter

Depositor

Andrea Ventura

Publication

Vidigal et al Nat Commun. 2015 Aug 17;6:8083. doi: 10.

Insert

mU6 promoter and sgRNA scaffold flanked by BbsI sites

Use

CRISPR

Promoter

mU6 promoter

Available since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEvolvR-enCas9-PolI5M

Plasmid

#113078

Purpose

Expresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

Depositor

John Dueber, David Schaffer

Publication

Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

Insert

enCas9-PolI5M

Use

CRISPR

Expression

Bacterial

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DO_DIO-TdTomato_EGFP-WPRE-pA

Plasmid

#37120

Purpose

DO/DIO Cre-Switch. Expresses tdTomato in Cre negative cells, expresses EGFP in Cre positive cells

Depositor

Bernardo Sabatini

Publication

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

TdTomato-EGFP

Use

AAV and Cre/Lox; Cre-switch

Promoter

EF1a

Available since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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pSECC

Plasmid

#60820

Purpose

3rd generation vector. Expresses a sgRNA of interest, Cas9 and Cre

Depositor

Tyler Jacks

Publication

Sanchez-Rivera et al Nature. 2014 Oct 22. doi: 10.1038/nature

Inserts

Cas9
Cre

Use

CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

Tags

Flag

Expression

Mammalian

Mutation

BsmBI site eliminated by C->A; E308G

Promoter

EFS and EFS (after Cas9-2A)

Available since

Nov. 19, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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pAAV-DJ-N589X

Plasmid

#130878

Purpose

Encodes the AAV-DJ capsid with the N589 residue replaced with a TAG stop codon

Depositor

Prashant Mali

Publication

Katrekar et al Sci Rep. 2018 Feb 26;8(1):3589. doi: 10.

Insert

AAV-DJ cap N589X

Use

AAV

Expression

Mammalian

Mutation

N589X

Promoter

Unknown

Available since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pCAG-iCre

Plasmid

#89573

Purpose

Expresses iCre under pCAG

Depositor

Wilson Wong

Publication

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

iCre

Use

Cre/Lox and Synthetic Biology

Tags

NLS

Expression

Mammalian

Available since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pSB700

Plasmid

#64046

Purpose

Lentiviral vector for expressing U6 sgRNA and CAGGS Cerulean fluorescent protein

Depositor

George Church

Publication

Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

Type

Empty backbone

Use

CRISPR, Lentiviral, and Synthetic Biology

Expression

Mammalian

Promoter

U6, CAGGS

Available since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pTP396

Plasmid

#149336

Purpose

Bacterial expression of His14-Biotin_acceptor_peptide-SUMOEu1-anti-GFP_nanobody

Depositor

Rebecca Voorhees

Publication

Pleiner et al Science. 2020 May 21. pii: science.abb50

Insert

anti-GFP nanobody

Tags

Biotin acceptor peptide (Avi-tag), His14, and SUM…

Expression

Bacterial

Available since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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Snail_pGL2

Plasmid

#31694

Depositor

Paul Wade

Publication

Fujita et al Cell. 2003 Apr 18. 113(2):207-19.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Snail (SNAI1 Human)

Use

Luciferase

Expression

Mammalian

Available since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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