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Plasmid#128680Purposemammalian expression of Flag-Polß(TM-K244A: L301R/V303R/V306R-K244A) portein with puromycin selectionDepositorInsertPolB (POLB Human)
UseLentiviralTagsFlagExpressionMammalianMutationL301R/V303R/V306R/K244APromoterCMVAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-Polß(TM)-IRES-neo
Plasmid#128671Purposemammalian expression of Flag-Polß(TM: L301R/V303R/V306R) protein with neomycin selectionDepositorInsertPolB (POLB Human)
UseLentiviralTagsFlagExpressionMammalianMutationL301R/V303R/V306RPromoterCMVAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV HA hRb delta CDK
Plasmid#58906Purposemammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removedDepositorInsertRb (RB1 Human)
UseTagsHAExpressionMammalianMutationall 15 Ser/Thr Cdk acceptor sites changed to Ala,…PromoterCMVAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo:Beta2meGFP
Plasmid#45096DepositorInsertnAChR beta2 (Chrnb2 Mouse)
UseTagsExpressionMammalianMutationThere are strain differences between C57BJ/6 and …PromoterCMVAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
DOX_SAM_CRISPR-activation-2A-GFP-AAVS1-targeting-construct
Plasmid#176836PurposeTargeting construct for intergrating a doxycycline (DOX) inducible SAM CRISPR activation complex -2A-GFP to the AAVS1 locus.DepositorInsertdCas9-VP64-2A-MS2-p65-HSF1-2A-GFP
UseUnspecifiedTagsExpressionMutationSee Depositor Comments BelowPromoterTet operator (tetracycline inducible expression)Available sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
eGFP-proα2(I)
Plasmid#119826PurposeExpresses mouse Type I procollagen α2 chain (Col1a2) with GFP between signal sequence and exon 6DepositorInsertType I procollagen α2 chain (Col1a2 Mouse)
UseTagseGFPExpressionMammalianMutationCol1a2 exons 2-5 replaced by fluorescent tagPromoterCMVAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCerulean-proα1(I)
Plasmid#119842PurposeExpresses mouse Type I procollagen α1 chain (Col1a1) with mCerulean replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage siteDepositorInsertType I procollagen α1 chain (Col1a1 Mouse)
UseTagsmCeruleanExpressionMammalianMutationCol1a1 exons 2-3 replaced by fluorescent tagPromoterCMVAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
eGFP-proα1(I)
Plasmid#119843PurposeExpresses mouse Type I procollagen α1 chain (Col1a1) with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage siteDepositorInsertType I procollagen α1 chain (Col1a1 Mouse)
UseTagseGFPExpressionMammalianMutationCol1a1 exons 2-3 replaced by fluorescent tagPromoterCMVAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
BII-BCA-Prom-ASCL2
Plasmid#133394PurposePiggybac vector for heterologous promoter reporter assay of human ASCL2 promoterDepositorInsertdestabilized tdTomato-NLS and destabilized NLS-GFP
UseTagsExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free) drives expressi…Available sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIRESh-GFPII-SM-Dlk1
Plasmid#108932PurposeGFP-tagged vector expressing full length isoform of Dlk1DepositorInsertDlk1 (Dlk1 Mouse)
UseTagsGFPIIExpressionMammalianMutationPromoterAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-ALDH1A3
Plasmid#189750PurposeThe construct was used to express recombinant ALDH1A3 in E.Coli. The purified protein was used for activity assay.DepositorInsertALDH1A3 (ALDH1A3 Human)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
eGFP-proα2(I)-L
Plasmid#120160PurposeExpresses mouse Type I procollagen α2 chain (Col1a2) with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage siteDepositorInsertType I procollagen α2 chain (Col1a2 Mouse)
UseTagseGFPExpressionMammalianMutationCol1a2 exons 2-3 replaced by fluorescent tagPromoterCMVAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE GFP-Ago2 S387A
Plasmid#60654PurposeGFP-Ago2 S387A mutant lentiviral vectorDepositorInsertArgonaute 2 (AGO2 Human)
UseLentiviralTagsGFPExpressionMutationS387APromoterEF1aAvailable sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
mApple-proα2(I)-L
Plasmid#120161PurposeExpresses mouse Type I procollagen α2 chain (Col1a2) with mApple replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage siteDepositorInsertType I procollagen α2 chain (Col1a2 Mouse)
UseTagsmAppleExpressionMammalianMutationCol1a2 exons 2-3 replaced by fluorescent tagPromoterCMVAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.2/V5-DEST CDC42EP1 WT
Plasmid#69819PurposeExpresses CDC42EP1-V5 in mammalian cellsDepositorInsertCDC42 effector protein (CDC42EP1 Human)
