12,601 results

1. Talin1-mCherry

   Plasmid

   #212004

   Purpose

   Expression of mouse full-length (1-2541) talin1-mCherry in mammalian cells

   Depositor

   Vesa Hytönen

   Article

   Rahikainen et al J Cell Sci. 2019 Apr 3;132(7):jcs226514.

   Insert

   Mouse talin1 (Tln1 Mouse)
   

   Use

   Tags

   mCherry fluorescent protein

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmCherry-ATG5

   Plasmid

   #13095

   Depositor

   Roberta Gottlieb

   Article

   Hamacher-Brady et al Cell Death Differ. 2007 Jan;14(1):146-57

   Insert

   ATG5 (Atg5 Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. mu-FLT3Lv1-pTRIP

   Plasmid

   #163519

   Purpose

   Used to construct lentivirus to express murine FLT3 ligand (variant-1) in mammalian cells

   Depositor

   Thomas Weber

   Insert

   murine-FLT3L variant 1 (Flt3l Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-rGBD

   Plasmid

   #26732

   Depositor

   William Bement

   Article

   Benink et al J Cell Biol. 2005 Jan 31. 168(3):429-39.

   Insert

   rhotekin (rtkn2 Mouse)
   

   Use

   Reporter for rho

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   RhoA binding domain of rhotekin

   Promoter

   Available since

   Feb. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA4/TO 3xFlag-m-PKD1-2xMyc/His

   Plasmid

   #83452

   Purpose

   Full-length murine PC1 for Eukaryotic Expression (982)

   Depositor

   Thomas Weimbs

   Article

   Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

   Insert

   polycystin-1 (Pkd1 Mouse)
   

   Use

   Tags

   2xMyc, 3xFlag, and 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPB-tetO(hCMV1)-HA-Tet1mHxD(201R2)-IV

   Plasmid

   #102422

   Purpose

   Inducible expression of siRNA resistant mouse Tet1-201 (ENSMUST00000050826.13) with mutated catalytic domain (H1620Y & D1622A), HA-tag and IRES-Venus in piggyBac (PB) transposon vector

   Depositor

   Kian Peng Koh

   Article

   Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi

   Insert

   Tet1 catalytic domain mutant (Tet1 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   H1620Y and D1622A mutations in Tet1 catalytic dom…

   Promoter

   TetO-CMV

   Available since

   Nov. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1+FLAG-KrascomG12D

   Plasmid

   #206844

   Purpose

   To express mouse Kras encoded by common mouse codons (to increase expression) and with a G12D mutation with an N-terminal FLAG tag.

   Depositor

   Christopher Counter

   Article

   Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755

   Insert

   Kras (Kras Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Codons altered to most optimum based on mouse cod…

   Promoter

   Available since

   Oct. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDEST47-Arl13b12

   Plasmid

   #40872

   Depositor

   Tamara Caspary

   Article

   Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

   Insert

   Arl13b (Arl13b Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   no stop codon

   Promoter

   CMV

   Available since

   Nov. 5, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3-mouse PKA-RIIalpha-mEGFP

   Plasmid

   #45527

   Depositor

   Haining Zhong

   Article

   Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

   Insert

   PKA regulatory subunit RII alpha tagged by mEGFP
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR-hSyn-EGFP

    Plasmid

    #114215

    Purpose

    Lentiviral vector (control) that targets expression of EGFP to neurons

    Depositor

    Xue Han

    Article

    Keaveney et al Cell Rep. 2018 Jul 10;24(2):294-303. doi

    Insert

    Enhanced Green Fluorescent Protein
    

    Use

    Lentiviral and Mouse Targeting

    Tags

    None

    Expression

    Mammalian

    Mutation

    None

    Promoter

    human synapsin (hSyn)

    Available since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRN3P-HA-Suv4-20h1mut

    Plasmid

    #86690

    Purpose

    For transcription of of Suv4-20h1mut mRNA preceded by an HA-tag in 5'

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Eid et al Genes Dev. 2016 Nov 15;30(22):2513-2526.

