161,123 results

1. pGL3-U6-sgRNA-PGK-puromycin

   Plasmid

   #51133

   Purpose

   expresses sgRNA from the U6 promoter.

   Depositor

   Xingxu Huang

   Article

   Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCW-eGFP

   Plasmid

   #162823

   Purpose

   Doxycycline-inducible overexpression of eGFP

   Depositor

   Matthew Steinhauser

   Article

   Fazeli et al Mol Metab. 2020 Sep 28;42:101082. doi: 1

   Insert

   eGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   V163A

   Promoter

   Tet ON

   Available since

   Feb. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTAT-HA

   Plasmid

   #35612

   Depositor

   Steven Dowdy

   Article

   Nagahara et al Nat Med. 1998 Dec;4(12):1449-52.

   Type

   Empty backbone

   Use

   Tags

   6xHIS, HA, and TAT

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-28a (+)-hTBP

   Plasmid

   #171073

   Purpose

   Expresses human TBP in bacteria. The resulting plasmid was used to generate a single expression construct encoding a His-tagged TBP

   Depositor

   Joan Conaway

   Article

   He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

   Insert

   TATA-box-binding protein (TBP Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. GRK2-CAAX

   Plasmid

   #166224

   Purpose

   GRK2-CAAX mammalian expression plasmid

   Depositor

   Robert Lefkowitz

   Article

   Inglese et al Nature. 1992 Sep 10;359(6391):147-50. do

   Insert

   bovine GRK2-CAAX (GRK2 Bovine)
   

   Use

   Tags

   no

   Expression

   Mammalian

   Mutation

   adding CAAX sequence at C terminal

   Promoter

   CMV

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. FUW-Luc-mCherry-puro

   Plasmid

   #183685

   Purpose

   Lentiviral vector expressing luciferase and mCherry.

   Depositor

   Andrew Kung

   Article

   Kimbrel et al Mol Imaging. 2009 May-Jun;8(3):140-7.

   Insert

   Luc-E2A-mCherry-T2A-PuroR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Human Ubiquitin C (UbC) promoter

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET15-UbE2W

   Plasmid

   #15809

   Depositor

   Wade Harper

   Article

   Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

   Insert

   Ubiquitin conjugating enzyme UbE2W (UBE2W Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX6P1-DEST

   Plasmid

   #119749

   Purpose

   Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag.

   Depositor

   Andrew Jackson, Martin Reijns

   Type

   Empty backbone

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. AiO-Cas12a: All in one enAsCas12a expression and gRNA cloning backbone

   Plasmid

   #228364

   Purpose

   mU6-driven expression of enAsCas12a gRNAs with EF1a-enAsCas12a-2A-Puro

   Depositor

   Michael Boettcher

   Article

   El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral; Enascas12a nuclease and en…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6

   Available since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLQ9834_sgBB: pHR-hU6-CasMINI sgRNA_#2; EF1a-Puro-T2A-BFP- WPRE

    Plasmid

    #180280

    Purpose

    The CasMINI sgRNA cloning backbone with sites 'BsmBI' for inserting new guide sequences.

    Depositor

    Stanley Qi

    Article

    Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

    Insert

    CasMINI sgRNA (backbone) and BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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