We narrowed to 2,572 results for: GEM
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Plasmid#68185PurposeProvides the Gal4 Binding Domain fused to the BDR Transcriptional Repressor as a level 0 GoldenBraid partDepositorInsertGal4 Binding Domain fused to BDR Transcriptional Repressor
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBOB-EF1-FastFUCCI-Puro
Plasmid#86849Purpose3rd generation lentiviral vector encoding FUCCI reporters for cell cycle analysis and puro for selectionDepositorInsertsmKO2-hCDT1(30-120)
mAG-hGEM(1-110)
pac (puromycin N-acetyl-transferase)
UseLentiviralExpressionMammalianPromoterEF1 and PGKAvailable SinceNov. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-HOT-P2A-Clover-BlastR
Plasmid#138568PurposeUniversal Cleavable Donor Plasmid for tagging with P2A-Clover-BlastR using NHEJDepositorInsertP2A-clover-BlastR
UseCRISPRAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUPD P35S (GB0552)
Plasmid#160554Purpose35s promoter for N-terminal fusionsDepositorInsert35s
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJC5-4-insulator-BREreporter-insulator
Plasmid#171499Purposeinjections to make Bmp reporter mouse transgenic lineDepositorInsertInsulator BRE-MLP:H2B-Turqoise
ExpressionMammalianPromoterBRE-MLPAvailable SinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pISH-Ascl1
Plasmid#74338PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Ascl1 (also known as Mash1). Not for gene expression.DepositorInsertachaete-scute family bHLH transcription factor 1 (Ascl1 Mouse)
Available SinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMGF157
Plasmid#96972Purposeish1-mCherry insertion cassette with Hygro resistance geneDepositorInsertish1 (ish1 Fission Yeast)
TagsGST and mCherryExpressionBacterialMutationish1 C-terminal domain fused with mCherry + hygro…PromoterN/AAvailable SinceAug. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMGF170
Plasmid#96997Purposeish1-mCherry insertion cassette with NAT resistance geneDepositorInsertish1 (ish1 Fission Yeast)
TagsGST and mCherryExpressionBacterialMutationish1 C-terminal domain fused with mCherry + NAT r…PromoterN/AAvailable SinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHIV-NAT-T2A-hCD52-eGFP-NFATC2IP-SPR
Plasmid#221447PurposeLentiviral mammalian expression vector of eGFP-tagged NFATC2IP ORF that is fused with S-phase restricted (SPR) degron at its C-terminusDepositorInsertNFATC2IP (NFATC2IP Human)
UseLentiviralTagseGFPExpressionMammalianMutationSPR degron: hGEM (1-110) fragment that is fused t…PromoterCMVAvailable SinceJuly 3, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMGF130
Plasmid#96971PurposeS. pombe lem2 knockout cassette with hygro resistance geneDepositorInsertlem2 (lem2 Fission Yeast)
ExpressionBacterialMutationwhole ORF deletion and replaced with hygro resist…PromoterN/AAvailable SinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMGF169
Plasmid#96996Purposeish1 mCherry insertion cassette with G418 resistance geneDepositorInsertish1 (ish1 Fission Yeast)
TagsGST and mCherryExpressionBacterialMutationish1 C-terminal domain fused with mCherry + G418 …PromoterN/AAvailable SinceAug. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRN120
Plasmid#160696PurposeBeYDV viral replicon on T-DNA backbone expressing Firefly Luc+, CmYLCV::STM::35S terminator, Nos::WUS2::PinII terminatorDepositorInsertsCmYLCV::Luc+::AtHSP
Nos::WUS2::PinII
AtUbi10::STM::35S
UseLuciferase; Gemini viral replicon, plant developm…PromoterAtUbi10, CmYLCV, and NosAvailable SinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-3*Flag-APEX2 C-term SMN2
Plasmid#187587PurposeExpress FLAG epitope and APEX2-tagged SMN2 fusion protein in mammalian cellsDepositorAvailable SinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pME-FastFUCCI (JDW 1488)
Plasmid#242556PurposeGateway middle entry clone containing FastFUCCI reporter; For visualizing cell cycle stateDepositorInsertmKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)
UseGateway subcloningAvailable SinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
Gfp-rt-vasa