    Insert

    histone-lysine N-methyltransferase KMT5B mutant (Kmt5b Mouse)
    

    Use

    Tags

    HA

    Expression

    Mutation

    mutated region NHDC (asparagine 273 to cysteine 2…

    Promoter

    T3

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CMV-BDNF-Myc

    Plasmid

    #124279

    Purpose

    Express Myc-tagged brain-derived neurotrophic factor (BDNF)

    Depositor

    Baoji Xu

    Article

    Xie et al Cell Metab. 2019 Jan 11. pii: S1550-4131

    Insert

    BDNF (Bdnf Mouse)
    

    Use

    AAV

    Tags

    Myc

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAd-M3cherry

    Plasmid

    #61041

    Purpose

    This plasmid mediates polycistronic expression of 4 genes: Ngn3, Pdx1, Mafa, and mCherry. It can be used to convert mouse pancreatic acinar cells to induced beta-like cells in vivo.

    Depositor

    Qiao Zhou

    Article

    Li et al Nat Biotechnol. 2014 Dec;32(12):1223-30.

    Inserts

    Ngn3 (Neurog3 Mouse)
    pdx1 (Pdx1 Mouse)
    Mafa (Mafa Mouse)
    monomeric cherry
    

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMC1SG5

    Plasmid

    #31282

    Depositor

    Aziz Sancar

    Article

    Ye et al J Biol Chem. 2011 Jul 22;286(29):25891-9

    Insert

    Cry1 (Cry1 Mouse)
    

    Use

    Tags

    Flag, His, and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pCAGGS-FLAG-CaMKIIA

    Plasmid

    #186825

    Purpose

    Expresses FLAG-CaMKIIA in mammalian cells

    Depositor

    Hung-Chun Chang

    Article

    Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

    Insert

    Camk2a (Camk2a Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRN3P-HA-Suv4-20h2mut

    Plasmid

    #86692

    Purpose

    For transcription of of Suv4-20h2mut mRNA preceded by an HA-tag in 5'

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Eid et al Genes Dev. 2016 Nov 15;30(22):2513-2526.

    Insert

    histone-lysine N-methyltransferase KMT5C mutant (Kmt5c Mouse)
    

    Use

    Tags

    HA

    Expression

    Mutation

    Mutated asparagine 273 to cysteine 276 (NHDC) int…

    Promoter

    T3

    Available since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPB_TRE3G_scFV-sfGFP-Kmt5cFL-CatMut

    Plasmid

    #235590

    Purpose

    Dox-inducible expression of control catalytic-mutant Kmt5c CD fused with scFV

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    Kmt5c (Kmt5c Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    NHDC182-185AAAG

    Promoter

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pB-3xFlag-dCas13-mTET2HxDCD

    Plasmid

    #228235

    Purpose

    For targeted tethering of an inactive mutant (HxD) of the catalytic domain of mouse TET2 using dCas13

    Depositor

    Chuan He

    Article

    Zou et al Nature. 2024 Oct 2. doi: 10.1038/s41586-

    Inserts

    dCas13
    TET2 (Tet2 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    CD (cysteine-rich, dioxygenase domain) truncation…

    Promoter

    CAG

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLL5.0 shFmnl2-EGFP

    Plasmid

    #187255

    Purpose

    Lentiviral expression of shRNA targeting Fmnl2

    Depositor

    Alpha Yap

    Article

    Katsuno-Kambe et al Biol Cell. 2021 Feb;113(2):107-117. doi:

    Insert

    ShRNA for Fmnl2 (Fmnl2 Mouse)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    U6

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEN515 - pTRE3G-CTCF(ZFmut)-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE

    Plasmid

    #156434

    Purpose

    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible mutant CTCF mouse cDNA (all Zinc fingers point mutated), targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA.

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    All zinc fingers mutated

    Promoter

    Available since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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