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-RNF168 delta RING
Plasmid#133982Purposemammalian expression vector of Flag tagged RNF168 where the N-terminus of RNF168 has been deleted (including RING domain)DepositorInsertRNF168 (RNF168 Human)
UseTagsFlagExpressionMammalianMutationdeletion of RINGPromoterCMVAvailable sinceNov. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pENTR1A-HA-Jag1
Plasmid#46052DepositorInsertJag1 (Jag1 Rat)
UseEntry vectorTagsHA tagExpressionMutationPromoterAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Sec22b-P33-shR
Plasmid#208359PurposeMammalian expression of fluorescent N-terminally tagged shRNA resistant Sec22b-P33 mutantDepositorInsertSec22b (Sec22b Rat)
UseTagsEGFPExpressionMammalianMutationfour silent mutations (shRNA resistance), 33 aa p…PromoterCMVAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-ALDH1A1
Plasmid#189749PurposeThe construct was used to express recombinant ALDH1A1 in E.Coli. The purified protein was used for activity assay.DepositorInsertALDH1A1 (ALDH1A1 Human)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
mApple-proα1(I)
Plasmid#119844PurposeExpresses mouse Type I procollagen α1 chain (Col1a1) with mApple replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage siteDepositorInsertType I procollagen α1 chain (Col1a1 Mouse)
UseTagsmAppleExpressionMammalianMutationCol1a1 exons 2-3 replaced by fluorescent tagPromoterCMVAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX 4T-1 GST-Ago2 S387A
Plasmid#60656PurposeExpresses GST-Ago2 S387A mutant in mammalian cellsDepositorInsertArgonaute 2 (AGO2 Human)
UseTagsGSTExpressionBacterialMutationS387APromotertacAvailable sinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBpuro_KLF4_ER
Plasmid#34609DepositorInsertKLF4 (KLF4 Human)
UseRetroviralTagsMyc epitope tag and estrogen receptor fusionExpressionMammalianMutationPromoterLTRAvailable sinceJan. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-RNF168 delta MIU1
Plasmid#133978Purposemammalian expression vector of Flag tagged RNF168 where the first motif interacting with ubiquitin (MIU) has been deletedDepositorInsertRNF168 (RNF168 Human)
UseTagsFlagExpressionMammalianMutationdeletion of MIU1PromoterCMVAvailable sinceNov. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA4-His-Max-A-PQBP1-Y65C
Plasmid#25803DepositorInsertPQBP1 with Y65C mutation (PQBP1 Human)
UseTagsHis and XpressExpressionMammalianMutationTyrosine 65 within the WW domain of PQBP1 is chan…PromoterAvailable sinceJuly 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
mApple-proα2(I)
Plasmid#119840PurposeExpresses mouse Type I procollagen α2 chain (Col1a2) with mApple between signal sequence and exon 6DepositorInsertType I procollagen α2 chain (Col1a2 Mouse)
UseTagsmAppleExpressionMammalianMutationCol1a2 exons 2-5 replaced by fluorescent tagPromoterCMVAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-RNF168 delta MIU1/MIU2
Plasmid#133981Purposemammalian expression vector of Flag tagged RNF168 where both motifs interacting with ubiquitin have been deletedDepositorInsertRNF168 (RNF168 Human)
UseTagsFlagExpressionMammalianMutationdeletion of MIU1 and MIU2PromoterCMVAvailable sinceNov. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHAGE GFP-Ago2 S387E
Plasmid#60655PurposeGFP-Ago2 S387E mutant lentiviral vectorDepositorInsertArgonaute 2 (AGO2 Human)
UseLentiviralTagsGFPExpressionMutationS387EPromoterCMVAvailable sinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-RNF168 delta MIU2
Plasmid#133979Purposemammalian expression vector of Flag tagged RNF168 where the second motif interacting with ubiquitin (MIU) has been deletedDepositorInsertRNF168 (RNF168 Human)
UseTagsFlagExpressionMammalianMutationdeletion of MIU2PromoterCMVAvailable sinceNov. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
mVenus-proα2(I)
Plasmid#119838PurposeExpresses mouse Type I procollagen α2 chain (Col1a2) with mVenus between signal sequence and exon 6DepositorInsertType I procollagen α2 chain (Col1a2 Mouse)
UseTagsmVenusExpressionMammalianMutationCol1a2 exons 2-5 replaced by fluorescent tagPromoterCMVAvailable sinceJan. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCerulean-proα2(I)
Plasmid#119836PurposeExpresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and mCerulean between signal sequence and exon 6DepositorInsertType I procollagen α2 chain (Col1a2 Mouse)
UseTagsmCeruleanExpressionMammalianMutationCol1a2 exons 2-5 replaced by fluorescent tagPromoterCMVAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only