We narrowed to 5,157 results for: PID;
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Plasmid#46946PurposeRetroviral plasmid for co-expressing siRNA-resistant, FKBP-tagged AP-1 gamma subunit and G418 resistance gene in mammalian cellsDepositorInsertAp1g1-FKBP
UseRetroviralTagsMainly mouse Ap1g1 cDNA, with a fragment of brainā¦ExpressionMammalianMutationPromoterAvailable sinceDec. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-P1-N-EGFP-C
Plasmid#159436PurposeCRISPR knock-in donor construct with fluorescent reporterDepositorInsertEGFP
UseCRISPRTags3' linker pair 1, 5' linker pair 1, C tā¦ExpressionMutationPromoterCMVAvailable sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
FFiBlast MSCV Puro
Plasmid#68464PurposeRetroviral plasmid for establishment of blasticidin resistance Cre-reporter cell lineDepositorInsertBlasticidin Resistance
UseCre/Lox and RetroviralTagsExpressionMammalianMutationInvertedPromoterLTRAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pICH-chl-roGFP2-GPXC71S
Plasmid#191703PurposeTi plasmid pICH86988 harbouring roGFP2-GPXC71S -a plastid-targeted control roGFP2 fusion not responsive to peroxides used alongside plasmids 191699 and 191701DepositorInsertchl-redox(ro)GFP2-GPXC71S
UseBinary agrobacterium ti plasmidTagsExpressionPlantMutationPeroxidatic Cysteine 71 replaced with SerinePromoterCaMV 35SAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJBL704
Plasmid#140374PurposeExpresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 4BP dowsntream from the promoterDepositorInsertT7-4BP-tetO-3WJdB-T
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSBL_DK193
Plasmid#156424PurposeInsect cell expression of 3a(Q57H)-sfGFP, needs to be introduced into baculoviral bacmidDepositorInsertSARS-CoV-2 3a protein (Q57H)
UseTagssfGFP-HisExpressionInsectMutationQ57HPromoterPolyhedrinAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pREG(R1-R3) #BGV141
Plasmid#48958PurposeRetroviral Destination vector containing AttR1-AttR3 site. For use with three-way LR reaction with cDNA in entry vector and a marker in pBEG R2-L3 plasmids (module 2 and module 3 plasmids).DepositorTypeEmpty backboneUseRetroviralTagsExpressionMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pXGFPC-5 Pxyl-dcas9 (Spy)
Plasmid#133316Purposeexpresses dcas9 from Streptococcus pyogenes; repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistanceDepositorInsertdcas9 (Streptococcus pyogenes)
UseCRISPRTagsExpressionMutationPromoterxyloseAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
E0030 (YFP)_CD
Plasmid#66031PurposeMoClo Basic Part: CDS - Fluorescent protein. Yellow. [C:E0030:D]DepositorInsertFluorescent reporter - YFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJBL705
Plasmid#140375PurposeExpresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 5BP dowsntream from the promoterDepositorInsertT7-5BP-tetO-3WJdB-T
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pY71-tet.I.8
Plasmid#133521PurposesfGFP driven by a T7 promoter with the tetO sequence 11 bp downstream from the promoter start siteDepositorInsertsfGFP
UseTagsStrep tag and TEV siteExpressionBacterialMutationWTPromoterT7Available sinceDec. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-24
Plasmid#172750PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-24; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-24
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-9
Plasmid#172742PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-9; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-9
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBEG R2-iCreT2ALuc-L3 #BGV208
Plasmid#48992PurposeSupplies Cre recombinase and Luciferase and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).DepositorInsertscre recombinase
Luciferase
UseLuciferase; Gateway cloning vectorTagsIRES (weak)ExpressionMutationPromoterUnknownAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGK:HygroR-CMV:mKO2-C_UTR
Plasmid#68481PurposeLentivirus expressing mKO2 with UTR sensor cassette and hygromycin resistanceDepositorInsertsHygromycin Resistance
mKO2-C_UTR
UseLentiviralTagsExpressionMammalianMutationshRNA sensor cassette "B" added to 3ā¦PromoterCMV and pGKAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCheck2 Dest (R1-R2) #BGV129
Plasmid#48955PurposeDestination dual luciferase reporter plasmid for checking effectiveness of shRNA. Compatible with standard AttL1-AttL2 Gateway Entry vectors.DepositorTypeEmpty backboneUseLuciferase; Gateway cloning vectorTagsExpressionMutationPromoterSV40Available sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
CTA2-mNG211
Plasmid#141167PurposeExpresses GST-SUMO tagged CTA2 Subunit with 11th StrandDepositorInsertSUMO-CTA2-mNG211
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA pDEST40 Cas9 GW STOP
Plasmid#183196PurposeDestination vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-8
Plasmid#172761PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-8; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-8
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-5
Plasmid#172760PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-5; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-5
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
secCitrine
Plasmid#181964PurposeBasic chitinase signal peptide fused to Citrine, under control of MUM4_1.5kb promoterDepositorInsertsignal peptide sequence from basic chitinase
UseTagsCitrine YFPExpressionPlantMutationPromoter1.5 kb MUM4 promoterAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
MTK3_001
Plasmid#123952PurposeEncodes nuclear localized PHR domain of CRY2 fused to activating domain of p65 as Type 3 part to be used in the MTK systemDepositorInsert3XNLS::CRY2PHR::p65
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAGGs-Hoxc8
Plasmid#45555DepositorInsertHoxc8 (HOXC8 Chicken)
UseTagsExpressionMutationPromoterCMV / b-actinAvailable sinceAug. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 Cas9-HMGB1
