We narrowed to 2,463 results for: T2A

1. linker-T2A-NTRv2-linker-P2A-mNeonGreen-CAAX ORF2

   Plasmid

   #241193

   Purpose

   Middle entry vector for mini-Golden subcloning platform; NTRv2; mNeonGreen

   Depositor

   Junsu Kang

   Insert

   NTRv2; mNeonGreen
   

   Use

   Mini-golden

   Mutation

   TCGC linker

   Available Since

   Aug. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. linker-T2A-NTRv2-linker-P2A-mNeonGreen-CAAX ORF1

   Plasmid

   #241192

   Purpose

   Middle entry vector for mini-Golden subcloning platform; NTRv2; mNeonGreen

   Depositor

   Junsu Kang

   Insert

   NTRv2; mNeonGreen
   

   Use

   Mini-golden

   Mutation

   TCGC linker

   Available Since

   Aug. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRCA903 - KLF5 isoform 1 Puro-T2A-BFP (pRCA845 backbone)

   Plasmid

   #238184

   Purpose

   Lentiviral vector for overexpression KLF5 ORF from a EF1a promoter

   Depositor

   Thomas Norman

   Article

   Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

   Insert

   KLF5 (KLF5 Human, Synthetic)
   

   Use

   Lentiviral

   Tags

   mTagBFP2

   Available Since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-flx-fDIO-HA-Rpl10a-T2A-myc-hM3D(Gq)

   Plasmid

   #223654

   Purpose

   Expresses vTRAP and hM3D(Gq) in a FlpON-CreOFF dependent manner

   Depositor

   Oscar Marin

   Article

   Selten et al Nature. 2025 Apr 30. doi: 10.1038/s41586

   Insert

   vTRAP-T2A-hM3D(Gq)
   

   Use

   AAV

   Tags

   HA

   Promoter

   hSyn

   Available Since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. LSM0258 (PB)EF1a-ADAR2dd-PCP-T2A-PuroR-P2A-BFP

   Plasmid

   #233446

   Purpose

   PiggyBac donor plasmid to overexpress ADAR2dd-PCP

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   ADAR2dd-PCP-T2A-PuroR-P2A-BFP
   

   Expression

   Mammalian

   Mutation

   E488Q, T501A on ADAR2dd

   Promoter

   EF1a

   Available Since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. LSM0253 (PB)EF1a-ADAR2dd-MCP-T2A-PuroR-P2A-BFP

   Plasmid

   #233444

   Purpose

   PiggyBac donor plasmid to overexpress ADAR2dd-MCP

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   ADAR2dd-MCP-T2A-PuroR-P2A-BFP
   

   Expression

   Mammalian

   Mutation

   E488Q, T501A on ADAR2dd

   Promoter

   EF1a

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV hU6-sgKRAS hUbC-dCas9-KRAB-T2a-GFP2

   Plasmid

   #184557

   Purpose

   Knockdown of murine KRAS gene by targeting the promoter region via CRISPRi system

   Depositor

   Sandeep Prabhu

   Article

   Qian et al Cell Rep. 2023 Jul 25;42(7):112794. doi:

   Insert

   Kirsten rat sarcoma virus (Kras Mouse)
   

   Use

   CRISPR, Lentiviral, and Mouse Targeting

   Tags

   Flag

   Expression

   Bacterial

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pME-3xHA-BirA-T2A-mCherry-caax (JDW 889)

   Plasmid

   #224512

   Purpose

   A Gateway compatible middle entry clone containing Biotin Ligase followed by T2A and mCherry membrane reporter (biotinylation and flourescent cell membrane reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   3xHA-BirA-T2A-mCherry-Caax
   

   Use

   Gateway cloning

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAVS1-Puro cTnT CCND2-T2A-ChloC-P2A-eGFP

   Plasmid

   #179840

   Purpose

   donor plasmid for cardiac-specific CCND2-T2A-Chloc-P2A-eGFP expression in human cells

   Depositor

   Xiaoping Bao

   Article

   Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

   Insert

   CCND2 (CCND2 Human)
   

   Use

   CRISPR and TALEN; Donor plasmid

   Tags

   chloride-conducting ChR (ChloC) and eGFP

   Expression

   Mammalian

   Promoter

   Troponin T promoter

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti gRNA KO OCLNx2 spCas9-T2A-iRFP670-P2A-puro

    Plasmid

    #208399

    Purpose

    codes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, iRFP670 fluorescent protein and puromycin resistance

    Depositor

    Raphael Gaudin

    Article

    Baptiste Pradel et al bioRxiv

    Insert

    hU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    iRFP670

    Expression

    Mammalian

    Available Since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti gRNA KO OCLNx2 spCas9-T2A-Crimson-P2A-puro