Plasmid#167322PurposePlasmid for synthesis of GFP chimeric RNA that contained vasa-3'UTRs of rainbow trout by in vitro transcription.DepositorInsertrt vasa 3'UTRs (ddx4 rainbow trout)
ExpressionBacterialAvailable SinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
tRNA-gRNA position [D1_n-1] (GB1208)
Plasmid#75409PurposetRNA and scaffold for the assembly of GBoligomers for the first position (positon [D1_n-1]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter (2-part multiplexing)DepositorInserttRNA-gRNA position [D1_n-1]
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
SMN1-1
Plasmid#156022PurposeFor use in RBP tethering screenDepositorAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
tRNA-gRNA position [n] (GB1207)
Plasmid#75408PurposetRNA and scaffold for the assembly of GBoligomers for the last position (positon [n]) of a polycistronic tRNA-gRNA (2- and 3-part multiplexing)DepositorInserttRNA-gRNA position [n]
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV Intein-C-SpyCas9, CMV-AcrIIA4-scaffold (2xBsmBI sites)
Plasmid#120294PurposeAAV Vector for expression of C-terminal SpyCas9 fragemnt with split-intein and a CMV-driven AcrIIA4 (no miR binding sites)DepositorInsertC-terminal fragment of SpyCas9
UseAAV and CRISPRTagssplit-inteinExpressionMammalianAvailable SinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
tRNA-gRNA position [2_n-1] (GB1206)
Plasmid#75407PurposetRNA and scaffold for the assembly of GBoligomers for the intermediate position (positon [2_n-1]) of a polycistronic tRNA-gRNA (3-part multiplexing)DepositorInserttRNA-gRNA position [2_n-1]
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
tRNA-gRNA position [D1_2] (GB1205)
Plasmid#75406PurposetRNA and scaffold for the assembly of GBoligomers for the first position (positon [D1_2]) of a polycistronic tRNA-gRNA regulated by the dicot U6-26 or U6-1 promoter (3-part multiplexing)DepositorInserttRNA-gRNA
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pD10AH840AhCas9 (GB1041)
Plasmid#68207PurposeProvides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid partDepositorInsertCas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
UseCRISPR and Synthetic BiologyMutationBsaI and BsmBI sites removed; human codon optimis…Available SinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN-FastFUCCI (JDW 1494)
Plasmid#242592PurposeA CAGGS driven expression vector containing FastFUCCI to label cell cycle state.DepositorInsertmKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)
ExpressionMammalianPromoterCAGGSAvailable SinceDec. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME-zFUCCI (JDW 1464)
Plasmid#242558PurposeGateway middle entry clone containing zebrafish FUCCI reporter; For visualizing cell cycle state.DepositorInsertmCerulean-zGeminin(1-100) P2A mCherry-zCdt1(1-190)
UseGateway subcloningAvailable SinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUPD 5'UTR:YFP:Tnos (GB1483)
Plasmid#160557PurposePart 3/3 for assembly of site-specific recombination based Registers. Composed of the 5'UTR sequence of 35S promoter, coding sequence of YFP and NOS terminator (5'UTR:YFP:TNos)DepositorInsert5'UTR:YFP:Tnos
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVD1 Multiplexing Edit E3-E4-En-1 (GB2244)
Plasmid#160566PurposeGBoligomers for the position [3_(n-1)] of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.DepositorInsertMultiplexing Edit (E3-E4-En-1)
UseCRISPR and Synthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
Gfp-zf-vasa
Plasmid#167323PurposePlasmid for synthesis of GFP chimeric RNA that contained vasa-3'UTRs of zebrafish by in vitro transcription.DepositorInsertzf vasa 3'UTRs (ddx4 Zebrafish)
ExpressionBacterialAvailable SinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVD1 M1_1-pTRNA-scf 2.1 (GB2603)
Plasmid#160568PurposetRNA and scaffold 2.1 scRNA aptamer Ms2 for the assembly of GBoligomers for a single position of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.DepositorInsertM1_1-pTRNA-scf 2.1
UseCRISPRMutationBsaI and BsmBI sites removedAvailable SinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
tRNA-gRNA position [M1_n-1] (GB1210)