Plasmid#183198PurposeEntry cloneDepositorInsertCas9-HMGB1
UseCRISPR; Gateway entry cloneTagsExpressionMutationn/aPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-6
Plasmid#163132PurposepLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PelB signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 Cas9-RFC4
Plasmid#183199PurposeEntry cloneDepositorInsertCas9-RFC4
UseCRISPR; Gateway entry cloneTagsExpressionMutationn/aPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p15a-PLtetO-1-UTR1-LacI-T500
Plasmid#45784DepositorInsertLac repressor
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLtetO-1Available sinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR U6-miR-30 R4-R3
Plasmid#62117PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseRNAi; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSCIP-hTRAC
Plasmid#154094PurposeSelf-Cutting and Integrating CRISPR backbone targeting human TCR-alpha common chainDepositorInsertsgRNA and BAIT targeting human TRAC locus
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
GST-TEVcs-ATG101
Plasmid#171414PurposeFor the purifcation of human ULK1 complexDepositorInsertATG101 (ATG101 Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pK RagA-RagC
Plasmid#99649PurposeBacterial expression of wildtype Rag GTPase dimerDepositorUseTags10x His tag, Poly arginine linker, and SUMOExpressionBacterialMutationPromoterT7Available sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR CMV L5-L2
Plasmid#62091PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and multiple cloning site module. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseMule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
gRD21A-mRFP
Plasmid#181959PurposeRD21A protein tagged with mRFP, under control of native promoterDepositorInsertRD21A genomic sequence including 2kb upstream of the ATG
UseTagsmRFPExpressionPlantMutationPromoterRD21A nativeAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Kp-2
Plasmid#163144PurposepLyGo cloning vector for a sequence of interest (LPMO) in K. phaffii. Vector encoding the alfa-amylase signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAOX1Available sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS1088
Plasmid#80159PurposepET-Duet for bacterial expression of Cys-NuG2(4x)-Ald and co-expression of Mycobacterium tuberculosis Formylglycine Generating EnzymeDepositorInsertHisSUMO-Cys-4xNuG2-Ald
UseTagsHisSUMOExpressionMutationPromoterT7Available sinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
MTK0_012
Plasmid#123967PurposeEncodes the hROSA26 BxBI recognition site with Hygromycin resistance and mRuby2 landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK systemDepositorInserthRosa26-BxBI landing pad
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
p3E-OSBP
Plasmid#67677PurposeMultisite gateway entry clone for adding C-terminal fusions of OSBPDepositorInsertOSBP
UseGateway multisite 3' entry cloneTagsExpressionMutationaa 87-185 of NP_002547.1PromoterAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-2
Plasmid#172739PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-2; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-2
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
GST-TEVcs-GFP-ATG101
Plasmid#171415PurposeFor the purifcation of GFP-ULK1 complexDepositorInsertATG101 (ATG101 Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
GST-GFP-ATG14
Plasmid#171426PurposeFor the purifcation of GFP- PI3KC3 complexDepositorInsertATG14 (ATG14 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
TB:2xP53_RE:MiniP
Plasmid#133881Purpose2 copies of the p53 DNA binding motifDepositorInsert2 copies of the p53 DNA binding motif
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry-SAC1-HLx8
Plasmid#108139PurposeSAC1 with extended linkerDepositorInsertmCherry:SACM1L(1-520):[EAAAR]8:SACM1L(521-587) (SACM1L Human)
UseTagsExpressionMammalianMutationSACM1L(1-520):[EAAAR]8:SACM1L(521-587)PromoterCMVAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETDuet RagA-RagC(L91P)
Plasmid#99647PurposeBacterial expression of RagA-RagC(L91P)DepositorUseTagsHis tagExpressionBacterialMutationL91PPromoterAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
JPF0522
Plasmid#124013PurposeEncodes crippled pEF1a driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vectorDepositorInsertPB_pEF1ac-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
JPF0454
Plasmid#124039PurposeEncodes pPGK1 driving expression of spCas9 P2A mAzamiGreen in a 2nd gen. Lenti Virus destination vectorDepositorInsertpHR_pPGK1-spCAS9-P2A-mAzamiGreen
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMDC84-SEIPIN3-Ndelta
Plasmid#96981PurposeExpress N-terminal truncated Arabidopsis SEIPIN3 gene in plants.DepositorInsertSEIPIN3 (AT2G34380 Mustard Weed)
UseTags6xHis and GFPExpressionPlantMutationA segment of 507 nucleotides are deleted from theā¦Promoter35SAvailable sinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSBL_DK198
Plasmid#156425PurposeInsect cell expression of 3a delta N(aa 42-275)-sfGFP, needs to be introduced into baculoviral bacmidDepositorInsertSARS-CoV-2 3a protein (aa 42-275)
UseTagssfGFP-HisExpressionInsectMutationaa 42-275PromoterPolyhedrinAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK234_027
Plasmid#123907PurposeEncodes the spCas9 sgRNA1 hThal5.10-2_hg18 (site 1) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA1 -hThal5.10-2_hg18
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
DVA_AG
Plasmid#66042PurposeMoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [A:LacZa:G]DepositorTypeEmpty backboneUseSynthetic Biology; Destination vectorTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
MTK0_001
Plasmid#123924PurposeEncodes the spCas9 gRNA GFP dropout expression cassette with ConLS and ConRE connectors with Ampicillin resistance as a type 0 part to be used in the MTK systemDepositorInsertTU-sgRNA - L1/RE
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only