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Plasmid#203315PurposeOverexpression of BCAM-MCAM chimerasDepositorInserthMUC18/hBCAM
UseTagsFlag (DYKDDDK)ExpressionMammalianMutationIgG V1-C1 of hBCAM fused to Ig like C2-C3 of hMUC…PromoterAvailable sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
hBCAM-B1
Plasmid#203313PurposeOverexpression of BCAM-MCAM chimerasDepositorInserthMUC18/hBCAM
UseTagsFlag (DYKDDDK)ExpressionMammalianMutationIgG V1 of hBCAM fused to Ig like V2-C3 of hMUC18PromoterAvailable sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
hBCAM-M3
Plasmid#203319PurposeOverexpression of BCAM-MCAM chimerasDepositorInserthBCAM/hMUC18
UseTagsFlag (DYKDDDK)ExpressionMammalianMutationIgG V1-C1 of hMuc18 fused to Ig like C2-C3 of hBC…PromoterAvailable sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
hBCAM-B2
Plasmid#203314PurposeOverexpression of BCAM-MCAM chimerasDepositorInserthMUC18/hBCAM
UseTagsFlag (DYKDDDK)ExpressionMammalianMutationIgG V1-V2 of hBCAM fused to Ig like C1-C3 of hMU…PromoterAvailable sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Sha3Cas9
Plasmid#192136PurposeExpresses Sha3Cas9, and cloning backbone for sgRNADepositorInsertSha3Cas9
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsTNRC6Biso1-C-term_F
Plasmid#146301PurposeMammalian Expression of HsTNRC6Biso1-CtermDepositorInsertHsTNRC6Biso1-Cterm (TNRC6B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsTNRC6Biso1-delMI_F
Plasmid#146302PurposeMammalian Expression of HsTNRC6Biso1-delMIDepositorInsertHsTNRC6Biso1-delMI (TNRC6B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsTNRC6Biso1-delMII_F
Plasmid#146303PurposeMammalian Expression of HsTNRC6Biso1-delMIIDepositorInsertHsTNRC6Biso1-delMII (TNRC6B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsTNRC6Biso1-delMI-MII_F
Plasmid#146304PurposeMammalian Expression of HsTNRC6Biso1-delMI-MIIDepositorInsertHsTNRC6Biso1-delMI-MII (TNRC6B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsTNRC6Biso1-N-term_F
Plasmid#146305PurposeMammalian Expression of HsTNRC6Biso1-NtermDepositorInsertHsTNRC6Biso1-Nterm (TNRC6B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-HsTNRC6Biso1_L
Plasmid#146847PurposeInsect Expression of HsTNRC6Biso1DepositorInsertHsTNRC6Biso1 (TNRC6B Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-HsTNRC6Biso1_1219-1621_L
Plasmid#146873PurposeBacterial Expression of HsTNRC6Biso1_1219-1621DepositorInsertHsTNRC6Biso1_1219-1621 (TNRC6B Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
FnRPR9-10
Plasmid#182568PurposeHuman fibronectin fragment, type-III repeats 9-10, R1374A, P1376A, R1379A mutantDepositorInsertHuman fibronectin fragment, type-III repeats 9-10
UseTagsHis and TEV cleavage site after His tagExpressionBacterialMutationR1374A, P1376A, R1379APromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
FnRPR8-10
Plasmid#182570PurposeHuman fibronectin fragment, type-III repeats 8-10, R1374A, P1376A, R1379A mutantDepositorInsertHuman fibronectin fragment, type-III repeats 8-10
UseTagsHis and TEV cleavage site after His tagExpressionBacterialMutationR1374A, P1376A, R1379APromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phat2-dr-cPla2-C2-D34R-D87R-EGFP-6H
Plasmid#187188PurposeProtein ExpressionDepositorInsertENSDARG00000024546 (pla2g4aa Zebrafish)
UseTagsEGFPExpressionBacterialMutationD34R, D87RPromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phat2-dr-cPla2-C2-L32F-L33F-EGFP-6H
Plasmid#187184PurposeProtein ExpressionDepositorInsertENSDARG00000024546 (pla2g4aa Zebrafish)
UseTagsEGFPExpressionBacterialMutationL32F, L33FPromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phat2-dr-cPla2-C2-Y90A-V91A-EGFP-6H
Plasmid#187185PurposeProtein ExpressionDepositorInsertENSDARG00000024546 (pla2g4aa Zebrafish)
UseTagsEGFPExpressionBacterialMutationY90A, V91APromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phat2-dr-cPla2-C2-L32A-L33A-EGFP-6H
Plasmid#187183PurposeProtein ExpressionDepositorInsertENSDARG00000024546 (pla2g4aa Zebrafish)
UseTagsEGFPExpressionBacterialMutationL32A, L33APromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phat2-dr-Alox12-PLAT-EGFP-6H
Plasmid#187190PurposeProtein ExpressionDepositorInsertENSDARG00000069463 (alox12 Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phat2-hs-PKCgamma-C2-EGFP-6H
Plasmid#187115PurposeProtein ExpressionDepositorInsertENSG00000126583 (PRKCG Human)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phat2-dr-cPla2-C2-D34K-D87K-EGFP-6H
Plasmid#187187PurposeProtein ExpressionDepositorInsertENSDARG00000024546 (pla2g4aa Zebrafish)
UseTagsEGFPExpressionBacterialMutationD34K, D87KPromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phat2-dr-cPla2-C2-Y90F-V91F-EGFP-6H
Plasmid#187186PurposeProtein ExpressionDepositorInsertENSDARG00000024546 (pla2g4aa Zebrafish)
UseTagsEGFPExpressionBacterialMutationY90F, V91FPromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUCHR-mClover-MT-C34
Plasmid#177153PurposeLentiviral vector for expression of anti-HIV-1 MT-C34 peptide on cell surfaceDepositorInsertmClover-MT-C34
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRV∆G-NPEST-4Cre
Plasmid#174380PurposeRabies vector for expressing Cre recombinase and N gene with PEST domainDepositorInsertCre recombinase
UseRabiesTagsExpressionMutationPromoterAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJo55
Plasmid#159623PurposeAn empty HIS3 plasmid for translation factor eIF4E complementation test in yeast, strain Jo55. Golden Gate cloning using BsaI. Selection with RFP after cloning.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJo55-CA1
