We narrowed to 3,273 results for: BID;

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pRPL28(K55M) SpHIS5 (pNTI666)

Plasmid

#115436

Purpose

Expresses K55M RPL28 from HIS3 yeast plasmid

Depositor

Nicholas Ingolia

Article

McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

Inserts

RPL28 (RPL28 Budding Yeast)
HIS5

Expression

Yeast

Mutation

K55M; MfeI site in intron

Promoter

Ashbya gospii TEF1 and endogenous

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCDF-NtXNtTcR1AtR2NtB2

Plasmid

#160889

Purpose

Expression of N. tabacum Rubisco small subunit (rbcS-Tc), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

PT7-Nt-rbcS-Tc

Expression

Bacterial

Promoter

rbcS-Tc: T7, chaperones: T12

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCDF-NtXR1AtR2B2NtS1

Plasmid

#160879

Purpose

Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1 and A. thaliana Rubisco chaperone proteins raf2, and bsd2 in E. coli.

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

rbcS-S1, rbcX, raf1, raf2, bsd2

Expression

Bacterial

Promoter

rbcS-S1: pBAD, chaperones: T7

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pRW006-SM-destination-pro35S-Cas9

Plasmid

#104439

Purpose

Destination vector expressing plant-codon-optimized Cas9 under 35S promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free

Depositor

Detlef Weigel

Article

Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

Type

Empty backbone

Use

CRISPR

Expression

Bacterial and Plant

Promoter

35S

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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PhyB-mCherry-Htb2

Plasmid

#51569

Purpose

Constitutively express nuclear localized PhyB in Saccharomyces cerevisiae

Depositor

Chao Tang

Article

Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

Insert

PhyB (PHYB Mustard Weed)

Tags

HTB2 and mCherry

Expression

Yeast

Mutation

1-908aa only

Promoter

ADH1pr

Available Since

Aug. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pDGE652

Plasmid

#153230

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pTYF-1xSYN-EGFP

Plasmid

#19973

Depositor

Sergey Kasparov

Article

Liu et al BMC Biotechnol. 2008 May 16;8:49. doi: 1

Insert

SYN-EGFP-WPRE (SYN1 jellyfish, Human)

Expression

Mammalian

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

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NLS-GAI(1-92)

Plasmid

#37317

Depositor

Takanari Inoue

Article

Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

Insert

GAI (GAI Mustard Weed)

Tags

NLS

Expression

Mammalian

Mutation

contains aa 1-92

Promoter

CMV

Available Since

June 15, 2012

Availability

Academic Institutions and Nonprofits only
pAFMW-AthHEN1

Plasmid

#104980

Purpose

Expression of FLAG Myc tagged A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

Insert

Hen1 (HEN1 Mustard Weed)

Tags

FLAG Myc

Expression

Insect

Promoter

Actin5C

Available Since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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CFP-GAI(1-532)

Plasmid

#37310

Depositor

Takanari Inoue

Article

Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

Insert

GAI (GAI Mustard Weed)

Tags

CFP

Expression

Mammalian

Mutation

contains aa 1-532

Promoter

CMV

Available Since

June 15, 2012

Availability

Academic Institutions and Nonprofits only
pHA-ΔN335-Kin1A

Plasmid

#31606

Depositor

Anthony Brown

Article

Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-50

Insert

ΔN335 kinesin-1A (Kif5a Mouse)

Tags

HA-tag

Expression

Mammalian

Mutation

deleted amino acids 1-335

Promoter

CMV

Available Since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only
pU6-26 (GB1001)

Plasmid

#68208

Purpose

Provides the A. thaliana U6-26 RNA polIII promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtU6-26 promoter

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pCDF-NtXNtT2R1AtR2NtB2

Plasmid

#160886

Purpose

Expression of N. tabacum Rubisco small subunit (rbcS-T2), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

PT7-Nt-rbcS-T2

Expression

Bacterial

Promoter

rbcS-T2: T7, chaperones: T9

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS-CRY2-mCherry

Plasmid

#178525

Purpose

Expresses CRY2-mCherry in mammalian cells

Depositor

Kazuhiro Aoki

Article

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

CRY2-mCherry

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR_L1-BirA(R118G)-YFP-NLS-STOP-L2

Plasmid

#127360

Purpose

gateway entry vector for expressing nuclear BirA* (R118G)-YFP under a promoter of choice

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

BirA* (R118G mutant)

Use

Gateway entry vector

Tags

GS linker, NLS, V5, and YFP

Mutation

R118G

Promoter

no promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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optoPKAW196R

Plasmid

#104548

Purpose

Expresses Cry2-mCherry-PKA W196R in mammalian cells

Depositor

David Lawrence

Article

O'Banion et al Cell Chem Biol. 2017 Oct 25. pii: S2451-

Insert

Cry2-mCherry-PKA W196R

Tags

mCherry

Expression

Mammalian

Mutation

PKA W196R

Promoter

CMV

Available Since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

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pSLQ2804 pHR: U6-SpsgTRE3G CMV-PYL1-VPR-IRES-mCherry

Plasmid

#84258

Purpose

Expresses Sp sgTRE3G gRNA with ABA-inducible VPR and mCherry for OR gate

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

Sp sgTRE3G
PYL1-VPR

Use

CRISPR and Lentiviral

Tags

IRES-mCherry and PYL1

Expression

Mammalian

Promoter

CMV and mouse U6

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pEG302 22aa SunTag NtDRMcd (no NLS) SUP

Plasmid

#115490

Purpose

CRISPR-Cas9 SunTag system to target NtDRMcd (without an NLS) to the SUPERMAN locus with two guide RNAs

