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Plasmid#46765PurposeE.coli genomic integration rbsAR locusDepositorTypeEmpty backboneUseE.coli genomic integrationTagsExpressionMutationPromoterAvailable sinceSept. 23, 2013AvailabilityAcademic Institutions and Nonprofits only
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pBTK622
Plasmid#180592Purposetdk/kanR dual selection cassette for engineering the genome of Acinetobacter baylyi ADP1DepositorInsertstdk
kanR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pTKDP-neo
Plasmid#71320PurposeDonor plasmid - kanamycin resistant donor cassetteDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLG016 (ApeA)
Plasmid#157894PurposeExpresses the ApeA defense systemDepositorInsertApeA (putative HEPN RNase)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHEA
Plasmid#168297PurposeBacterial surface display of polypeptides (nanobodies) in E. coliDepositorInsertEnterohemorraghic E. coli autotransporter EhaA C-terminal fragment (C-EhaA)
UseTags6xHis, E-tag, and PelB signal peptideExpressionBacterialMutationPromoterLacI-PlacAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKIKOrbsARKm
Plasmid#46768PurposeE.coli genomic integration rbsAR locusDepositorTypeEmpty backboneUseE.coli genomic integrationTagsExpressionMutationPromoterAvailable sinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTarget12f1
Plasmid#171183PurposeConstitutive expression of sgRNA without donor DNADepositorInsertsgRNA scaffold in the CRISPR/Cas12f1 system
UseCRISPRTagsExpressionBacterialMutationPromoterJ23119(SpeI) promoterAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMUT2-pLac-mCherry
Plasmid#192859PurposepMUT2 based vector for sensing lactic acid in E. coli Nissle 1917DepositorInsertlldR operon
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBbA5c-MevT-MBI (JBEI-3085)
Plasmid#87950Purposethe overproduction of the universal building blocks to make isoprenoids (i.e. IPP and DMAPP).DepositorInsertMevT-MBI
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAJM.683
Plasmid#108531PurposeBetIAM + PBetI-YFP Reporter. Cho sensor for use in non-Marionette strain.DepositorInsertBetIAM + PBetI-YFP
UseTagsExpressionBacterialMutationBetI: S115IPromoterAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDsEndoNA2
Plasmid#174728PurposeExpresses DsRed-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.DepositorInsertDsRed-Monomer
UseTagsHistagExpressionBacterialMutationPromoterT5Available sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMH062
Plasmid#225537PurposeExpresses IS621 in insect cellsDepositorInsertIS621
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a T7pCONS sfGFP
Plasmid#154462PurposepET28a with a design flaw in the T7 promoter fixed. Contains the coding sequence for sfGFPDepositorInsertsuper folder GFP
UseTagsHis-thrombinExpressionBacterialMutationPromoterT7Available sinceAug. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAGD236
Plasmid#219497PurposepCDF-Duet deriviative with sfGFP flanked by 2 BsaI sites downstream of the first T7 promoter and independent msr and RT downstream of the second T7 promoterDepositorInsertsfGFP, Eco1 msr, Eco1 RT
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT2SK
Plasmid#59383PurposeTemplate plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a kanamycin resistance gene.DepositorInsertTT-ISceI-kan
UseTemplateTagsExpressionMutationPromoterAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pT2SC
Plasmid#59382PurposeTemplate plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a chloramphenicol resistance gene.DepositorInsertTT-ISceI-cat
UseTemplateTagsExpressionMutationPromoterAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMDICI
Plasmid#51652PurposeE coli genetic modificationDepositorInsertCmR
UseGenetic modificationTagsExpressionMutationPromoterunknownAvailable sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAJM.715
Plasmid#108516PurposePTac-YFP Reporter. IPTG sensor for use in Marionette strain.DepositorInsertPTac-YFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRF0G-Tyr
Plasmid#113644PurposePlasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging tyrosine at amber stop codons.DepositorInsertMjYRS and tRNA gene
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
PET-3a-chpBK
Plasmid#155201PurposeTo express recombinant ChpBK proteinDepositorInsertschpS-chpBK-6×His-Tag
GyrA-CBD-10xHis-Tag
UseTags10xHis and 6xHisExpressionBacterialMutationPromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCH1 - pBait-empty
Plasmid#174663Purposearabinose-induced synthesis of an RNA containing a single MS2 hairpin. Used as a three-hybrid control. For cloning purposes, see plasmid #174662.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMYO127TAG-SepT
Plasmid#34625DepositorInsertMyoglobin-127TAG
UseTags6xHISExpressionBacterialMutationcodon-optimized + an amber stop codon was introdu…PromoterIppAvailable sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBAD-6xHis-BglII
Plasmid#196654PurposeDerived from pGLO (BioRad) by the insertion of a 6xHis Tag and a BglII siteDepositorTypeEmpty backboneUseTags6xHis tagExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
p5E-QUASM1
Plasmid#155114Purpose5' entry vector containing QUASM1 promoter element.DepositorInsertQUASM1
UseGateway 5' entry vectorTagsExpressionMutationPromoterQUASM1-E1bAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET30b_T7Sav
Plasmid#138588PurposeT7-tagged streptavidin with native C-terminus controlled by PT7 promoter; KanR, pBR322 oriDepositorInsertT7-tagged streptavidin variant with native C-terminus
UseSynthetic BiologyTagsT7-tagExpressionBacterialMutationPromoterPT7Available sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAJM.717
Plasmid#108517PurposePTet*-YFP Reporter. aTc sensor for use in Marionette strain.DepositorInsertPTet*-YFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZH516
Plasmid#102669PurposeConstitutive expression of GFPmut2; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAJM.712
Plasmid#108513PurposePCymRC-YFP Reporter. Cuma sensor for use in Marionette strain.DepositorInsertPCymRC-YFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAJM.716
Plasmid#108518PurposePBAD-YFP Reporter. Ara sensor for use in Marionette strain.DepositorInsertPBAD-YFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only