Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 92,266 results for: lias

Clear keywords
Showing: 11981 - 12000 of 92266 results

  3. TDP-REGv2(Gaussia Luciferase)#5 in pTwist-CMV

    Plasmid
    #216164
    Purpose
    Expression of secreted Gaussia Luciferase specifically in cells with TDP-43 loss of function. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    Gaussia princeps luciferase (with double mutation to improve stability)
    Expression
    Mammalian
    Available Since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216164-plasmid-map-sequence-id-437759

    Enlarge

  6. pPBbsr2-AREG-ScNeo

    Plasmid
    #209910
    Purpose
    To monitor the status of Amphiregulin, the plasmid encodes a recombinant AREG fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
    Depositor
    Michiyuki Matsuda
    Article
    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi
    Insert
    AREG-ScNeo (AREG Human)
    Tags
    mNeonGreen and mScarlet
    Expression
    Mammalian
    Available Since
    Nov. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    209910-plasmid-map-sequence-id-416693

    Enlarge

  15. pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iPA

    Plasmid
    #134925
    Purpose
    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iPA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses PuroR protein.
    Depositor
    Tomoji Mashimo
    Article
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Insert
    Cascade factors (Cse2(Cas11), Cas3, Cas6) with bpNLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134925-plasmid-map-sequence-id-267605

    Enlarge

  16. pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iPA

    Plasmid
    #134924
    Purpose
    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iPA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses PuroR protein.
    Depositor
    Tomoji Mashimo
    Article
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Insert
    Cascade factors (Cse1(Cas8), Cas5,Cas7) with bpNLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134924-plasmid-map-sequence-id-267600

    Enlarge

  17. pCC_08 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dxCas9NG-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139093
    Purpose
    Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dxCas9-NG-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139093-plasmid-map-sequence-id-273890

    Enlarge

  18. pMXS-mitoGshF-blasticidin

    Plasmid
    #210314
    Purpose
    Expresses mitochondrially targeted, humanized Streptococcus thermophilus GshF in mammalian cells
    Depositor
    Kivanc Birsoy
    Article
    Wang et al Nature. 2021 Nov;599(7883):136-140. doi:
    Insert
    Bifunctional glutamate-cysteine ligase/glutathione synthetase GshF (gshAB Streptococcus thermophilus)
    Use
    Retroviral
    Tags
    FLAG peptide, Homo sapiens ACO2 mitochondrial tar…
    Mutation
    Codon optimized for expression in human cells
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    210314-plasmid-map-sequence-id-418848

    Enlarge

  20. ssAAV-EF1a-FLuc-WPRE-HgHpA_bac_293

    Plasmid
    #118412
    Purpose
    Expresses firefly luciferase under the control of a strong ubiquitous promoter EF1a along with a WPRE cassette. Backbone is compatible with both baculoviral and human production methods of AAV.
    Depositor
    Nicole Paulk
    Article
    Rumachik et al Mol Ther Methods Clin Dev. 2020 May 22;1
    Insert
    Firefly luciferase
    Use
    AAV, Luciferase, and Synthetic Biology
    Expression
    Insect and Mammalian
    Promoter
    EF1a
    Available Since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    118412-plasmid-map-sequence-id-231800

    Enlarge

Showing: 11981 - 12000 of 92266 results