    Plasmid

    #208398

    Purpose

    codes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, E2-Crimson fluorescent protein and puromycin resistance

    Depositor

    Raphael Gaudin

    Article

    Baptiste Pradel et al bioRxiv

    Insert

    hU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    E2-Crimson

    Expression

    Mammalian

    Available Since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti gRNA KO OCLNx2 spCas9-T2A-mNeonGreen-P2A-puro

    Plasmid

    #208400

    Purpose

    codes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, mNeonGreen fluorescent protein and puromycin resistance

    Depositor

    Raphael Gaudin

    Article

    Baptiste Pradel et al bioRxiv

    Insert

    hU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Available Since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAR015 Lenti pEF-dEnAsCas12a-NLS-NFZ-3XHA-T2A-BSD

    Plasmid

    #195544

    Purpose

    dCas12a effector fusion for manipulating transcription

    Depositor

    Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Lenti pEF-dEnAsCas12a-NLS-NFZ-3XHA-T2A-BSD
    

    Use

    CRISPR and Lentiviral

    Tags

    3XHA

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMini-CMV-NLS-dead R-IscB (D60A, H269A)-NLS_T2A_mCherry_U6-ωRNA

    Plasmid

    #246430

    Purpose

    All-in-one plasmid. Expresses R-IscB in mammalian cells. Dead mutations included.

    Depositor

    Ailong Ke

    Article

    Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.

    Inserts

    O.gue IscB with dead mutations (D60A, H269A) and delta-TID
    mCherry
    ωRNA
    

    Tags

    SV40 NLS, Thosea asigna virus 2A peptide, and nuc…

    Expression

    Mammalian

    Mutation

    changed Aspartic Acid 60 to Alanine, changed Hist…

    Promoter

    CMV and U6

    Available Since

    Oct. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMini-CMV-NLS-dead R-IscB-NLS_T2A_EGFP (Y67X)_LNMA intron

    Plasmid

    #246435

    Purpose

    Expresses inactive EGFP (Y67X) in mammalian cells for trans-splicing assessments. LMNA intron is inserted into EGFP CDS.

    Depositor

    Ailong Ke

    Article

    Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.

    Inserts

    O.gue IscB with dead mutations (D60A, H269A) and delta-TID
    EGFP
    

    Tags

    SV40 NLS, Thosea asigna virus 2A peptide, and nuc…

    Expression

    Mammalian

    Mutation

    changed Aspartic Acid 60 to Alanine, changed Hist…

    Promoter

    CMV

    Available Since

    Oct. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAR013 Lenti pEF-dEnAsCas12a-NLS-VP64-3XHA-T2A-BSD

    Plasmid

    #195547

    Purpose

    dCas12a effector fusion for manipulating transcription

    Depositor

    Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Lenti pEF-dEnAsCas12a-NLS-VP64-3XHA-T2A-BSD
    

    Use

    CRISPR and Lentiviral

    Tags

    3XHA

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSLQ8702 pHR-TRE3G-AcrVA1-EF1a-sfGFP-T2A-rtta-WPRE

    Plasmid

    #140229

    Purpose

    Dox-inducible expression of anti-Cpf1 AcrVA1. Constitutive EF1a driven rtTA and GFP

    Depositor

    Stanley Qi

    Article

    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

    Insert

    AntiCRISPR protein AcrVA1
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLG1 library vector (pU6-sgRNA Ef1alpha-Puro-T2A-mcherry)

    Plasmid

    #217306

    Purpose

    sgRNA mcherry + Puromycin

    Depositor

    Luke Gilbert

    Insert

    pU6-sgRNA, Ef1alpha-Puro-T2A-mcherry
    

    Use

    CRISPR

    Promoter

    U6, Ef1alpha

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. MSCV-pU6-(BbsI)-CcdB-(BbsI)-Pgk-Puro-T2A-BFP

    Plasmid

    #86457

    Purpose

    Mouse stem cell retroviral vector including a puromycin resistance and BFP gene. sgRNA targets can be cloned in between the BbsI sites

    Depositor

    Ralf Kuehn

    Article

    Chu et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

    Insert

    BFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Pgk

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. PB-TRE-dCas9-VPR-pA-3xsgRNA-EF1a-Tet.O-T2A-PuroR-polyA

    Plasmid

    #166693

    Purpose

    Express dCas9-VPR in a doxycyclin-inducable system and 3 sgRNAs targeting the murine Cnga1 promoter. Can be stably integrated into the genome via the PiggyBac Transposon system.

    Depositor

    Elvir Becirovic

    Article

    Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

    Inserts

    dCas9-VPR
    3x Cnga1 promoter-targeting sgRNAs
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    TRE and U6

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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