Depositor

Steven Jacobsen

Article

Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

Insert

g2_U6_g1_U6_NOS_NLS_GB1_noNLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR_L1-BirA(R118G)-NES-YFP-STOP-L2

Plasmid

#127357

Purpose

gateway entry vector for expressing cytosolic BirA* (R118G)-YFP under a promoter of choice

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

BirA* (R118G mutant)

Use

Gateway entry vector

Tags

GS linker, NES, V5, and YFP

Mutation

R118G

Promoter

no promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pKan-PCUP1-AID*(N)

Plasmid

#99525

Purpose

N-terminal AID* degron cassette with CUP1 promoter and KanR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-117) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Promoter

CUP1

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pDGE671

Plasmid

#153237

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Tags

GFP

Expression

Plant

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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PhyB-mCherry-SIK1

Plasmid

#51577

Purpose

Constitutively express nucleolus localized PhyB in Saccharomyces cerevisiae

Depositor

Chao Tang

Article

Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

Insert

PhyB (PHYB Mustard Weed)

Tags

SIK1 and mCherry

Expression

Yeast

Mutation

1-908aa only

Promoter

ADH1pr

Available Since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pHyg-AID(1-114)-8myc

Plasmid

#99516

Purpose

Truncated C-terminal AID-8myc degron cassette with HygR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(1-116) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

8xMyc

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pEN-R2-mCsfGFP-L3

Plasmid

#178602

Purpose

It can be used to study protein turnover in living plant cells of Arabidopsis (Arabidopsis thaliana) and Nicotiana benthamiana.

Depositor

Frederica Theodoulou

Article

Zhang et al Plant Physiol. 2019 Jun;180(2):718-731.

Insert

Tandem fluorescent protein timers (tFTs)

Use

Entry vector for gateway cloning

Tags

mCherry superfolder GFP

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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PhyB-mCherry-SNF7

Plasmid

#51574

Purpose

Constitutively express endosome localized PhyB in Saccharomyces cerevisiae

Depositor

Chao Tang

Article

Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

Insert

PhyB (PHYB Mustard Weed)

Tags

SNF7 and mCherry

Expression

Yeast

Mutation

1-908aa only

Promoter

ADH1pr

Available Since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pLJM1-CRY2-mCherry-(GGGGS)3-hRGS2box

Plasmid

#112257

Purpose

Expresses RGS component of opto-RGS2 in mammalian cells

Depositor

Brian Chow

Article

Hannanta-Anan et al ACS Synth Biol. 2018 Jun 15;7(6):1488-14

Insert

CRY2-mCherry-(GGGGS)3-hRGS2box

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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CLPIT Cry2PHR-mCh-Rac1

Plasmid

#42955

Depositor

David Schaffer

Article

Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

Insert

Cry2PHR-mCherry-Rac1 (RAC1 Mustard Weed, Human)

Use

Retroviral

Expression

Mammalian

Promoter

Tet-Off promoter

Available Since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

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pLBM0090

Plasmid

#172824

Purpose

Level 2 module containing mCHERRY-nVENUS HSP21-cVENUS

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

[35S-P19][35S-CTPSSU-mHSP21-nVENUS][35S-CTPSSU-mHSP21-X3FLAGcVENUS][35S-SP_SV40-CFP]

Use

Synthetic Biology

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pUPD2 AtUBQ10 (GB2478)

Plasmid

#160594

Purpose

UBQ10 promoter from A. thaliana for constitutive expression in plant systems.

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

Insert

AtUBQ10

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPglu-600uDelta11 Aphrodite

Plasmid

#13569

Depositor

Wolf Frommer

Article

Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

Insert

FLIPglu F16A Delta11 Aphrodite

Tags

Aphrodite and ECFP

Expression

Bacterial

Mutation

Fluorophore-derived linker shortened. F16A mutati…

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
hSTARR-seq_SCP1 vector_blocking 3

Plasmid

#99318

Purpose

Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Type

Empty backbone

Use

Hstarr-seq screening vector

Expression

Mammalian

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGE663

Plasmid

#153261

Purpose

M6E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGE665

Plasmid

#153263

Purpose

M8E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGE654

Plasmid

#153252

Purpose

M1E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
optoPKAW196R/F327A

Plasmid

#104547

Purpose

Expresses Cry2-mCherry-PKA W196R/F327A in mammalian cells.

Depositor

David Lawrence

Article

O'Banion et al Cell Chem Biol. 2017 Oct 25. pii: S2451-

Insert

Cry2-mCherry-PKA W196R/F327A

Tags

mCherry

Expression

Mammalian

Mutation

PKA W196R and Y204A

Promoter

CMV

Available Since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
E1_pENTR_L1-L4_NI_G1397-DddtoxA-C

Plasmid

#171726

Purpose

ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor

Use

Multisite gateway entry vector

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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E3_pENTR_L3-L2_NG_G1397-DddtoxA-C

Plasmid

#171734

Purpose

ptpTALECD vector assembly, Entry clone 1 with attL2 and L3

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor

Use

Multisite gateway entry vector

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
E3_pENTR_L3-L2_NG_G1397-DddtoxA-N

Plasmid

#171735

Purpose

ptpTALECD vector assembly, Entry clone 1 with attL2 and L3

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C- and N termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor

Use

Multisite gateway entry vector

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
E1_pENTR_L1-L4_NI_G1397-DddtoxA-N

Plasmid

#171727

Purpose

ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor

Use

Multisite gateway entry vector

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
E1_pENTR_L1-L4_NN_G1397-DddtoxA-N

Plasmid

#171731

Purpose

ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor

Use

Multisite gateway entry vector

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 3,273 results for: BID;