Plasmid#75411PurposetRNA and scaffold for the assembly of GBoligomers for the first position (positon [M1_n-1]) of a polycistronic tRNA-gRNA regulated by the (monocot) U3 promoter (2-part multiplexing)DepositorInserttRNA-gRNA position [M1_n-1]
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
CmCitrine Lck w/ NosUTRs
Plasmid#66785Purposeexpression of fluorescent membranes in sea urchin primordial germ cellsDepositorInsertLck (LCK Human)
UseSea urchin, zebrafish, xenopusTags3' UTR from sea urchin Nanos, 5' UTR fr…MutationTandem repeat of LCK the N-terminal membrane targ…PromoterT7Available SinceJuly 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVD1 Multiplexing Edit E4 (GB2241)
Plasmid#160563PurposetRNA and scaffold for the assembly of GBoligomers for position [4-5] of a polycistronic tRNA-gRNA.DepositorInsertMultiplexing Edit (E4)
UseCRISPR and Synthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTol2-Fli1-zFUCCI-IRES-mTagBFP2 (JDW 1517)
Plasmid#242602PurposeA Tol2 expression vector containing the fli promoter driving FUCCI to label cell cycle in all blood vessels followed by an IRES-mTagBFP2.DepositorInsertmCerulean-zGem-P2A-mCherry-zCdt1, nls-mTagBFP2
UseZebrafish expressionPromoterfli promoterAvailable SinceDec. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN-FastFUCCI-IRES-myr-mKate2 (JDW 1503)
Plasmid#242598PurposeA CAGGS driven expression vector containing FastFUCCI to label cell cycle state followed by an IRES and a membrane targeted mKate2DepositorInsertmKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)
ExpressionMammalianPromoterCAGGSAvailable SinceDec. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN-FastFUCCI-IRES-myr-mTagBFP (JDW 1502)
Plasmid#242597PurposeA CAGGS driven expression vector containing FastFUCCI to label cell cycle state followed by an IRES and a membrane targeted mTagBFP2DepositorInsertmKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)
ExpressionMammalianPromoterCAGGSAvailable SinceDec. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME-zFUCCI-P2A-H2A-tdiRFP (JDW 1380)
Plasmid#242559PurposeGateway middle entry clone containing a zebrafish FUCCI reporter followed by H2A tdiRFP reporter; For visualizing cell cycle state.DepositorInsertmCerulean-zGeminin(1-100) P2A mCherry-zCdt1(1-190)
UseGateway subcloningAvailable SinceSept. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVD1 M3_3-pTRNA-scf 2.1 (GB2075)
Plasmid#160560PurposetRNA and scaffold 2.1scRNA aptamer Ms2 for the assembly of GBoligomers for the first position (positon [M3_3]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoterDepositorInsertM3_3-pTRNA-scf 2.1
UseCRISPRMutationBsaI and BsmBI sites removedAvailable SinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVD1 M1_3-pTRNA-scf 2.1 (GB2073)
Plasmid#160558PurposetRNA and scaffold 2.1scRNA aptamer Ms2 for the assembly of GBoligomers for the first position (positon [M1_3]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.DepositorInsertM1_3-pTRNA-scf 2.1
UseCRISPRMutationBsaI and BsmBI sites removedAvailable SinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVD1 M2_3-pTRNA-scf 2.1 (GB2074)
Plasmid#160559PurposetRNA and scaffold 2.1scRNA aptamer Ms2 for the assembly of GBoligomers for the first position (positon [M2_3]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoterDepositorInsertM2_3-pTRNA-scf 2.1
UseCRISPRMutationBsaI and BsmBI sites removedAvailable SinceFeb. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV Intein-C-SpyCas9, CMV-AcrIIA4-2xmiR-122 target sites
Plasmid#120295PurposeAAV Vector for expression of C-terminal SpyCas9 fragement with split-intein and a CMV-driven AcrIIA4 with two miR-122 binding sitesDepositorInsertC-terminal fragment of SpyCas9
UseAAV and CRISPRTagssplit-inteinExpressionMammalianAvailable SinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
tRNA-gRNA position [M1_2] (GB1209)
Plasmid#75410PurposetRNA and scaffold for the assembly of GBoligomers for the first position (positon [M1_2]) of a polycistronic tRNA-gRNA regulated by the (monocot) U3 promoter (3-part multiplexing)DepositorInserttRNA-gRNA position [M1_2]
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only