Plasmid#159624PurposePlasmid pJo55, which contains positive control (C. annuum eIF4E-1) for complementation test in Jo55 yeasts.DepositorInsertC. annuum eIF4E-1
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pEarleyGate100-pSpoA-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
Plasmid#140170PurposeExpresses a regulatable secretory cargo for Arabidopsis thalianaDepositorInsertpSpoA-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEarleyGate100-pSpoA-DsRed-Express2-FKBP(LV)(C22V)
Plasmid#140169PurposeExpresses a regulatable secretory cargo (without an ER export signal) for Arabidopsis thalianaDepositorInsertpSpoA-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-pOst1-ESCargo(Orange)
Plasmid#140157PurposeExpresses a regulatable secretory cargo for yeast at the TRP1 locusDepositorInsertpOst1-APVNTT-E2-Orange-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIplac128-pOst1-ESCargo(Orange)
Plasmid#140158PurposeExpresses a regulatable secretory cargo for yeast at the LEU2 locusDepositorInsertpOst1-APVNTT-E2-Orange-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIplac128-pOst1-ESCargo(Crimson)
Plasmid#140156PurposeExpresses a regulatable secretory cargo for yeast at the LEU2 locusDepositorInsertpOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-pOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)
Plasmid#140155PurposeExpresses a regulatable secretory cargo for yeast at the TRP1 locusDepositorInsertpOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRRL-GFPGO2(NGC)-PGK-Neo
Plasmid#136893PurposeLentivirus for base editing activatable GFP expression in mammalian cellsDepositorInsertGFPGO2(NGC)
UseLentiviralTags2xNLSExpressionMutationATG>ACGPromoterSFFVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRRL-GFPGO2(NGT)-PGK-Neo
Plasmid#136892PurposeLentivirus for base editing activatable GFP expression in mammalian cellsDepositorInsertGFPGO2(NGT)
UseLentiviralTags2xNLSExpressionMutationATG>ACGPromoterSFFVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
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BC-A1-016
Plasmid#78704PurposeA weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of GFP under the control of a weak RBS.DepositorInsertBBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0033-GFP-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-015
Plasmid#78703PurposeA weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a weak RBS.DepositorInsertBBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-010
Plasmid#78697PurposeA strong promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of GFP under the control of a strong RBS.DepositorInsertBBa_J23100-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-008
Plasmid#78695PurposeA weak promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a weak RBS.DepositorInsertBBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-007
Plasmid#78694PurposeA weak promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a weak RBS.DepositorInsertBBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-SARA
Plasmid#67389PurposeExpression of native hSara in E. coliDepositorInsertSARA (SAR1B Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV Ub Lex VP16 (F442A) HA (P#1711)
Plasmid#14596DepositorInsertLexA
UseTagsHA, Ubiquitin, and VP16 (F442A)ExpressionMammalianMutationThe VP16 sequence contains an F442A mutation. The…PromoterAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
p075 pCS xTRPS1 delN delC119 (M)
Plasmid#11045DepositorInsertTRPS1 delN delC119 mut (trps1 Frog)
UseXenopus, mammalian, avian, and zebrafishTagsExpressionMutationaa806-1153 of xTRPS1. Two Cys residues in the GA…PromoterAvailable sinceDec. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
p076 pCS xTRPS1 delN delC320 (M)
Plasmid#11046DepositorInsertTRPS1 (trps1 Frog)
UseMammalian, avian, and zebrafishTagsExpressionMutationaa806-952 of xTRPS1. Two Cys residues in the GAT…PromoterAvailable sinceDec. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
p063 pCS xTRPS1 delta N (M)
Plasmid#11036DepositorInsertTRPS1 deltaN mut (trps1 Frog)
UseMammalian, avian, and zebrafishTagsExpressionMutationaa806-1153 of xTRPS1. Two Cys residues in the GA…PromoterAvailable sinceDec. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pILGFPB5A
Plasmid#83556PurposeUsed to evaluate the expression output of GAL1 promoter with yEGFP used as the reporter gene.DepositorInsertGAL1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterGAL1Available sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21a - Ec-ClpX
Plasmid#111516PurposeExpresses E. coli ClpX with a C-terminal His tagDepositorInsertClpX
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL3
Plasmid#48743PurposeExpression of luciferase driven by truncated mPer2 promoter region (bases -1128 to +1060 with respect to transcription start site at +1)DepositorInserttruncated mPer2 promoter/enhancer region (-1128 to +1060)
UseLuciferaseTagsExpressionMutationtruncated promoter region (see pGL6)PromoterAvailable sinceDec. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pL
Plasmid#196286Purpose35S promoter-driven expression of the positive-sense TSWV L RNA segment encoding codon-optimized L (RdRp)DepositorInsertFull length TSWV L antigenome encoding codon-optimized L (RdRp)
UseCRISPRTagsExpressionPlantMutationPromoterduplicated cauliflower mosaic virus 35S promoterAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only